LOCUS       V030210                14187 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030210
VERSION     V030210
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3643
                     /label="CBLB(NM_001321786)"
                     /note="CBLB(NM_001321786)"
                     /gene="CBLB"
     CDS             3644..3670
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3716..4057
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4183..4682
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4703..5410
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5411..5467
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5468..6067
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6078..6666
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6729..6962
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6987..7005)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7794..7898
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7899..8759
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8930..9518
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9764..10093
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11227..11292
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11422..11442
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11867..12001
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12170..12803
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12850..12975
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13467..13700
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13885..13929
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttcattc ggcgcagtga
      601 actccaagcc accatgggct atttgtgtgt taatttcatt tggttcttgg gaataacgac
      661 tcaccgcgtt gatttaaaga aagaactaaa attccagatg gcaaactcaa tgaatggcag
      721 aaaccctggt ggtcgaggag gaaatccccg aaaaggtcga attttgggta ttattgatgc
      781 tattcaggat gcagttggac cccctaagca agctgccgca gatcgcagga ccgtggagaa
      841 gacttggaag ctcatggaca aagtggtaag actgtgccaa aatcccaaac ttcagttgaa
      901 aaatagccca ccatatatac ttgatatttt gcctgataca tatcagcatt tacgacttat
      961 attgagtaaa tatgatgaca accagaaact tgcccaactc agtgagaatg agtactttaa
     1021 aatctacatt gatagcctta tgaaaaagtc aaaacgggca ataagactct ttaaagaagg
     1081 caaggagaga atgtatgaag aacagtcaca ggacagacga aatctcacaa aactgtccct
     1141 tatcttcagt cacatgctgg cagaaatcaa agcaatcttt cccaatggtc aattccaggg
     1201 agataacttt cgtatcacaa aagcagatgc tgctgaattc tggagaaagt tttttggaga
     1261 caaaactatc gtaccatgga aagtattcag acagtgcctt catgaggtcc accagattag
     1321 ctctggcctg gaagcaatgg ctctaaaatc aacaattgat ttaacttgca atgattacat
     1381 ttcagttttt gaatttgata tttttaccag gctgtttcag ccttggggct ctattttgcg
     1441 gaattggaat ttcttagctg tgacacatcc aggttacatg gcatttctca catatgatga
     1501 agttaaagca cgactacaga aatatagcac caaacccgga agctatattt tccggttaag
     1561 ttgcactcga ttgggacagt gggccattgg ctatgtgact ggggatggga atatcttaca
     1621 gaccatacct cataacaagc ccttatttca agccctgatt gatggcagca gggaaggatt
     1681 ttatctttat cctgatggga ggagttataa tcctgattta actggattat gtgaacctac
     1741 acctcatgac catataaaag ttacacagga acaatatgaa ttatattgtg aaatgggctc
     1801 cacttttcag ctctgtaaga tttgtgcaga gaatgacaaa gatgtcaaga ttgagccttg
     1861 tgggcatttg atgtgcacct cttgccttac ggcatggcag gagtcggatg gtcagggctg
     1921 ccctttctgt cgttgtgaaa taaaaggaac tgagcccata atcgtggacc cctttgatcc
     1981 aagagatgaa ggctccaggt gttgcagcat cattgacccc tttggcatgc cgatgctaga
     2041 cttggacgac gatgatgatc gtgaggagtc cttgatgatg aatcggttgg caaacgtccg
     2101 aaagtgcact gacaggcaga actcaccagt cacatcacca ggatcctctc cccttgccca
     2161 gagaagaaag ccacagcctg acccactcca gatcccacat ctaagcctgc cacccgtgcc
     2221 tcctcgcctg gatctaattc agaaaggcat agttagatct ccctgtggca gcccaacggg
     2281 ttcaccaaag tcttctcctt gcatggtgag aaaacaagat aaaccactcc cagcaccacc
     2341 tcctccctta agagatcctc ctccaccgcc acctgaaaga cctccaccaa tcccaccaga
     2401 caatagactg agtagacaca tccatcatgt ggaaagcgtg ccttccagag acccgccaat
     2461 gcctcttgaa gcatggtgcc ctcgggatgt gtttgggact aatcagcttg tgggatgtcg
     2521 actcctaggg gagggctctc caaaacctgg aatcacagcg agttcaaatg tcaatggaag
     2581 gcacagtaga gtgggctctg acccagtgct tatgcggaaa cacagacgcc atgatttgcc
     2641 tttagaagga gctaaggtct tttccaatgg tcaccttgga agtgaagaat atgatgttcc
     2701 tccccggctt tctcctcctc ctccagttac caccctcctc cctagcataa agtgtactgg
     2761 tccgttagca aattctcttt cagagaaaac aagagaccca gtagaggaag atgatgatga
     2821 atacaagatt ccttcatccc accctgtttc cctgaattca caaccatctc attgtcataa
     2881 tgtaaaacct cctgttcggt cttgtgataa tggtcactgt atgctgaatg gaacacatgg
     2941 tccatcttca gagaagaaat caaacatccc tgacttaagc atatatttaa agggagatgt
     3001 ttttgattca gcctctgatc ccgtgccatt accacctgcc aggcctccaa ctcgggacaa
     3061 tccaaagcat ggttcttcac tcaacaggac gccctctgat tatgatcttc tcatccctcc
     3121 attaggtgaa gatgcttttg atgccctccc tccatctctc ccacctcccc cacctcctgc
     3181 aaggcatagt ctcattgaac attcaaaacc tcctggctcc agtagccggc catcctcagg
     3241 acaggatctt tttcttcttc cttcagatcc ctttgttgat ctagcaagtg gccaagttcc
     3301 tttgcctcct gctagaaggt taccaggtga aaatgtcaaa actaacagaa catcacagga
     3361 ctatgatcag cttccttcat gttcagatgg ttcacaggca ccagccagac cccctaaacc
     3421 acgaccgcgc aggactgcac cagaaattca ccacagaaaa ccccatgggc ctgaggcggc
     3481 attggaaaat gtcgatgcaa aaattgcaaa actcatggga gagggttatg cctttgaaga
     3541 ggtgaagaga gccttagaga tagcccagaa taatgtcgaa gttgcccgga gcatcctccg
     3601 agaatttgcc ttccctcctc cagtatcccc acgtctaaat ctatacccct acgacgtgcc
     3661 tgactacgcc ggcggcggtg gttcaggcgg tggcggttcc ggtggtgggg gatcagtcca
     3721 actggtggag tctggtggcg ctttggtgca gccaggtggc tctctgcgtt tgtcctgtgc
     3781 cgcttctggc ttcccagtga accgctattc catgcgctgg tatcgccagg ctccaggcaa
     3841 agagcgtgag tgggtagccg gtatgtccag cgcgggtgat cgtagctcct atgaagactc
     3901 cgtgaagggc cgtttcacca tcagccgtga cgatgcccgt aacacggtgt atctgcaaat
     3961 gaacagcttg aaacctgaag atacggccgt gtattactgt aatgtgaacg tgggcttcga
     4021 gtattggggc caaggcaccc aggtcaccgt ctccagctaa gatccttgac ttgcggccgc
     4081 aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcatc
     4141 taggcgccgg aattagatct ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     4201 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4261 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4321 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4381 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4441 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4501 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4561 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4621 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4681 cgacctgcag cccaagctta ccatggtgag caagggcgag gaggataaca tggccatcat
