LOCUS       V046410                10672 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046410
VERSION     V046410
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        9..235
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             236..416
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    463..588
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1081..1314
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1499..1543
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1810..1926
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2130..3312
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     promoter        3329..3347
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             3369..5429
                     /label="Pip5k1c(NM_001293646)"
                     /note="Pip5k1c(NM_001293646)"
                     /gene="Pip5k1c"
     CDS             5430..5495
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5504..6077
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             6111..6509
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6524..7112
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7199..7432
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7504..7625
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7665..7800
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(7821..7839)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(7849..7865)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      8007..8462
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8488..8592
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8593..9453
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9624..10212
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    10500..10521
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        10536..10566
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10574..10590
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     10598..10614
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        10635..10653
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat
     3361 ccgccaccat ggagctagag gtgccggacg aggcggagag cgccgaagcg ggggccgtga
     3421 cggcggaagc ggcctggtct gcggagagtg gggcggcggc aggtatgacc cagaagaagg
     3481 ctggccttgc agaggccccc ttggtgacag ggcagccagg ccctggccat gggaagaagc
     3541 tgggccaccg aggcgtggat gcgtcgggag agactacgta taagaagacc acctcgtcca
     3601 ccctgaaggg cgccatccag ctggggatcg ggtacacggt gggcaacctg agctctaagc
     3661 cggagcggga cgtgctcatg caggacttct acgtggtgga gagcatcttc ttccccagtg
     3721 aagggagcaa cctcacccct gcccaccact tccaggattt ccgcttcaag acctatgcac
     3781 ctgttgcctt ccgctacttc cgggagctct ttggcatccg tccagatgac tatttgtact
     3841 ccctgtgcaa tgagccactc atcgagctct ccaaccccgg ggccagcggc tctgtcttct
     3901 acgtcaccag cgacgacgag ttcatcatca agactgtcat gcacaaggag gcagagttcc
     3961 tgcagaagct gctgcctggc tactacatga atctcaacca gaacccacgg acgctgctgc
     4021 ccaagttcta tgggctgtac tgcgtgcagt ctggtggcaa gaacatccgc gtggtggtca
     4081 tgaacaatgt gctgccccgt gtcgtcaaaa tgcaccttaa gttcgacctc aagggctcca
     4141 cgtacaagcg cagggccagc aagaaggaga aggagaagag cctgcccacc tacaaggacc
     4201 tggacttcat gcaggacatg cccgaggggc tgctgctgga ctccgacacc tttggcgccc
     4261 tggtcaagac gctgcagcga gactgcctgg tgctggagag cttcaagata atggactaca
     4321 gcctgctgct gggcgtgcac aacatcgatc agcaggagcg agagcgccag gccgagggcg
     4381 cccagagcaa ggcggatgag aagcggcccg tggcccagaa ggctctgtat tccacggcca
     4441 tggagtctat ccagggcgga gctgcccgtg gggaggccat tgagacagat gacacgatgg
     4501 gtgggattcc agcagtgaat gggcgcgggg agcgactgct cctccacatc gggatcattg
     4561 atattctgca gtcctacagg ttcatcaaga agttagaaca cacctggaag gccctcgtcc
     4621 atgatgggga cactgtctca gtccaccggc ccagcttcta tgcagagcgc ttcttcaagt
     4681 tcatgagcag cacggtgttc cggaagagtt cctccctgaa gtcctctcca tccaagaaag
     4741 gacgtggtgc cctgctggcg gtcaaacccc tggggcccac tgctgccttc tcagccagcc
     4801 agatccccag cgagagagag gatgtgcagt atgacctgcg gggggcccgc agctacccca
     4861 cgcttgagga tgaaggccgg cctgacctcc tgccctgcac cccaccgtcc tttgaggaag
     4921 ccaccacagc ctccatcgcc accaccctgt cgtccacctc cctctccatc ccagagcggt
     4981 ccccttcaga tacatcggag cagccccggt acaggcggcg cacgcagtct tcaggccagg
     5041 atggccggcc ccaggaggag ccccatgcgg aagacctgca gaagataact gtgcaggtgg
     5101 agccagtgtg cggtgtgggg gttgtcccca aggaggaggg tgcaggagtg gaggtccccc
     5161 catgtggggc atcggctgca gcctctgtgg aaatagacgc tgccagccag gcctcagagc
     5221 ctgccagcca ggcctcagat gaggaggatg caccctctac agacatctat tttttcgccc
     5281 atgggagata ctggcttttc tctccccgtc gtcgccaact gcgggccgtg acaccaaacc
     5341 acacaggcac tcccaccgac gagaggagct gggtgtactc cccgcttcac tatagcgcgc
     5401 ggcccgcctc cgacggcgag agcgacacag actacaaaga ccatgacggt gattataaag
     5461 atcatgacat cgactacaag gatgacgatg acaagtaggc tagcccctct ccctcccccc
     5521 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5581 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5641 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5701 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5761 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5821 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5881 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5941 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
     6001 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6061 gaaaaacacg atgataatat ggccacacta gagatccacc ggtcgccacc atggccaagc
     6121 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     6181 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     6241 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     6301 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     6361 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     6421 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     6481 tgccctctgg ttatgtgtgg gagggctaag cggccgcgtc gacaatcaac ctctggatta
     6541 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6601 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6661 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6721 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6781 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6841 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6901 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6961 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7021 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7081 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga
     7141 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7201 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7261 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7321 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7381 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7441 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7501 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7621 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     7681 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7741 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7801 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     7861 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7921 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7981 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     8041 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8101 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8161 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8221 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8281 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8341 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8401 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8461 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8521 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8581 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8641 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8701 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8761 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8821 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8881 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8941 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9001 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9061 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9121 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9181 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9241 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9301 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9361 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9421 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9481 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9541 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9601 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9661 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9721 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9781 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9841 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9901 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9961 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10021 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10081 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10141 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10201 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10261 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10321 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10381 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10441 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10501 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    10561 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    10621 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gc
//
