LOCUS       V033283                12672 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033283
VERSION     V033283
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2128
                     /label="RABGEF1(NM_001287061)"
                     /note="RABGEF1(NM_001287061)"
                     /gene="RABGEF1"
     CDS             2129..2155
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2201..2542
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2668..3167
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3188..3895
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3896..3952
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3953..4552
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4563..5151
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5214..5447
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5472..5490)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6279..6383
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6384..7244
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7415..8003
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8249..8578
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9712..9777
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9907..9927
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10352..10486
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10655..11288
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11335..11460
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11952..12185
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12370..12414
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctaaacct cgggcagtga
      601 actccaagcc accatgatgg cgtcttcata ccatgaagtg gttagcagga agaagatgag
      661 ccttaagtct gaacgccgag gaattcatgt ggatcaatcg gatctcctgt gcaagaaagg
      721 atgtggttac tacggcaacc ctgcctggca gggtttctgc tccaagtgct ggagggaaga
      781 gtaccacaaa gccaggcaga agcagattca ggaggactgg gagctggcgg agcgactcca
      841 gcgggaggaa gaagaggcct ttgccagcag tcagagcagc caaggggccc aatccctcac
      901 attctccaag tttgaagaaa agaaaaccaa cgagaagacc cgcaaggtta ccacagtgaa
      961 gaaattcttc agtgcatctt ccagggtcgg atcaaagaag gaaattcagg aagcaaaagc
     1021 tcccagtcct tccataaacc ggcaaaccag cattgaaacg gatagagtgt ctaaggagtt
     1081 catagaattt ctcaagacct tccacaagac aggccaagaa atctataaac agaccaagct
     1141 gtttttggaa ggaatgcatt acaaaaggga tctaagcatt gaagaacagt cagagtgtgc
     1201 tcaggatttc taccacaatg tggccgaaag gatgcaaact cgtgggaaag tgcctccaga
     1261 aagagtcgag aagataatgg atcagattga aaagtacatc atgactcgtc tctataaata
     1321 tgtattctgt ccagaaacta ctgatgatga gaagaaagat cttgccattc aaaagagaat
     1381 cagagccctg cgctgggtta cgcctcagat gctgtgtgtc cctgttaatg aagacatccc
     1441 agaagtgtct gatatggtgg tgaaggcgat cacagatatc attgaaatgg attccaagcg
     1501 tgtgcctcga gacaagctgg cctgcatcac caagtgcagc aagcacatct tcaatgccat
     1561 caagatcacc aagaatgagc cggcgtcagc ggatgacttc ctccccaccc tcatctacat
     1621 tgttttgaag ggcaaccccc cacgccttca gtctaatatc cagtatatca cgcgcttctg
     1681 caatccaagc cgactgatga ctggagagga tggctactat ttcaccaatc tgtgctgtgc
     1741 tgtggctttc attgagaagc tagacgccca gtctttgaat ctaagtcagg aggattttga
     1801 tcgctacatg tctggccaga cctctcccag gaagcaagaa gctgagagtt ggtctcctga
     1861 tgcttgctta ggcgtcaagc aaatgtataa gaacttggat ctcttgtctc agttgaatga
     1921 acgacaagaa aggatcatga atgaagccaa gaaactggaa aaagacctca tagattggac
     1981 agatggaatt gcaagagaag ttcaagacat cgttgagaaa tacccactgg aaattaagcc
     2041 tccgaatcaa ccgttagcag ctattgactc tgaaaacgtt gaaaatgata aacttcctcc
     2101 accactgcaa cctcaagttt atgcaggata cccctacgac gtgcctgact acgccggcgg
     2161 cggtggttca ggcggtggcg gttccggtgg tgggggatca gtccaactgg tggagtctgg
     2221 tggcgctttg gtgcagccag gtggctctct gcgtttgtcc tgtgccgctt ctggcttccc
     2281 agtgaaccgc tattccatgc gctggtatcg ccaggctcca ggcaaagagc gtgagtgggt
     2341 agccggtatg tccagcgcgg gtgatcgtag ctcctatgaa gactccgtga agggccgttt
     2401 caccatcagc cgtgacgatg cccgtaacac ggtgtatctg caaatgaaca gcttgaaacc
     2461 tgaagatacg gccgtgtatt actgtaatgt gaacgtgggc ttcgagtatt ggggccaagg
     2521 cacccaggtc accgtctcca gctaagatcc ttgacttgcg gccgcaactc ccacctgcaa
     2581 catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcatctaggc gccggaatta
     2641 gatctctcga ggttaacgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     2701 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     2761 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     2821 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     2881 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     2941 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3001 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     3061 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3121 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     3181 gcttaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     3241 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     3301 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     3361 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     3421 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     3481 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     3541 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     3601 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     3661 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     3721 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     3781 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     3841 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggcaac
     3901 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatgaccga
     3961 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     4021 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     4081 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     4141 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     4201 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     4261 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     4321 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     4381 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     4441 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     4501 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatcttatcg
     4561 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4621 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4681 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4741 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4801 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4861 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4921 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4981 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5041 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5101 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     5161 gtcgaccaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5221 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5281 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5341 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5401 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
     5461 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     5521 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
     5581 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
     5641 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
     5701 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     5761 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     5821 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     5881 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     5941 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     6001 gtgcactctc agtacaatct agctctgatg ccgcatagtt aagccagccc cgacacccgc
     6061 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6121 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6181 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     6241 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6301 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6361 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6421 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6481 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6541 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6601 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6661 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6721 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6781 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6841 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6901 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6961 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7021 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7081 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7141 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7201 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7261 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7321 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7381 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7441 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7501 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7561 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7621 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7681 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7741 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7801 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7861 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7921 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7981 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8041 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8101 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8161 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8221 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8341 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8401 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8461 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8521 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8581 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     8641 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     8701 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     8761 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     8821 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     8881 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     8941 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     9001 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     9061 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     9121 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     9181 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     9241 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     9301 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     9361 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     9421 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     9481 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     9541 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     9601 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     9661 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     9721 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     9781 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     9841 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     9901 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     9961 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
    10021 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
    10081 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
    10141 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    10201 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    10261 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    10321 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    10381 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10441 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10501 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10561 agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    10621 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
    10681 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
    10741 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
    10801 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
    10861 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
    10921 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
    10981 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
    11041 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
    11101 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    11161 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    11221 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    11281 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
    11341 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    11401 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    11461 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    11521 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    11581 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    11641 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    11701 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    11761 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggtgatct tcagacctgg
    11821 acgatatata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    11881 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    11941 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    12001 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    12061 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    12121 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    12181 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    12241 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    12301 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    12361 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    12421 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    12481 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    12541 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    12601 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    12661 tctcgacggt cg
//
