LOCUS       Exported               10475 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  MoClo level 2 destination vector pL2-1, complete sequence.
ACCESSION   HQ728317
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs
  JOURNAL   PLoS ONE 6 (2), E16765 (2011)
  PUBMED    21364738
REFERENCE   2  (bases 1 to 10475)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22, 
            Halle/Saale 06120, Germany
REFERENCE   3  (bases 1 to 10475)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="MoClo level 2 destination vector pL2-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:993666"
     misc_feature    1..4
                     /note="fusion site; other site"
     misc_feature    23..47
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(170..758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(929..1723)
                     /codon_start=1
                     /gene="aphA"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aphA
                     /protein_id="ADZ30886.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(929..1723)
                     /gene="aphA"
                     /label=aphA
     promoter        complement(1724..1828)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1909..4693)
                     /direction=LEFT
                     /label=pVS1 ori
                     /note="pVS1 ori"
     rep_origin      2111..2305
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2371..3438)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(3872..4501)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4765..4789
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    4862..4865
                     /note="fusion site; other site"
     CDS             5115..6023
                     /codon_start=1
                     /gene="crtE"
                     /product="geranylgeranyl pyrophosphate synthase"
                     /label=crtE
                     /note="GGPP"
                     /protein_id="ADZ30887.1"
                     /translation="MTVCAKKHVHLTRDAAEQLLADIDRRLDQLLPVEGERDVVGAAMR
                     EGALAPGKRIRPMLLLLTARDLGCAVSHDGLLDLACAVEMVHAASLILDDMPCMDDAKL
                     RRGRPTIHSHYGEHVAILAAVALLSKAFGVIADADGLTPLAKNRAVSELSNAIGMQGLV
                     QGQFKDLSEGDKPRSAEAILMTNHFKTSTLFCASMQMASIVANASSEARDCLHRFSLDL
                     GQAFQLLDDLTDGMTDTGKDSNQDAGKSTLVNLLGPRAVEERLRQHLQLASEHLSAACQ
                     HGHATQHFIQAWFDKKLAAVS"
     gene            5115..6023
                     /gene="crtE"
                     /label=crtE
     CDS             6033..6761
                     /codon_start=1
                     /gene="crtW"
                     /product="beta-carotene ketolase"
                     /label=crtW
                     /protein_id="ADZ30888.1"
                     /translation="MSAHALPKADLTATSLIVSGGIIAAWLALHVHALWFLDAAAHPIL
                     AIANFLGLTWLSVGLFIIAHDAMHGSVVPGRPRANAAMGQLVLWLYAGFSWRKMIVKHM
                     AHHRHAGTDDDPDFDHGGPVRWYARFIGTYFGWREGLLLPVIVTVYALILGDRWMYVVF
                     WPLPSILASIQLFVFGTWLPHRPGHDAFPDRHNARSSRISDPVSLLTCFHFGGYHHEHH
                     LHPTVPWWRLPSTRTKGDTA"
     gene            6033..6761
                     /gene="crtW"
                     /label=crtW
     regulatory      6389..6398
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6783..7931
                     /codon_start=1
                     /gene="crtY"
                     /product="lycopene cyclase"
                     /label=crtY
                     /protein_id="ADZ30889.1"
