LOCUS       V033275                12657 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033275
VERSION     V033275
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2113
                     /label="KCNV1(NM_014379)"
                     /note="KCNV1(NM_014379)"
                     /gene="KCNV1"
     CDS             2114..2140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2186..2527
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2653..3152
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3173..3880
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3881..3937
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3938..4537
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4548..5136
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5199..5432
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5457..5475)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6264..6368
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6369..7229
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7400..7988
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8234..8563
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9697..9762
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9892..9912
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10337..10471
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10640..11273
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11320..11445
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11937..12170
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12355..12399
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctctcagt gccgcagtga
      601 actccaagcc accatgcctt ccagcggcag agcgctgctg gactcgccgc tggacagcgg
      661 ctccctgacc tccctggact ctagtgtctt ctgcagcgag ggtgaagggg agcccttggc
      721 gctcggggac tgcttcacgg tcaacgtggg cggcagccgc ttcgtgctct cgcagcaggc
      781 gctgtcctgc ttcccgcaca cgcgccttgg caagctggcc gtggtggtgg cttcctaccg
      841 ccgccccggg gccctggccg ccgtgcccag ccctctggag ctttgcgacg atgccaaccc
      901 cgtggacaac gagtacttct tcgaccgcag ctcgcaggcg ttccgatatg tcctgcacta
      961 ctaccgcacc ggccgcctgc atgtcatgga gcagctgtgc gcgctctcct tcctgcagga
     1021 gatccagtac tggggcatcg atgagctcag catcgattcc tgctgcaggg acagatactt
     1081 cagaaggaaa gagctgagtg aaactttaga cttcaagaag gacacagaag accaggaaag
     1141 tcaacatgag agtgaacagg acttctccca aggaccttgt cccactgttc gccagaagct
     1201 ctggaatatc ctggagaaac ctggatcttc cacagctgcc cgtatctttg gcgtcatctc
     1261 cattatcttc gtggtggtgt ccatcattaa catggccctg atgtcagctg agttaagctg
     1321 gctggacctg cagctgctgg aaatcctgga gtatgtgtgc attagctggt tcaccgggga
     1381 gtttgtcctc cgcttcctgt gtgtgcggga caggtgtcgc ttcctaagaa aggtgccaaa
     1441 catcatagac ctccttgcca tcttgccctt ctacatcact cttctggtag agagcctaag
     1501 tgggagccag accacgcagg agctggagaa cgtggggcgc attgtccagg tgttgaggct
     1561 gctcagggct ctgcgcatgc taaagctggg cagacattcc acaggattac gctcccttgg
     1621 gatgacaatc acccagtgtt acgaagaagt cggcctactg ctcctatttc tatccgtggg
     1681 aatctctata ttttcaactg tagaatactt tgctgagcaa agcattcctg acacaacctt
     1741 cacaagtgtc ccttgtgcat ggtggtgggc caccacctct atgactactg tgggatatgg
     1801 ggacattaga ccagacacca ccacaggcaa aatcgtggcc ttcatgtgta tattatcggg
     1861 aattcttgtc ttggccttgc ctattgctat tattaacgat cgcttctctg cttgctactt
     1921 caccttgaaa ctcaaggaag cagctgttag acagcgtgaa gccctaaaga agcttaccaa
     1981 gaatatagcc actgactcat atatcagtgt taacttgaga gatgtctatg cccggagtat
     2041 catggagatg ctgcgactga aaggcagaga aagagcaagt actaggagca gcgggggaga
     2101 tgatttctgg ttttacccct acgacgtgcc tgactacgcc ggcggcggtg gttcaggcgg
     2161 tggcggttcc ggtggtgggg gatcagtcca actggtggag tctggtggcg ctttggtgca
     2221 gccaggtggc tctctgcgtt tgtcctgtgc cgcttctggc ttcccagtga accgctattc
     2281 catgcgctgg tatcgccagg ctccaggcaa agagcgtgag tgggtagccg gtatgtccag
     2341 cgcgggtgat cgtagctcct atgaagactc cgtgaagggc cgtttcacca tcagccgtga
     2401 cgatgcccgt aacacggtgt atctgcaaat gaacagcttg aaacctgaag atacggccgt
     2461 gtattactgt aatgtgaacg tgggcttcga gtattggggc caaggcaccc aggtcaccgt
     2521 ctccagctaa gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact
     2581 gaggccgcga ctctagagtc gacctgcatc taggcgccgg aattagatct ctcgaggtta
     2641 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     2701 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     2761 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     2821 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     2881 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     2941 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     3001 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     3061 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     3121 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatggtgag
     3181 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     3241 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3301 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     3361 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     3421 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3481 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     3541 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3601 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     3661 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     3721 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     3781 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     3841 gggccgccac tccaccggcg gcatggacga gctgtacaag gcaacaaact tctctctgct
     3901 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg accgagtaca agcccacggt
     3961 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4021 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4081 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4141 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4201 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4261 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4321 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4381 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4441 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4501 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct tatcgataat caacctctgg
     4561 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4621 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4681 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4741 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4801 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4861 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4921 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     4981 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5041 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5101 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga ccaaggcagc
     5161 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5221 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5281 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5341 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5401 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     5461 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     5521 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     5581 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     5641 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     5701 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     5761 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     5821 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     5881 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     5941 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     6001 aatctagctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     6061 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6121 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     6181 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6241 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6301 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6361 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6421 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6481 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6541 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6601 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6661 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6721 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6781 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6841 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6901 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6961 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7021 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7081 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7141 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7201 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7261 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7321 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7381 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7441 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7501 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7561 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7621 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7681 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7741 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7801 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7861 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7921 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7981 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8041 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8101 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8161 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8221 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     8281 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8341 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8401 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8461 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8521 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     8581 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     8641 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     8701 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     8761 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     8821 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     8881 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     8941 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     9001 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     9061 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     9121 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     9181 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     9241 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     9301 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     9361 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     9421 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     9481 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     9541 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     9601 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     9661 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     9721 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     9781 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     9841 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     9901 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     9961 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
    10021 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
    10081 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
    10141 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
    10201 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
    10261 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    10321 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10381 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10441 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10501 tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg tttcatttac
    10561 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10621 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    10681 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    10741 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    10801 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    10861 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    10921 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    10981 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    11041 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    11101 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    11161 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    11221 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    11281 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    11341 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    11401 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    11461 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    11521 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    11581 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    11641 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    11701 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    11761 agtaagacca ccgcacagca agcggccggt gatcttcaga cctggacgat atatatgagg
    11821 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11881 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11941 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    12001 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    12061 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    12121 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    12181 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    12241 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    12301 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    12361 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    12421 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    12481 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    12541 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    12601 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtcg
//
