LOCUS       V033295                12909 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033295
VERSION     V033295
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2365
                     /label="IRF2BP1(NM_015649)"
                     /note="IRF2BP1(NM_015649)"
                     /gene="IRF2BP1"
     CDS             2366..2392
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2438..2779
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2905..3404
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3425..4132
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4133..4189
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4190..4789
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4800..5388
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5451..5684
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5709..5727)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6516..6620
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6621..7481
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7652..8240
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8486..8815
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9949..10014
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10144..10164
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10589..10723
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10892..11525
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11572..11697
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12189..12422
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12607..12651
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctagaatc catgcagtga
      601 actccaagcc accatggcgt ctgtgcaggc gtcccgccgc cagtggtgct acctgtgcga
      661 cctgcccaag atgccgtggg ccatggtgtg ggacttcagc gaggccgtgt gtcgcggctg
      721 cgtgaacttc gagggcgcgg accgcatcga actgctcatc gatgccgccc gccagctcaa
      781 gcgcagccac gtgctccccg agggccgctc gcccgggccc ccggccctta agcacccggc
      841 caccaaggac ctggcggcgg cagccgcaca ggggccccag ctgccgcccc cgcaggccca
      901 gccccagccg tcagggaccg gcggcggcgt gtcgggccag gaccgctatg acagggccac
      961 atcatcaggc cgcctccccc tgccctcgcc cgccctggag tacactctgg ggtcccgcct
     1021 ggccaatggg ctgggccgtg aggaggccgt ggctgagggg gcgcgaaggg ccttgcttgg
     1081 ctccatgcct ggcttgatgc cccctgggct gctggcagct gcagtgtctg gcctgggaag
     1141 ccgaggcctg acgctggcac ccggcttgag tcctgcccgt cccctcttcg gctccgattt
     1201 cgagaaagag aagcagcaga ggaatgcgga ctgtctggca gaactgaacg aggccatgcg
     1261 aggccgggca gaggaatggc acgggcgccc caaagcagtg cgggaacagc tactggcgct
     1321 gtccgcctgc gccccgttca atgtgcgctt caagaaggat cacgggctgg tggggcgagt
     1381 gttcgccttc gatgctactg cccgtcctcc aggatacgag ttcgagctga agctcttcac
     1441 cgaatacccc tgtggttccg gcaatgtgta cgccggcgtc ctggcagtgg ctcgccagat
     1501 gttccacgat gctctgcggg agccgggcaa ggcactggct tcttcgggct tcaagtacct
     1561 cgaatatgaa cgccggcatg gatcaggaga atggcggcag ctgggcgagc tgcttaccga
     1621 cggcgtccgc agcttccgcg agccagctcc cgcggaggcc ctgccccagc agtacccaga
     1681 gccggcccct gcggctctct gtggcccacc cccgcgagcc ccatcccgga acctggcgcc
     1741 cacgccgcgc cgtcgcaagg catcccccga gccggagggc gaggcggctg ggaagatgac
     1801 caccgaggag cagcagcaac ggcactgggt ggcacccggc ggcccgtact ccgctgagac
     1861 ccctggtgtg ccctcgccca ttgccgccct gaagaatgtg gccgaagccc tgggccactc
     1921 acccaaggac cctggcggag gcggggggcc tgtgcgtgca gggggcgcca gccctgcagc
     1981 ctcctccacg gcccagccgc caacccagca tcgccttgtg gcccgcaacg gcgaagcaga
     2041 agtcagtccc acagcggggg ccgaagctgt cagcgggggt ggcagcggca ctggggcgac
     2101 ccctggggcc cccctgtgct gtaccctgtg cagggagcgg ctagaagaca cccacttcgt
     2161 ccagtgcccc tcggtgcccg gacacaagtt ctgctttccc tgctcccggg agttcatcaa
     2221 ggcgcagggc ccggccgggg aggtgtactg cccgagcgga gacaagtgcc cgctggtcgg
     2281 ctcctccgtg ccctgggcct tcatgcaggg cgagatcgcc accatccttg ctggagacat
     2341 caaagttaag aaagaacggg acccctaccc ctacgacgtg cctgactacg ccggcggcgg
     2401 tggttcaggc ggtggcggtt ccggtggtgg gggatcagtc caactggtgg agtctggtgg
     2461 cgctttggtg cagccaggtg gctctctgcg tttgtcctgt gccgcttctg gcttcccagt
     2521 gaaccgctat tccatgcgct ggtatcgcca ggctccaggc aaagagcgtg agtgggtagc
     2581 cggtatgtcc agcgcgggtg atcgtagctc ctatgaagac tccgtgaagg gccgtttcac
     2641 catcagccgt gacgatgccc gtaacacggt gtatctgcaa atgaacagct tgaaacctga
     2701 agatacggcc gtgtattact gtaatgtgaa cgtgggcttc gagtattggg gccaaggcac
     2761 ccaggtcacc gtctccagct aagatccttg acttgcggcc gcaactccca cctgcaacat
     2821 gcgtgactga ctgaggccgc gactctagag tcgacctgca tctaggcgcc ggaattagat
     2881 ctctcgaggt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     2941 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     3001 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     3061 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     3121 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     3181 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     3241 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3301 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3361 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3421 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3481 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3541 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3601 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3661 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3721 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3781 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3841 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3901 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     3961 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4021 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4081 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcaacaaa
     4141 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgaccgagta
     4201 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4261 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4321 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4381 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4441 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4501 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     4561 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     4621 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     4681 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     4741 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat cttatcgata
     4801 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4861 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4921 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4981 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5041 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5101 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5161 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5221 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5281 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5341 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5401 gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5461 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5521 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5581 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5641 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     5701 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     5761 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     5821 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     5881 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     5941 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     6001 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     6061 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     6121 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     6181 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     6241 cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa
     6301 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6361 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6421 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6481 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6541 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6601 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6661 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6721 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6781 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6841 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6901 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6961 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7021 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7081 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7141 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7201 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7261 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7321 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7381 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7441 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7501 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7561 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7621 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7681 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7741 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7801 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7861 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7921 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7981 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8041 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8101 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8161 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8221 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8281 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8341 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8401 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8461 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8521 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8581 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8641 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8701 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8761 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8821 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     8881 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     8941 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9001 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9061 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9121 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     9181 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     9241 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     9301 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     9361 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     9421 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     9481 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     9541 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     9601 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     9661 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     9721 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     9781 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
     9841 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
     9901 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     9961 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    10021 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    10081 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    10141 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    10201 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    10261 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    10321 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    10381 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    10441 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    10501 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10561 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10681 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10741 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt
    10801 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10861 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    10921 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    10981 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    11041 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    11101 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    11161 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11221 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11281 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11341 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11401 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11461 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11521 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    11581 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    11641 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    11701 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    11761 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    11821 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    11881 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    11941 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    12001 gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg
    12061 atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    12121 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    12181 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    12241 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    12301 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    12361 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    12421 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    12481 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    12541 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    12601 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    12661 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    12721 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    12781 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    12841 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    12901 cgacggtcg
//
