LOCUS       V030317                13488 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030317
VERSION     V030317
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2944
                     /label="BARD1(NM_000465)"
                     /note="BARD1(NM_000465)"
                     /gene="BARD1"
     CDS             2945..2971
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3017..3358
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3484..3983
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4004..4711
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4712..4768
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4769..5368
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5379..5967
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6030..6263
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6288..6306)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7095..7199
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7200..8060
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8231..8819
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9065..9394
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10528..10593
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10723..10743
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11168..11302
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11471..12104
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12151..12276
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12768..13001
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13186..13230
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgacact cgggcagtga
      601 actccaagcc accatgccgg ataatcggca gccgaggaac cggcagccga ggatccgctc
      661 cgggaacgag cctcgttccg cgcccgccat ggaaccggat ggtcgcggtg cctgggccca
      721 cagtcgcgcc gcgctcgacc gcctggagaa gctgctgcgc tgctcgcgtt gtactaacat
      781 tctgagagag cctgtgtgtt taggaggatg tgagcacatc ttctgtagta attgtgtaag
      841 tgactgcatt ggaactggat gtccagtgtg ttacaccccg gcctggatac aagacttgaa
      901 gataaataga caactggaca gcatgattca actttgtagt aagcttcgaa atttgctaca
      961 tgacaatgag ctgtcagatt tgaaagaaga taaacctagg aaaagtttgt ttaatgatgc
     1021 aggaaacaag aagaattcaa ttaaaatgtg gtttagccct cgaagtaaga aagtcagata
     1081 tgttgtgagt aaagcttcag tgcaaaccca gcctgcaata aaaaaagatg caagtgctca
     1141 gcaagactca tatgaatttg tttccccaag tcctcctgca gatgtttctg agagggctaa
     1201 aaaggcttct gcaagatctg gaaaaaagca aaaaaagaaa actttagctg aaatcaacca
     1261 aaaatggaat ttagaggcag aaaaagaaga tggtgaattt gactccaaag aggaatctaa
     1321 gcaaaagctg gtatccttct gtagccaacc atctgttatc tccagtcctc agataaatgg
     1381 tgaaatagac ttactagcaa gtggctcctt gacagaatct gaatgttttg gaagtttaac
     1441 tgaagtctct ttaccattgg ctgagcaaat agagtctcca gacactaaga gcaggaatga
     1501 agtagtgact cctgagaagg tctgcaaaaa ttatcttaca tctaagaaat ctttgccatt
     1561 agaaaataat ggaaaacgtg gccatcacaa tagactttcc agtcccattt ctaagagatg
     1621 tagaaccagc attctgagca ccagtggaga ttttgttaag caaacggtgc cctcagaaaa
     1681 tataccattg cctgaatgtt cttcaccacc ttcatgcaaa cgtaaagttg gtggtacatc
     1741 agggaggaaa aacagtaaca tgtccgatga attcattagt ctttcaccag gtacaccacc
     1801 ttctacatta agtagttcaa gttacaggcg agtgatgtct agtccctcag caatgaagct
     1861 gttgcccaat atggctgtga aaagaaatca tagaggagag actttgctcc atattgcttc
     1921 tattaagggc gacatacctt ctgttgaata ccttttacaa aatggaagtg atccaaatgt
     1981 taaagaccat gctggatgga caccattgca tgaagcttgc aatcatgggc acctgaaggt
     2041 agtggaatta ttgctccagc ataaggcatt ggtgaacacc accgggtatc aaaatgactc
     2101 accacttcac gatgcagcca agaatgggca tgtggatata gtcaagctgt tactttccta
     2161 tggagcctcc agaaatgctg ttaatatatt tggtctgcgg cctgtcgatt atacagatga
     2221 tgaaagtatg aaatcgctat tgctgctacc agagaagaat gaatcatcct cagctagcca
     2281 ctgctcagta atgaacactg ggcagcgtag ggatggacct cttgtactta taggcagtgg
     2341 gctgtcttca gaacaacaga aaatgctcag tgagcttgca gtaattctta aggctaaaaa
     2401 atatactgag tttgacagta cagtaactca tgttgttgtt cctggtgatg cagttcaaag
     2461 taccttgaag tgtatgcttg ggattctcaa tggatgctgg attctaaaat ttgaatgggt
     2521 aaaagcatgt ctacgaagaa aagtatgtga acaggaagaa aagtatgaaa ttcctgaagg
     2581 tccacgcaga agcaggctca acagagaaca gctgttgcca aagctgtttg atggatgcta
     2641 cttctatttg tggggaacct tcaaacacca tccaaaggac aaccttatta agctcgtcac
     2701 tgcaggtggg ggccagatcc tcagtagaaa gcccaagcca gacagtgacg tgactcagac
     2761 catcaataca gtcgcatacc atgcgagacc cgattctgat cagcgcttct gcacacagta
     2821 tatcatctat gaagatttgt gtaattatca cccagagagg gttcggcagg gcaaagtctg
     2881 gaaggctcct tcgagctggt ttatagactg tgtgatgtcc tttgagttgc ttcctcttga
     2941 cagctacccc tacgacgtgc ctgactacgc cggcggcggt ggttcaggcg gtggcggttc
     3001 cggtggtggg ggatcagtcc aactggtgga gtctggtggc gctttggtgc agccaggtgg
     3061 ctctctgcgt ttgtcctgtg ccgcttctgg cttcccagtg aaccgctatt ccatgcgctg
     3121 gtatcgccag gctccaggca aagagcgtga gtgggtagcc ggtatgtcca gcgcgggtga
     3181 tcgtagctcc tatgaagact ccgtgaaggg ccgtttcacc atcagccgtg acgatgcccg
     3241 taacacggtg tatctgcaaa tgaacagctt gaaacctgaa gatacggccg tgtattactg
     3301 taatgtgaac gtgggcttcg agtattgggg ccaaggcacc caggtcaccg tctccagcta
     3361 agatccttga cttgcggccg caactcccac ctgcaacatg cgtgactgac tgaggccgcg
     3421 actctagagt cgacctgcat ctaggcgccg gaattagatc tctcgaggtt aacgaattct
     3481 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     3541 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     3601 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     3661 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     3721 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     3781 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     3841 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     3901 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     3961 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatggtga gcaagggcga
     4021 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     4081 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     4141 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     4201 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     4261 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     4321 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     4381 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     4441 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     4501 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     4561 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     4621 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     4681 ctccaccggc ggcatggacg agctgtacaa ggcaacaaac ttctctctgc tgaaacaagc
     4741 cggagatgtc gaagagaatc ctggaccgat gaccgagtac aagcccacgg tgcgcctcgc