                     /translation="MQPHYDLILVGAGLANGLIALRLQQQQPDMRILLIDAAPQAGGNH
                     TWSFHHDDLTESQHRWIAPLVVHHWPDYQVRFPTRRRKLNSGYFCITSQRFAEVLQRQF
                     GPHLWMDTAVAEVNAESVRLKKGQVIGARAVIDGRGYAANSALSVGFQAFIGQEWRLSH
                     PHGLSSPIIMDATVDQQNGYRFVYSLPLSPTRLLIEDTHYIDNATLDPECARQNICDYA
                     AQQGWQLQTLLREEQGALPITLSGNADAFWQQRPLACSGLRAGLFHPTTGYSLPLAVAV
                     ADRLSALDVFTSASIHHAITHFARERWQQQGFFRMLNRMLFLAGPADSRWRVMQRFYGL
                     PEDLIARFYAGKLTLTDRLRILSGKPPVPVLAALQAIMTTHR"
     gene            6783..7931
                     /gene="crtY"
                     /label=crtY
     CDS             7943..9421
                     /codon_start=1
                     /gene="crtI"
                     /product="phytoene dehydrogenase"
                     /label=crtI
                     /protein_id="ADZ30890.1"
                     /translation="MKPTTVIGAGFGGLALAIRLQAAGIPVLLLEQRDKPGGRAYVYED
                     QGFTFDAGPTVITDPSAIEELFALAGKQLKEYVELLPVTPFYRLCWESGKVFNYDNDQT
                     RLEAQIQQFNPRDVEGYRQFLDYSRAVFKEGYLKLGTVPFLSFRDMLRAAPQLAKLQAW
                     RSVYSKVASYIEDEHLRQAFSFHSLLVGGNPFATSSIYTLIHALEREWGVWFPRGGTGA
                     LVQGMIKLFQDLGGEVVLNARVSHMETTGNKIEAVHLEDGRRFLTQAVASNADVVHTYR
                     DLLSQHPAAVKQSNKLQTKRMSNSLFVLYFGLNHHHDQLAHHTVCFGPRYRELIDEIFN
                     HDGLAEDFSLYLHAPCVTDSSLAPEGCGSYYVLAPVPHLGTANLDWTVEGPKLRDRIFA
                     YLEQHYMPGLRSQLVTHRMFTPFDFRDQLNAYHGSAFSVEPVLTQSAWFRPHNRDKTIT
                     NLYLVGAGTHPGAGIPGVIGSAKATAGLMLEDLI"
     gene            7943..9421
                     /gene="crtI"
                     /label=crtI
     CDS             9457..10347
                     /codon_start=1
                     /gene="crtB"
                     /product="phytoene synthase"
                     /label=crtB
                     /protein_id="ADZ30891.1"
                     /translation="MAVGSKSFATASKLFDAKTRRSVLMLYAWCRHCDDVIDDQTLGFQ
                     ARQPALQTPEQRLMQLEMKTRQAYAGSQMHEPAFAAFQEVAMAHDIAPAYAFDHLEGFA
                     MDVREAQYSQLDDTLRYCYHVAGVVGLMMAQIMGVRDNATLDRACDLGLAFQLTNIARD
                     IVDDAHAGRCYLPASWLEHEGLNKENYAAPENRQALSRIARRLVQEAEPYYLSATAGLA
                     GLPLRSAWAIATAKQVYRKIGVKVEQAGQQAWDQRQSTTTPEKLTLLLAASGQALTSRM
                     RAHPPRPAHLWQRPL"
     gene            9457..10347
                     /gene="crtB"
                     /label=crtB
ORIGIN
        1 gggagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
       61 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
      121 ttgtatgtgc caaccacagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      601 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      901 gtatatatga gtaaacttgg tctgacagct aaaacaattc atccagtaaa atataatatt
      961 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     1021 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     1081 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     1141 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     1201 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ctcttcccag ttttcgcaat
     1261 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     1321 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     1381 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     1441 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     1501 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     1561 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     1621 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     1681 cgatcagttt tttcaattcc ggtgatattc tcattttagc catactcttc ctttttcaat
