LOCUS       V030188                13449 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030188
VERSION     V030188
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2905
                     /label="CUL2(NM_001198778)"
                     /note="CUL2(NM_001198778)"
                     /gene="CUL2"
     CDS             2906..2932
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2978..3319
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3445..3944
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3965..4672
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4673..4729
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4730..5329
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5340..5928
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5991..6224
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6249..6267)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7056..7160
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7161..8021
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8192..8780
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9026..9355
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10489..10554
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10684..10704
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11129..11263
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11432..12065
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12112..12237
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12729..12962
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13147..13191
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcttcat aacgcagtga
      601 actccaagcc accatgtaca gagtaacatg gtcaactttt tggcttagat ttcaacacta
      661 cacttgcaca atgtctttga aaccaagagt agtagatttt gatgaaacat ggaacaaact
      721 tttgacgaca ataaaagccg tggtcatgtt ggaatacgtc gaaagagcaa catggaatga
      781 ccgtttctca gatatctatg ctttatgtgt ggcctatcct gaaccccttg gagaaagact
      841 ttatacagaa actaagattt ttttggaaaa tcatgttcgg catttgcata agagagtttt
      901 ggagtcagaa gaacaagtac ttgttatgta tcataggtac tgggaagaat acagcaaggg
      961 tgcagactat atggactgct tatataggta tctcaacacc cagtttatta aaaagaataa
     1021 attaacagaa gcggaccttc agtatggcta tggtggtgta gatatgaatg aaccacttat
     1081 ggaaatagga gagctagcat tggatatgtg gaggaaattg atggttgaac cacttcaggc
     1141 catccttatc cgaatgctgc tccgagaaat caaaaatgat cgtggtggag aagacccaaa
     1201 ccagaaagta atccatgggg ttattaactc ctttgttcat gttgaacagt ataagaaaaa
     1261 attcccctta aagttttatc aggaaatttt tgagtctccc tttctgactg aaacaggaga
     1321 gtattacaaa caagaagctt caaatttatt acaagaatca aactgctcac agtatatgga
     1381 aaaggttcta ggtagattaa aagatgaaga aattcgatgt cgaaaatacc tacatccaag
     1441 ttcatatact aaggtgattc atgaatgtca acaacgaatg gtagcagacc acttacagtt
     1501 tttacatgca gaatgtcata atataattcg acaagagaaa aaaaatgaca tggcaaatat
     1561 gtacgtctta ctccgtgctg tgtccactgg tttacctcat atgattcagg agctgcaaaa
     1621 ccacatccat gatgagggcc ttcgagcaac cagcaacctt actcaggaaa acatgccaac
     1681 actatttgtg gagtcagttt tggaagtgca tggtaaattt gttcagctta tcaacactgt
     1741 tttgaatggt gatcagcatt ttatgagtgc gttggataag gcccttacgt cagttgtaaa
     1801 ttacagagaa cctaagtctg tttgcaaagc acctgaactg cttgctaagt actgtgacaa
     1861 cttactgaag aagtcagcga aagggatgac agagaatgaa gtggaagaca ggctcacgag
     1921 cttcatcaca gtgttcaaat acattgatga caaggacgtc tttcaaaagt tctacgcaag
     1981 aatgctggca aaacgtttaa ttcatgggtt atccatgtct atggactctg aagaagccat
     2041 gatcaacaaa ttaaagcaag cctgtggtta tgagtttacc agcaagctac atcggatgta
     2101 tacagatatg agtgtcagcg ctgatctcaa caataagttc aacaatttta tcaaaaacca
     2161 agacacagta atagatttgg gaattagttt tcaaatatat gttctacagg ctggtgcgtg
     2221 gcctcttact caggctcctt catctacgtt tgcaattccc caggaattag aaaaaagtgt
     2281 acagatgttt gaattatttt atagccaaca tttcagtgga aggaaactta catggttaca
     2341 ttatctgtgt acaggtgaag ttaaaatgaa ctatttgggc aaaccatatg tagccatggt
     2401 tacaacatac caaatggcag ttcttcttgc ctttaacaac agtgaaactg tcagttataa
     2461 agagcttcag gacagcactc agatgaatga aaaggaactg acaaaaacaa tcaaatcatt
     2521 acttgatgtg aaaatgatta accatgattc agaaaaggaa gatattgatg cagaatcttc
     2581 gttttcatta aatatgaact ttagcagtaa aagaacaaaa tttaaaatta ctacatcaat
     2641 gcagaaagac acaccacaag aaatggagca gactagaagt gcagttgatg aggaccggaa
     2701 aatgtatctc caagctgcta tagttcgtat catgaaagca cgaaaagtgc ttcggcacaa
     2761 tgcccttatt caagaggtga ttagccagtc aagagctagg tttaatccca gtatcagcat
     2821 gattaagaag tgtattgaag ttctgataga caaacaatac atagaacgca gccaggcgtc
     2881 ggcagatgaa tacagctacg tcgcgtaccc ctacgacgtg cctgactacg ccggcggcgg
     2941 tggttcaggc ggtggcggtt ccggtggtgg gggatcagtc caactggtgg agtctggtgg
     3001 cgctttggtg cagccaggtg gctctctgcg tttgtcctgt gccgcttctg gcttcccagt
     3061 gaaccgctat tccatgcgct ggtatcgcca ggctccaggc aaagagcgtg agtgggtagc
     3121 cggtatgtcc agcgcgggtg atcgtagctc ctatgaagac tccgtgaagg gccgtttcac
     3181 catcagccgt gacgatgccc gtaacacggt gtatctgcaa atgaacagct tgaaacctga
     3241 agatacggcc gtgtattact gtaatgtgaa cgtgggcttc gagtattggg gccaaggcac
     3301 ccaggtcacc gtctccagct aagatccttg acttgcggcc gcaactccca cctgcaacat
     3361 gcgtgactga ctgaggccgc gactctagag tcgacctgca tctaggcgcc ggaattagat
     3421 ctctcgaggt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     3481 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     3541 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     3601 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     3661 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     3721 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     3781 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3841 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3901 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3961 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     4021 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     4081 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     4141 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     4201 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     4261 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     4321 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     4381 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     4441 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4501 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4561 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4621 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcaacaaa
     4681 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgaccgagta
     4741 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4801 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4861 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4921 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4981 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5041 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5101 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5161 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5221 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5281 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat cttatcgata
     5341 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5401 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5461 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5521 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5581 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5641 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5701 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5761 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5821 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5881 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5941 gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6001 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6061 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6121 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6181 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     6241 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     6301 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     6361 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     6421 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     6481 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     6541 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     6601 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     6661 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     6721 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     6781 cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa
     6841 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6901 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6961 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7021 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8941 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9001 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9061 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9181 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9301 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9361 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     9421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     9481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9661 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     9721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     9781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     9841 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     9901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     9961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
    10021 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
    10081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
    10141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
    10201 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
    10261 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
    10321 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
    10381 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
    10441 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    10501 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    10561 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    10621 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    10681 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    10741 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    10801 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    10861 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    10921 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    10981 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    11041 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    11101 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    11161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    11221 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    11281 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt
    11341 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11401 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    11461 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    11521 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    11581 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    11641 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    11701 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11761 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11821 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11881 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11941 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    12001 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    12061 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    12121 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    12181 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    12241 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    12301 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    12361 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    12421 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    12481 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    12541 gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg
    12601 atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    12661 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    12721 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    12781 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    12841 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    12901 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    12961 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    13021 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    13081 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    13141 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    13201 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    13261 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    13321 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    13381 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    13441 cgacggtcg
//
