LOCUS       V033661                12654 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033661
VERSION     V033661
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2110
                     /label="KCNA2(NM_004974)"
                     /note="KCNA2(NM_004974)"
                     /gene="KCNA2"
     CDS             2111..2137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2183..2524
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2650..3149
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3170..3877
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3878..3934
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3935..4534
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4545..5133
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5196..5429
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5454..5472)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6261..6365
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6366..7226
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7397..7985
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8231..8560
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9694..9759
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9889..9909
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10334..10468
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10637..11270
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11317..11442
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11934..12167
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12352..12396
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctctttcg aaagcagtga
      601 actccaagcc accatgacag tggccaccgg agacccagca gacgaggctg ctgccctccc
      661 tgggcaccca caggacacct atgacccaga ggcagaccac gagtgctgtg agagggtggt
      721 gatcaacatc tcagggctgc ggtttgagac ccagctaaag accttagccc agtttccaga
      781 gaccctctta ggggacccaa agaaacgaat gaggtacttt gaccccctcc gaaatgagta
      841 ctttttcgat cggaaccgcc ctagctttga tgccattttg tactactacc agtcaggggg
      901 ccgattgagg cgacctgtga atgtgccctt agatatattc tctgaagaaa ttcggtttta
      961 tgagctggga gaagaagcga tggagatgtt tcgggaagat gaaggctaca tcaaggagga
     1021 agagcgtcct ctgcctgaaa atgagtttca gagacaagtg tggcttctct ttgaataccc
     1081 agagagctca gggcctgcca ggattatagc tattgtgtct gtcatggtga ttctgatctc
     1141 aattgtcagc ttctgtctgg aaacattgcc catcttccgg gatgagaatg aagacatgca
     1201 tggtagtggg gtgaccttcc acacctattc caacagcacc atcgggtacc agcagtccac
     1261 ttccttcaca gaccctttct tcattgtaga gacactctgc atcatctggt tctcctttga
     1321 attcttggtg aggttctttg cctgtcccag caaagccggc ttcttcacca acatcatgaa
     1381 catcattgac attgtggcca tcatccccta cttcatcacc ctggggacag agttggctga
     1441 gaagccagag gacgctcagc aaggccagca ggccatgtca ctggccatcc tccgtgtcat
     1501 ccggttggta agagtcttta ggattttcaa gttgtccaga cactccaaag gtctccagat
     1561 tctaggtcag accctcaaag ccagcatgag agaattgggc ctcctgatat tctttctctt
     1621 cataggggtc atccttttct ctagtgctgt gtattttgca gaggccgatg agcgagagtc
     1681 ccagttcccc agcatcccag atgccttctg gtgggcagtc gtctccatga caactgtagg
     1741 ctatggagac atggttccga ctaccattgg gggaaagata gtgggttccc tatgtgcgat
     1801 tgcaggtgtg ttaactattg ccttaccggt ccctgtcatt gtgtccaatt tcaactactt
     1861 ctaccaccgg gagacagagg gagaggaaca ggcccaatac ttgcaagtga caagctgtcc
     1921 aaagatccca tcctcccctg acctaaagaa aagtagaagt gcctctacca ttagtaagtc
     1981 tgattacatg gagatccagg agggtgtaaa taacagtaat gaggacttta gagaggaaaa
     2041 cttgaaaaca gccaactgta ccttggctaa cacaaactat gtgaatatta ccaaaatgtt
     2101 aactgatgtc tacccctacg acgtgcctga ctacgccggc ggcggtggtt caggcggtgg
     2161 cggttccggt ggtgggggat cagtccaact ggtggagtct ggtggcgctt tggtgcagcc
     2221 aggtggctct ctgcgtttgt cctgtgccgc ttctggcttc ccagtgaacc gctattccat
     2281 gcgctggtat cgccaggctc caggcaaaga gcgtgagtgg gtagccggta tgtccagcgc
     2341 gggtgatcgt agctcctatg aagactccgt gaagggccgt ttcaccatca gccgtgacga
     2401 tgcccgtaac acggtgtatc tgcaaatgaa cagcttgaaa cctgaagata cggccgtgta
     2461 ttactgtaat gtgaacgtgg gcttcgagta ttggggccaa ggcacccagg tcaccgtctc
     2521 cagctaagat ccttgacttg cggccgcaac tcccacctgc aacatgcgtg actgactgag
     2581 gccgcgactc tagagtcgac ctgcatctag gcgccggaat tagatctctc gaggttaacg
     2641 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     2701 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     2761 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     2821 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     2881 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     2941 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     3001 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     3061 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     3121 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tggtgagcaa
     3181 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     3241 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3301 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     3361 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3421 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3481 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     3541 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3601 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     3661 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     3721 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     3781 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     3841 ccgccactcc accggcggca tggacgagct gtacaaggca acaaacttct ctctgctgaa
     3901 acaagccgga gatgtcgaag agaatcctgg accgatgacc gagtacaagc ccacggtgcg
     3961 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4021 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4081 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4141 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4201 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4261 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4321 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4381 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4441 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4501 cacctggtgc atgacccgca agcccggtgc ctgatcttat cgataatcaa cctctggatt
     4561 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4621 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4681 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4741 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4801 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4861 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4921 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4981 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5041 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5101 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacca aggcagctgt
     5161 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5221 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5281 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5341 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5401 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     5461 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     5521 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     5581 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     5641 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     5701 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     5761 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     5821 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     5881 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     5941 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     6001 ctagctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6061 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6121 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     6181 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6241 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6301 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6361 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6421 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6481 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6541 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6601 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6661 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6721 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6781 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6841 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6901 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6961 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7021 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7081 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7141 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7201 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7261 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7321 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7981 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8041 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8101 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8161 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8221 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8281 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8341 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8401 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8461 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8521 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     8581 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     8641 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     8701 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     8761 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     8821 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     8881 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     8941 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     9001 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     9061 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     9121 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     9181 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     9241 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     9301 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     9361 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     9421 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     9481 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     9541 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     9601 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     9661 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     9721 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     9781 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     9841 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     9901 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     9961 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
    10021 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
    10081 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
    10141 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
    10201 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
    10261 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    10321 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10381 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10441 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    10501 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
    10561 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    10621 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
    10681 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
    10741 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
    10801 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
    10861 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
    10921 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
    10981 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
    11041 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
    11101 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    11161 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    11221 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    11281 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    11341 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    11401 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    11461 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    11521 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    11581 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    11641 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    11701 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    11761 aagaccaccg cacagcaagc ggccggtgat cttcagacct ggacgatata tatgagggac
    11821 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    11881 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    11941 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    12001 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    12061 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    12121 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    12181 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    12241 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    12301 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    12361 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    12421 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    12481 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    12541 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    12601 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtcg
//