     1741 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     1801 agaaaaataa acaaataggg gttccgcgca cgaattggcc agcgctgcca tttttggggt
     1861 gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggagctcgg tgccttgatg
     1921 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     1981 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     2041 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     2101 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     2161 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     2221 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     2281 gttcccaatg tacgtgctat ccacaggaaa gaagcttttt cgaccttttt cccctgctag
     2341 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     2401 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     2461 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     2521 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     2581 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     2641 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     2701 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
     2761 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
     2821 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
     2881 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
     2941 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
     3001 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
     3061 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
     3121 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
     3181 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
     3241 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
     3301 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
     3361 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
     3421 gccggatggt ttgcgaccgt cacgccgatt cctcgggctt gggggttcca gtgccattgc
     3481 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
     3541 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
     3601 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
     3661 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
     3721 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
     3781 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
     3841 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
     3901 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
     3961 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
     4021 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
     4081 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
     4141 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
     4201 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
     4261 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
     4321 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
     4381 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
     4441 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
     4501 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
     4561 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
     4621 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
     4681 atgcagccgc acggctgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     4741 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     4801 tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat gcagtgggcc
     4861 ctgccttgtc ttctggtcga ctcgacggag ggtaccgcac ggtctgccaa tccgacggag
     4921 gtttatgaat tttccacctt ttccacaagc tcaactagta ttaacgatgt ggatttagca
     4981 aaaaaaacct gtaaccctaa atgtaaaata acgggtaagc ctgccaacca tgttatggca
     5041 gattaagcgt ctttttgaag ggcaccgcat ctttcgcgtt gccgtaaatg tatccgttta
     5101 taaggacagc ccgaatgacg gtctgcgcaa aaaaacacgt tcatctcact cgcgatgctg
     5161 cggagcagtt actggctgat attgatcgac gccttgatca gttattgccc gtggagggag
     5221 aacgggatgt tgtgggtgcc gcgatgcgtg aaggtgcgct ggcaccggga aaacgtattc
     5281 gccccatgtt gctgttgctg accgcccgcg atctgggttg cgctgtcagc catgacggat
     5341 tactggattt ggcctgtgcg gtggaaatgg tccacgcggc ttcgctgatc cttgacgata
     5401 tgccctgcat ggacgatgcg aagctgcggc gcggacgccc taccattcat tctcattacg
     5461 gagagcatgt ggcaatactg gcggcggttg ccttgctgag taaagccttt ggcgtaattg
     5521 ccgatgcaga tggcctcacg ccgctggcaa aaaatcgggc ggtttctgaa ctgtcaaacg
     5581 ccatcggcat gcaaggattg gttcagggtc agttcaagga tctgtctgaa ggggataagc
     5641 cgcgcagcgc tgaagctatt ttgatgacga atcactttaa aaccagcacg ctgttttgtg
     5701 cctccatgca gatggcctcg attgttgcga atgcctccag cgaagcgcgt gattgcctgc
     5761 atcgtttttc acttgatctt ggtcaggcat ttcaactgct ggacgatttg accgatggca
     5821 tgaccgacac cggtaaggat agcaatcagg acgccggtaa atcgacgctg gtcaatctgt
     5881 taggcccgag ggcggttgaa gaacgtctga gacaacatct tcagcttgcc agtgagcatc
     5941 tctctgcggc ctgccaacac gggcacgcca ctcaacattt tattcaggcc tggtttgaca
     6001 aaaaactcgc tgccgtcagt taaggatgct gcatgagcgc acatgccctg cccaaggcag
     6061 atctgaccgc caccagcctg atcgtatcgg gcggcatcat cgccgcttgg ctggccctgc
     6121 atgtgcatgc gctgtggttt ctggacgcag cggcgcatcc catcctggcg atcgcaaatt
     6181 tcctggggct gacctggctg tcggtcggat tgttcatcat cgcgcatgac gcgatgcacg
     6241 ggtcggtggt gccggggcgt ccgcgcgcca atgcggcgat gggccagctt gtcctgtggc
     6301 tgtatgccgg attttcgtgg cgcaagatga tcgtcaagca catggcccat caccgccatg
     6361 ccggaaccga cgacgacccc gatttcgacc atggcggccc ggtccgctgg tacgcccgct
     6421 tcatcggcac ctatttcggc tggcgcgagg ggctgcttct gcccgtcatc gtgacggtct
     6481 atgcgctgat ccttggggat cgctggatgt acgtggtatt ctggccgctg ccgtcgatcc
     6541 tggcgtcgat ccagctgttc gtgttcggca cctggctgcc gcaccgcccc ggccacgacg
     6601 cgttcccgga ccgccacaat gcgcggtcgt cgcggatcag cgaccccgtg tcgctgctga
     6661 cctgttttca ctttggcggt tatcatcacg aacaccacct gcacccgacg gtgccgtggt
     6721 ggcgcctgcc cagcacccgc accaaggggg acaccgcatg agtgacttaa gtgggagcgg
     6781 ctatgcaacc gcattatgat ctgattctcg tgggggctgg actcgcgaat ggccttatcg
     6841 ccctgcgact tcagcagcag caacctgata tgcgtatttt gcttatcgac gccgcacccc
     6901 aggcgggcgg gaatcatacg tggtcatttc accacgatga tttgactgag agccaacatc
     6961 gttggatagc tccgctggtg gttcatcact ggcccgacta tcaggtacgc tttcccacac
     7021 gccgtcgtaa gctgaacagc ggctactttt gtattacttc tcagcgtttc gctgaggttt
     7081 tacagcgaca gtttggcccg cacttgtgga tggataccgc ggtcgcagag gttaatgcgg
     7141 aatctgttcg gttgaaaaag ggtcaggtta tcggtgcccg cgcggtgatt gacgggcggg
     7201 gttatgcggc aaattcagca ctgagcgtgg gcttccaggc gtttattggc caggaatggc
     7261 gattgagcca cccgcatggt ttatcatctc ccattatcat ggatgccacg gtcgatcagc
     7321 aaaatggtta tcgcttcgtg tacagcctgc cgctctcgcc gaccagattg ttaattgaag
     7381 atacgcacta tattgataat gcgacattag atcctgaatg cgcgcggcaa aatatttgcg
     7441 actatgccgc gcaacagggt tggcagcttc agacactgct gcgagaagaa cagggcgcct
     7501 tacccattac tctgtcgggc aatgccgacg cattctggca gcagcgcccc ctggcctgta
     7561 gtggattacg tgccggtctg ttccatccta ccaccggcta ttcactgccg ctggcggttg
     7621 ccgtggccga ccgcctgagt gcacttgatg tctttacgtc ggcctcaatt caccatgcca