     4741 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     4801 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     4861 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     4921 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     4981 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5041 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     5101 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     5161 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     5221 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     5281 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     5341 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     5401 gctgtacaag gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     5461 tggaccgatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5521 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5581 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5641 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5701 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5761 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5821 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5881 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5941 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6001 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6061 tgcctgatct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6121 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6181 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6241 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6301 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6361 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6421 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6481 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6541 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6601 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6661 ccccgcatcg ataccgtcga ccaaggcagc tgtagatctt agccactttt taaaagaaaa
     6721 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6781 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6841 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6901 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6961 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     7021 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     7081 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     7141 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     7201 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     7261 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     7321 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     7381 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     7441 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     7501 ggagagcgtc gaatggtgca ctctcagtac aatctagctc tgatgccgca tagttaagcc
     7561 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7621 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7681 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7741 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9061 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9721 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     9781 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9841 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9901 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9961 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10021 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10081 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
    10141 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
    10201 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
    10261 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
    10321 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
    10381 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
    10441 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
    10501 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
    10561 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
    10621 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
    10681 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
    10741 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
    10801 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
    10861 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
    10921 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
    10981 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
    11041 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
    11101 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
    11161 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
    11221 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
    11281 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
    11341 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
    11401 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
    11461 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
    11521 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
    11581 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
    11641 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
    11701 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
    11761 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    11821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    11881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    12001 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    12061 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12121 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    12181 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    12241 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    12301 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    12361 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    12421 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    12481 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    12541 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    12601 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    12661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    12721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    12781 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    12841 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    12901 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    12961 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    13021 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    13081 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    13141 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    13201 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    13261 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggt
    13321 gatcttcaga cctggacgat atatatgagg gacaattgga gaagtgaatt atataaatat
    13381 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    13441 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    13501 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    13561 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    13621 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    13681 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    13741 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    13801 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    13861 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    13921 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    13981 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    14041 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    14101 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    14161 cgattagtga acggatctcg acggtcg
//
