LOCUS       V033606                12516 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033606
VERSION     V033606
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1972
                     /label="ASB16(NM_080863)"
                     /note="ASB16(NM_080863)"
                     /gene="ASB16"
     CDS             1973..1999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2045..2386
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2512..3011
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3032..3739
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3740..3796
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3797..4396
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4407..4995
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5058..5291
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5316..5334)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6123..6227
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6228..7088
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7259..7847
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8093..8422
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9556..9621
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9751..9771
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10196..10330
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10499..11132
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11179..11304
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11796..12029
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12214..12258
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttccgag tgggcagtga
      601 actccaagcc accatggcaa gagagacctt ccccttcacc tcctccatgc tgcgctctct
      661 ccgcctgcag caggagtggc tggaatggga ggaccggcgg cgggcggctg cccagcagtg
      721 ccggagccgc aggtgcccgt caagtccccg ggcccgactc actaggcctc accgttcctg
      781 ccgagaccca gctgtccacc aagccctctt ctccggcaac ctgcagcagg tccaagccct
      841 gttccaagat gaagaggccg ccaacatgat tgtggagact gtgagcaacc agctggcctg
      901 gtcggctgaa caggggttct gggtgctgac ccccaagacc aagcagacgg cacccctcgc
      961 catcgctaca gcccgaggct acacagactg tgctcgacac ctgatccggc agggagctga
     1021 gctggatgcc cgtgtcgggg gtcgcgctgc cttgcatgag gcctgtgccc gagcccagtt
     1081 tgactgtgtg cggctgctgc tgaccttcgg agccaaggct aatgtgctga ctgaggaggg
     1141 cacgactcct ttgcacctct gcacgatccc cgagtccttg cagtgcgcca agttgctgct
     1201 ggaagcagga gcgacggtga acctggcagc aggcgagagc caggagacgc ccctgcacgt
     1261 ggcggcggcg cgcggcctgg agcaacatgt ggctctgtac ctggagcatg gcgccgacgt
     1321 gggcctgcgc accagccagg gcgagactgc gctgaacacg gcgtgcgctg gggccgaggg
     1381 cccaggtagc tgcaggcgac accaggctgc ggcgcgccgg ctcctggagg ctggagctga
     1441 tgcccgggcg gccgggcgca agcgccacac gccgctgcac aacgcttgtg ccaacggctg
     1501 cgggggcctg gccgagctgc tgctgcgtta cggggcccgc gctgaggtcc ccaatggggc
     1561 gggccacacg cccatggact gtgcgctgca ggccgtccag gactccccca actgggagcc
     1621 tgaagtcctt ttcgccgcac tgctggacta cggggcgcag ccagtgcgcc ctgagatgct
     1681 gaaacactgc gccaacttcc ctcgggccct ggaagtcctg cttaatgcct atccttgtgt
     1741 cccatcctgt gagacctggg tggaggcggt gctcccagag ctgtggaagg agcacgaagc
     1801 cttctacagc tcggccctgt gcatggtgaa ccagccaagg cagctgcagc acctggcccg
     1861 actagctgtg cgcgctcggt tgggaagccg ctgccggcag ggtgccaccc ggctgccact
     1921 gcccccgctc ctcagggact acctgctgct gcgtgtggag gggtgcatcc agtaccccta
     1981 cgacgtgcct gactacgccg gcggcggtgg ttcaggcggt ggcggttccg gtggtggggg
     2041 atcagtccaa ctggtggagt ctggtggcgc tttggtgcag ccaggtggct ctctgcgttt
     2101 gtcctgtgcc gcttctggct tcccagtgaa ccgctattcc atgcgctggt atcgccaggc
     2161 tccaggcaaa gagcgtgagt gggtagccgg tatgtccagc gcgggtgatc gtagctccta
     2221 tgaagactcc gtgaagggcc gtttcaccat cagccgtgac gatgcccgta acacggtgta
     2281 tctgcaaatg aacagcttga aacctgaaga tacggccgtg tattactgta atgtgaacgt
     2341 gggcttcgag tattggggcc aaggcaccca ggtcaccgtc tccagctaag atccttgact
     2401 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
     2461 acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac cgggtagggg
     2521 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     2581 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     2641 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     2701 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     2761 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     2821 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     2881 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     2941 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     3001 cgggcctttc gacctgcagc ccaagcttac catggtgagc aagggcgagg aggataacat
     3061 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3121 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3181 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3241 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3301 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     3361 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     3421 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3481 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     3541 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3601 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3661 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3721 catggacgag ctgtacaagg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     3781 agagaatcct ggaccgatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     3841 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     3901 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     3961 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4021 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     4081 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4141 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     4201 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     4261 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     4321 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     4381 caagcccggt gcctgatctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     4441 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4501 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4561 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4621 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4681 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4741 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4801 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4861 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4921 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4981 gggccgcctc cccgcatcga taccgtcgac caaggcagct gtagatctta gccacttttt
     5041 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5101 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5161 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5221 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5281 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     5341 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     5401 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     5461 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     5521 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     5581 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     5641 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     5701 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     5761 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     5821 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctagctct gatgccgcat
     5881 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5941 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6001 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     6061 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6181 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6241 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6301 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6361 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6421 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6481 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6541 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6601 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6661 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6721 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6781 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6841 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6901 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6961 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7021 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7081 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7141 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7201 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7261 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7321 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7381 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7441 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7501 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7561 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7621 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7681 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7741 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7801 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7861 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7921 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7981 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8041 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     8101 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8161 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8221 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8281 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8341 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8401 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     8461 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     8521 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     8581 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     8641 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     8701 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     8761 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     8821 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     8881 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     8941 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     9001 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     9061 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     9121 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     9181 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     9241 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     9301 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     9361 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
     9421 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
     9481 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     9541 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     9601 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     9661 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     9721 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     9781 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     9841 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     9901 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     9961 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    10021 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    10081 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    10141 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    10201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10321 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    10381 ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg
    10441 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
    10501 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
    10561 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
    10621 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
    10681 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    10741 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
    10801 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
    10861 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
    10921 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
    10981 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    11041 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    11101 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    11161 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    11221 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    11281 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    11341 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    11401 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    11461 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    11521 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    11581 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    11641 gcggccggtg atcttcagac ctggacgata tatatgaggg acaattggag aagtgaatta
    11701 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    11761 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    11821 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    11881 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    11941 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    12001 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    12061 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    12121 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    12181 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    12241 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    12301 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    12361 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    12421 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    12481 agatccattc gattagtgaa cggatctcga cggtcg
//
