LOCUS       V030214                13209 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030214
VERSION     V030214
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2665
                     /label="KBTBD7(NM_032138)"
                     /note="KBTBD7(NM_032138)"
                     /gene="KBTBD7"
     CDS             2666..2692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2738..3079
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3205..3704
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3725..4432
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4433..4489
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4490..5089
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5100..5688
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5751..5984
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6009..6027)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6816..6920
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6921..7781
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7952..8540
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8786..9115
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10249..10314
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10444..10464
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10889..11023
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11192..11825
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11872..11997
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12489..12722
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12907..12951
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcacgcg gcagcagtga
      601 actccaagcc accatgcagt cccgggaaga cgtcccgcgc tctcgccgcc tcgccagtcc
      661 ccgtggtggg aggcggccca agaggatttc caagccctcg gtttcggcct ttttcacggg
      721 tccagaggag ttaaaggaca cggcccattc tgcagccctg ctggcacagc tcaagtcctt
      781 ctacgacgcg cggctgctgt gtgatgtgac catcgaggtg gtgacgcctg gcagcgggcc
      841 tggcacgggt cgcctctttt cctgcaatcg caacgtgcta gcagctgcgt gtccctactt
      901 caagagcatg ttcacaggtg gcatgtacga gagccagcag gccagcgtga ccatgcacga
      961 tgtggacgcc gagtccttcg aggtgttggt cgactactgc tacacgggtc gtgtgtctct
     1021 cagtgaggcc aatgtgcagc gcctgtacgc ggcctccgac atgctacagc tggaatatgt
     1081 gcgggaagcc tgtgcctcct tcttagcccg acgtcttgac ctgaccaact gcaccgccat
     1141 cctcaagttt gcagacgcct tcgaccatca caagcttcga tctcaggccc agtcctacat
     1201 agctcacaac ttcaagcagc tcagccgaat gggttcaatt cgggaggaga ctctagcaga
     1261 tctaaccctg gcccagctgc tggctgtcct acgcctggat agtctggaca tagagagtga
     1321 gcggactgta tgccatgtag ctgtgcagtg gctggaggct gctgccaaag agcggggtcc
     1381 cagtgctgca gaagtcttca agtgcgtgcg ctggatgcac ttcactgaag aagatcagga
     1441 ctacttagaa gggctgctga ccaagcccat cgtgaagaag tactgcctgg acgttattga
     1501 aggggccctg cagatgcgct atggtgacct gttgtacaag tctctggtgc cagtgccaaa
     1561 cagcagcagc agcagtagca gcagcaactc tcttgtatct gcagcagaaa atccacccca
     1621 gagactgggt atgtgtgcca aggagatggt gatcttcttt ggacatccta gagatccctt
     1681 tctctgctat gacccttact cgggggacat ttacacaatg ccatcccctt tgaccagctt
     1741 tgctcacact aagactgtca cctcctcagc tgtctgtgtg tccccagacc atgacatcta
     1801 tctagctgct cagcccagga aagacctctg ggtgtataaa ccagctcaga atagttggca
     1861 gcaacttgca gatcgcttgc tgtgtcgtga gggcatggat gtggcatatc tcaatggcta
     1921 catctacatt ttggggggac gagaccctat tactggagtt aagttgaagg aagtggaatg
     1981 ctacagtgtt cagagaaacc agtgggcatt ggtggctcct gtccctcatt ccttctattc
     2041 ctttgaactc atagtggttc agaactatct ttatgctgtc aacagtaagc gcatgctttg
     2101 ctatgatcct agccacaata tgtggctgaa ctgtgcttct cttaaacgta gtgactttca
     2161 ggaagcatgt gtcttcaatg atgaaatcta ttgtatctgt gacatcccag tcatgaaggt
     2221 ctacaaccca gctaggggag aatggaggcg gattagtaat attcctttgg attcagagac
     2281 ccacaactac cagattgtca atcatgacca aaagttgctt ctcatcactt ctacaacccc
     2341 acaatggaaa aagaaccgag tgacagtgta tgagtatgat actagggaag atcagtggat
     2401 taatataggt accatgttag gccttttgca gtttgactct ggctttattt gcctttgtgc
     2461 tcgtgtttat ccttcctgcc ttgaacctgg tcagagtttt attactgagg aagatgatgc
     2521 acggagtgag tctagtactg aatgggactt agatggattc agtgagctgg actctgagtc
     2581 aggaagttca agttcttttt cagatgatga agtctgggtg caagtagcac ctcagcgaaa
     2641 tgcacaggat cagcagggtt ctttgtaccc ctacgacgtg cctgactacg ccggcggcgg
     2701 tggttcaggc ggtggcggtt ccggtggtgg gggatcagtc caactggtgg agtctggtgg
     2761 cgctttggtg cagccaggtg gctctctgcg tttgtcctgt gccgcttctg gcttcccagt
     2821 gaaccgctat tccatgcgct ggtatcgcca ggctccaggc aaagagcgtg agtgggtagc
     2881 cggtatgtcc agcgcgggtg atcgtagctc ctatgaagac tccgtgaagg gccgtttcac
     2941 catcagccgt gacgatgccc gtaacacggt gtatctgcaa atgaacagct tgaaacctga
     3001 agatacggcc gtgtattact gtaatgtgaa cgtgggcttc gagtattggg gccaaggcac
     3061 ccaggtcacc gtctccagct aagatccttg acttgcggcc gcaactccca cctgcaacat
     3121 gcgtgactga ctgaggccgc gactctagag tcgacctgca tctaggcgcc ggaattagat
     3181 ctctcgaggt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     3241 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     3301 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     3361 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     3421 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     3481 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     3541 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3601 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3661 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3721 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3781 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3841 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3901 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3961 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     4021 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     4081 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     4141 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     4201 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4261 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4321 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4381 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcaacaaa
     4441 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgaccgagta
     4501 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4561 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4621 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4681 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4741 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4801 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     4861 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     4921 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     4981 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5041 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat cttatcgata
     5101 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5161 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5221 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5281 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5341 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5401 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5461 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5521 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5581 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5641 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5701 gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5761 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5821 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5881 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5941 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     6001 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     6061 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     6121 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     6181 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     6241 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     6301 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     6361 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     6421 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     6481 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     6541 cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa
     6601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8761 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8941 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9001 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9061 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9121 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     9181 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     9241 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9301 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9361 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9421 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     9481 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     9541 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     9601 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     9661 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     9721 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     9781 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     9841 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     9901 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     9961 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
    10021 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
    10081 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
    10141 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
    10201 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    10261 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    10321 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    10381 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    10441 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    10501 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    10561 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    10621 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    10681 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    10741 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    10801 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10861 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10921 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10981 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    11041 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt
    11101 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11161 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    11221 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    11281 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    11341 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    11401 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    11461 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11521 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11581 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11641 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11701 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11761 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11821 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    11881 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    11941 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    12001 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    12061 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    12121 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    12181 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    12241 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    12301 gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg
    12361 atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    12421 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    12481 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    12541 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    12601 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    12661 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    12721 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    12781 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    12841 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    12901 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    12961 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    13021 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    13081 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    13141 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    13201 cgacggtcg
//