     4801 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     4861 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4921 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4981 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     5041 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     5101 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     5161 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     5221 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     5281 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     5341 gtgcatgacc cgcaagcccg gtgcctgatc ttatcgataa tcaacctctg gattacaaaa
     5401 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5461 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5521 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5581 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5641 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5701 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5761 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5821 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5881 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5941 ggatctccct ttgggccgcc tccccgcatc gataccgtcg accaaggcag ctgtagatct
     6001 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6061 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6121 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6181 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6241 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     6301 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     6361 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     6421 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     6481 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     6541 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     6601 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     6661 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     6721 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     6781 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctagct
     6841 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     6901 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6961 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7021 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7441 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8341 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     8401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9001 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     9061 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9121 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9301 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9361 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     9421 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     9481 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     9541 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     9601 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     9661 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     9721 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     9781 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     9841 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     9901 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     9961 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    10021 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    10081 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    10141 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    10201 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    10261 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    10321 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    10381 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    10441 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
    10501 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
    10561 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
    10621 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
    10681 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
    10741 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
    10801 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
    10861 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    10921 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    10981 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    11041 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    11101 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    11161 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    11221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11281 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    11341 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
    11401 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    11461 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
    11521 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
    11581 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
    11641 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
    11701 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
    11761 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
    11821 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
    11881 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
    11941 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    12001 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    12061 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    12121 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    12181 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    12241 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    12301 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    12361 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    12421 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    12481 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    12541 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    12601 accgcacagc aagcggccgg tgatcttcag acctggacga tatatatgag ggacaattgg
    12661 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    12721 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    12781 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    12841 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    12901 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    12961 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    13021 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    13081 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    13141 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    13201 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    13261 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    13321 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    13381 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    13441 agagacagag acagatccat tcgattagtg aacggatctc gacggtcg
//
