LOCUS V039206 9666 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039206 VERSION V039206 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2058..2175 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" enhancer 2227..2530 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2531..2734 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2854..4815 /label="HSPA5(NM_005347)" /note="HSPA5(NM_005347)" /gene="HSPA5" CDS 4834..4905 /codon_start=1 /product="three tandem FLAG® epitope tags" /transl_table=1 /translation="DYKDDDDKDYKDDDDKDYKDDDDK" /label="3xFLAG" promoter 4923..5422 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5443..6042 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6056..6644 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6719..6952 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" promoter 7157..7261 /gene="bla" /label="AmpR promoter" CDS 7262..8122 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 8293..8881 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 9292..9313 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 9328..9358 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9366..9382 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 9390..9406 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg 2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccac gcgtcgaagc 2821 ccgggcggat cgggtttaaa cggatccgcc accatgaagc tctccctggt ggccgcgatg 2881 ctgctgctgc tcagcgcggc gcgggccgag gaggaggaca agaaggagga cgtgggcacg 2941 gtggtcggca tcgacctggg gaccacctac tcctgcgtcg gcgtgttcaa gaacggccgc 3001 gtggagatca tcgccaacga tcagggcaac cgcatcacgc cgtcctatgt cgccttcact 3061 cctgaagggg aacgtctgat tggcgatgcc gccaagaacc agctcacctc caaccccgag 3121 aacacggtct ttgacgccaa gcggctcatc ggccgcacgt ggaatgaccc gtctgtgcag 3181 caggacatca agttcttgcc gttcaaggtg gttgaaaaga aaactaaacc atacattcaa 3241 gttgatattg gaggtgggca aacaaagaca tttgctcctg aagaaatttc tgccatggtt 3301 ctcactaaaa tgaaagaaac cgctgaggct tatttgggaa agaaggttac ccatgcagtt 3361 gttactgtac cagcctattt taatgatgcc caacgccaag caaccaaaga cgctggaact 3421 attgctggcc taaatgttat gaggatcatc aacgagccta cggcagctgc tattgcttat 3481 ggcctggata agagggaggg ggagaagaac atcctggtgt ttgacctggg tggcggaacc 3541 ttcgatgtgt ctcttctcac cattgacaat ggtgtcttcg aagttgtggc cactaatgga 3601 gatactcatc tgggtggaga agactttgac cagcgtgtca tggaacactt catcaaactg 3661 tacaaaaaga agacgggcaa agatgtcagg aaagacaata gagctgtgca gaaactccgg 3721 cgcgaggtag aaaaggccaa acgggccctg tcttctcagc atcaagcaag aattgaaatt 3781 gagtccttct atgaaggaga agacttttct gagaccctga ctcgggccaa atttgaagag 3841 ctcaacatgg atctgttccg gtctactatg aagcccgtcc agaaagtgtt ggaagattct 3901 gatttgaaga agtctgatat tgatgaaatt gttcttgttg gtggctcgac tcgaattcca 3961 aagattcagc aactggttaa agagttcttc aatggcaagg aaccatcccg tggcataaac 4021 ccagatgaag ctgtagcgta tggtgctgct gtccaggctg gtgtgctctc tggtgatcaa 4081 gatacaggtg acctggtact gcttgatgta tgtcccctta cacttggtat tgaaactgtg 4141 ggaggtgtca tgaccaaact gattccaagg aacacagtgg tgcctaccaa gaagtctcag 4201 atcttttcta cagcttctga taatcaacca actgttacaa tcaaggtcta tgaaggtgaa 4261 agacccctga caaaagacaa tcatcttctg ggtacatttg atctgactgg aattcctcct 4321 gctcctcgtg gggtcccaca gattgaagtc acctttgaga tagatgtgaa tggtattctt 4381 cgagtgacag ctgaagacaa gggtacaggg aacaaaaata agatcacaat caccaatgac 4441 cagaatcgcc tgacacctga agaaatcgaa aggatggtta atgatgctga gaagtttgct 4501 gaggaagaca aaaagctcaa ggagcgcatt gatactagaa atgagttgga aagctatgcc 4561 tattctctaa agaatcagat tggagataaa gaaaagctgg gaggtaaact ttcctctgaa 4621 gataaggaga ccatggaaaa agctgtagaa gaaaagattg aatggctgga aagccaccaa 4681 gatgctgaca ttgaagactt caaagctaag aagaaggaac tggaagaaat tgttcaacca 4741 attatcagca aactctatgg aagtgcaggc cctcccccaa ctggtgaaga ggatacagca 4801 gaaaaagatg agttgctcga gtctagaggg cccgattaca aggatgacga cgataaggat 4861 tacaaggatg acgacgataa ggattacaag gatgacgacg ataagtagtc gagaattcta 4921 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct 4981 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag 5041 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt 5101 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc 5161 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct 5221 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag 5281 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc 5341 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 5401 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc 5461 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 5521 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 5581 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5641 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 5701 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 5761 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5821 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5881 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5941 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6001 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaatcgatag atcctaatca 6061 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 6121 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 6181 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 6241 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 6301 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 6361 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 6421 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 6481 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 6541 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 6601 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga 6661 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 6721 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc 6781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 6841 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 6901 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 6961 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 7021 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat 7081 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 7141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 7201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 7261 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 7321 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 7381 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 7441 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 7501 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 7561 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 7621 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 7681 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 7741 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 7801 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 7861 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 7921 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 7981 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 8041 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 8101 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 8161 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 8221 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 8281 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 8341 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 8401 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 8461 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 8521 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 8581 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 8641 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 8701 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 8761 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 8821 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 8881 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 8941 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 9001 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 9061 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa 9121 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 9181 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc 9241 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 9301 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 9361 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt 9421 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 9481 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc 9541 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga 9601 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt 9661 agtagt //