     7681 ttacgcattt tgcccgcgag cgctggcagc agcagggctt tttccgcatg ctgaatcgca
     7741 tgctgttttt agccggaccc gccgattcac gctggcgggt tatgcagcgt ttttatggtt
     7801 tacctgaaga tttaattgcc cgtttttatg cgggaaaact cacgctgacc gatcggctac
     7861 gtattctgag cggcaagccg cctgttccgg tattagcagc attgcaagcc attatgacga
     7921 ctcatcgtta aagagcgact acatgaaacc aactacggta attggtgcag gcttcggtgg
     7981 cctggcactg gcaattcgtc tacaagctgc ggggatcccc gtcttactgc ttgaacaacg
     8041 tgataaaccc ggcggtcggg cttatgtcta cgaggatcag gggtttacct ttgatgcagg
     8101 cccgacggtt atcaccgatc ccagtgccat tgaagaactg tttgcactgg caggaaaaca
     8161 gttaaaagag tatgtcgaac tgctgccggt tacgccgttt taccgcctgt gttgggagtc
     8221 agggaaggtc tttaattacg ataacgatca aacccggctc gaagcgcaga ttcagcagtt
     8281 taatccccgc gatgtcgaag gttatcgtca gtttctggac tattcacgcg cggtgtttaa
     8341 agaaggctat ctaaagctcg gtactgtccc ttttttatcg ttcagagaca tgcttcgcgc
     8401 cgcacctcaa ctggcgaaac tgcaggcatg gagaagcgtt tacagtaagg ttgccagtta
     8461 catcgaagat gaacatctgc gccaggcgtt ttctttccac tcgctgttgg tgggcggcaa
     8521 tcccttcgcc acctcatcca tttatacgtt gatacacgcg ctggagcgtg agtggggcgt
     8581 ctggtttccg cgtggcggca ccggcgcatt agttcagggg atgataaagc tgtttcagga
     8641 tctgggtggc gaagtcgtgt taaacgccag agtcagccat atggaaacga caggaaacaa
     8701 gattgaagcc gtgcatttag aggacggtcg caggttcctg acgcaagccg tcgcgtcaaa
     8761 tgcagatgtg gttcatacct atcgcgacct gttaagccag caccctgccg cggttaagca
     8821 gtccaacaaa ctgcagacta agcgcatgag taactctctg tttgtgctct attttggttt
     8881 gaatcaccat catgatcagc tcgcgcatca cacggtttgt ttcggcccgc gttaccgcga
     8941 gctgattgac gaaattttta atcatgatgg cctcgcagag gacttctcac tttatctgca
     9001 cgcgccctgt gtcacggatt cgtcactggc gcctgaaggt tgcggcagtt actatgtgtt
     9061 ggcgccggtg ccgcatttag gcaccgcgaa cctcgactgg acggttgagg ggccaaaact
     9121 acgcgaccgt atttttgcgt accttgagca gcattacatg cctggcttac ggagtcagct
     9181 ggtcacgcac cggatgttta cgccgtttga ttttcgcgac cagcttaatg cctatcatgg
     9241 ctcagccttt tctgtggagc ccgttcttac ccagagcgcc tggtttcggc cgcataaccg
     9301 cgataaaacc attactaatc tctacctggt cggcgcaggc acgcatcccg gcgcaggcat
     9361 tcctggcgtc atcggctcgg caaaagcgac agcaggtttg atgctggagg atctgatttg
     9421 aataatccgt cgttactcaa tcatgcggtc gaaacgatgg cagttggctc gaaaagtttt
     9481 gcgacagcct caaagttatt tgatgcaaaa acccggcgca gcgtactgat gctctacgcc
     9541 tggtgccgcc attgtgacga tgttattgac gatcagacgc tgggctttca ggcccggcag
     9601 cctgccttac aaacgcccga acaacgtctg atgcaacttg agatgaaaac gcgccaggcc
     9661 tatgcaggat cgcagatgca cgaaccggcg tttgcggctt ttcaggaagt ggctatggct
     9721 catgatatcg ccccggctta cgcgtttgat catctggaag gcttcgccat ggatgtacgc
     9781 gaagcgcaat acagccaact ggatgatacg ctgcgctatt gctatcacgt tgcaggcgtt
     9841 gtcggcttga tgatggcgca aatcatgggc gtgcgggata acgccacgct ggaccgcgcc
     9901 tgtgaccttg ggctggcatt tcagttgacc aatattgctc gcgatattgt ggacgatgcg
     9961 catgcgggcc gctgttatct gccggcaagc tggctggagc atgaaggtct gaacaaagag
    10021 aattatgcgg cacctgaaaa ccgtcaggcg ctgagccgta tcgcccgtcg tttggtgcag
    10081 gaagcagaac cttactattt gtctgccaca gccggcctgg cagggttgcc cctgcgttcc
    10141 gcctgggcaa tcgctacggc gaagcaggtt taccggaaaa taggtgtcaa agttgaacag
    10201 gccggtcagc aagcctggga tcagcggcag tcaacgacca cgcccgaaaa attaacgctg
    10261 ctgctggccg cctctggtca ggcccttact tcccggatgc gggctcatcc tccccgccct
    10321 gcgcatctct ggcagcgccc gctctagcgc catgtctttc ccggagcgtc gcctgaagtt
    10381 ttgacagggg cggcgcatag aggaagccaa aagaaacaca accttctttg cccctgacgg
    10441 cgtgatgcat acgctggcga ttagcacgaa gacaa
//
