LOCUS       Exported                2804 bp DNA     circular SYN 06-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2804)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2804)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(51..98)
                     /label=FLP recombinase from the Saccharomyces
                     cerevisi
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(134..772)
                     /codon_start=1
                     /product="Chloramphenicol acetyltransferase"
                     /label=CmR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR"
     promoter        complement(773..875)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(981..1014)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(1063..1446)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1519..2376)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2377..2481)
                     /label=AmpR promoter
     terminator      2572..2658
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2750..2777
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
       61 tactttctag agaataggaa cttcggaata ggaacttcat ttaaatggcg cgccttacgc
      121 cccgccctgc cactcatcgc agtactgttg tattcattaa gcatctgccg acatggaagc
      181 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      241 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      301 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
      361 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      421 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
      481 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
      541 tcattgccat acgtaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
      601 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
      661 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
      721 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
      781 ccttagctcc tgaaaatctc gacaactcaa aaaatacgcc cggtagtgat cttatttcat
      841 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
      901 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
      961 ccgtcacagg taggcgcgcc gaagttccta tactttctag agaataggaa cttcggaata
     1021 ggaactaagg aggatattca tatggaccat ggctaattcc catgtcagcc gttaagtgtt
     1081 cctgtgtcac tgaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct
     1141 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt
     1201 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt
     1261 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc
     1321 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat
     1381 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgcgg
     1441 atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat cgatggcccc
     2521 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa
     2581 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2641 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     2701 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc
     2761 ctgacggatg gcctttttgc gtggccagtg ccaagcttgc atgc
//
