LOCUS       V033230                13029 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033230
VERSION     V033230
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2485
                     /label="RLIM(NM_016120)"
                     /note="RLIM(NM_016120)"
                     /gene="RLIM"
     CDS             2486..2512
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2558..2899
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3025..3524
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3545..4252
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4253..4309
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4310..4909
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4920..5508
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5571..5804
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5829..5847)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6636..6740
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6741..7601
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7772..8360
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8606..8935
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10069..10134
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10264..10284
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10709..10843
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11012..11645
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11692..11817
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12309..12542
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12727..12771
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttccgca ggtgcagtga
      601 actccaagcc accatggaaa actcagattc caatgacaaa ggaagtggtg atcagtctgc
      661 agcacagcgc agaagtcaga tggaccgatt ggatcgagaa gaagctttct atcaatttgt
      721 aaataacctg agtgaagaag attataggct tatgagagat aacaatttgc taggcacccc
      781 aggtgaaagt actgaggaag agttgctgag acgactacag caaattaaag aaggcccacc
      841 accgcaaaac tcagatgaaa atagaggagg agactcttca gatgatgtgt ctaatggtga
      901 ctctataata gactggctta actctgtcag acaaactgga aatacaacaa gaagtgggca
      961 aagaggaaac caatcttgga gagcagtgag tcggactaat ccaaacagtg gtgatttcag
     1021 attcagttta gagataaatg ttaaccgtaa taatgggagc caaaattcag agaatgaaaa
     1081 tgagccatct gcaagacgtt ctagtggaga aaatgtggaa aacaacagcc aaaggcaagt
     1141 ggaaaaccca cgatctgaat caacatctgc aaggccatct agatcagaac gaaattcaac
     1201 tgaagcgtta acagaggtcc cacctaccag aggtcagagg agggcaagaa gcaggagccc
     1261 agaccatcgg agaaccagag caagagctga aagaagtagg tcacctctgc atccaatgag
     1321 tgaaattcca cgaagatctc atcatagtat ctcatctcag acttttgaac atcctttggt
     1381 aaatgagacg gagggaagtt ctagaacccg gcaccatgtg acattgaggc agcaaatatc
     1441 tgggcctgag ttgctaagta gaggtctttt tgcagcttct ggaacaagaa atgcttctca
     1501 aggagcaggt tcttcagaca cagctgccag tggtgaatct acaggatcag gacagagacc
     1561 tccaaccata gtccttgatc ttcaagtaag aagagttcgt cctggagaat atcggcagag
     1621 agatagcata gccagcagaa ctcggtctag gtctcagaca ccaaacaaca ctgtcaccta
     1681 tgaaagtgaa cgaggaggtt ttaggcgtac attttcacgt tctgagcggg caggtgtgag
     1741 aacctatgtc agtaccatca gaattcccat tcgtagaatc ttaaatactg gtttaagtga
     1801 gactacatct gttgcaattc agaccatgtt aaggcagata atgacaggtt ttggtgagtt
     1861 aagctatttt atgtacagtg atagcgactc agagcctact ggctcagtct caaatcgaaa
     1921 tatggaaagg gcagagtcac ggagtggaag aggaggttct ggtggtggta gtagttctgg
     1981 ttccagttcg agttccagtt ccagttcgag ttccagttcc agttcaagtt ccagttccag
     2041 tcctagttcc agttccggtg gtgaaagttc agaaactagc tcagatttat ttgaaggcag
     2101 taatgaagga agctcatcat caggctcatc aggtgccagg cgagagggtc gacatagggc
     2161 cccagtcaca tttgatgaaa gtggctcttt gcccttcctt agcctggctc agtttttcct
     2221 cttaaatgag gatgatgatg accaacctag aggactcacc aaagaacaga ttgacaactt
     2281 ggcaatgaga agttttggtg aaaatgatgc attaaaaacc tgtagtgttt gcattacaga
     2341 atatacagaa ggcaacaaac ttcgtaaact accttgttcc catgagtacc atgtccactg
     2401 catcgatcgc tggttatctg agaattctac ctgtcctatt tgtcgcagag cagtcttagc
     2461 ttctggtaac agagaaagtg ttgtgtaccc ctacgacgtg cctgactacg ccggcggcgg
     2521 tggttcaggc ggtggcggtt ccggtggtgg gggatcagtc caactggtgg agtctggtgg
     2581 cgctttggtg cagccaggtg gctctctgcg tttgtcctgt gccgcttctg gcttcccagt
     2641 gaaccgctat tccatgcgct ggtatcgcca ggctccaggc aaagagcgtg agtgggtagc
     2701 cggtatgtcc agcgcgggtg atcgtagctc ctatgaagac tccgtgaagg gccgtttcac
     2761 catcagccgt gacgatgccc gtaacacggt gtatctgcaa atgaacagct tgaaacctga
     2821 agatacggcc gtgtattact gtaatgtgaa cgtgggcttc gagtattggg gccaaggcac
     2881 ccaggtcacc gtctccagct aagatccttg acttgcggcc gcaactccca cctgcaacat
     2941 gcgtgactga ctgaggccgc gactctagag tcgacctgca tctaggcgcc ggaattagat
     3001 ctctcgaggt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     3061 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     3121 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     3181 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     3241 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     3301 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     3361 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3421 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3481 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3541 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3601 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3661 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3721 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3781 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3841 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3901 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3961 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     4021 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4081 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4141 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4201 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcaacaaa
     4261 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgaccgagta
     4321 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4381 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4441 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4501 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4561 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4621 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     4681 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     4741 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     4801 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     4861 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat cttatcgata
     4921 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4981 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5041 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5101 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5161 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5221 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5281 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5341 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5401 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5461 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5521 gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5581 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     5821 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     5881 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     5941 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     6001 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     6061 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     6121 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     6181 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     6241 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     6301 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     6361 cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa
     6421 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6481 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6541 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6601 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6721 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6781 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6841 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6901 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6961 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7021 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7081 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7141 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7201 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7261 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7321 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7381 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7441 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7501 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7561 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7621 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7681 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7741 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7801 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7861 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7921 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7981 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8041 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8101 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8161 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8221 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8281 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8341 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8401 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8461 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8521 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8581 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8641 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8701 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8761 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8821 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8881 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8941 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     9001 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     9061 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9121 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9181 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9241 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     9301 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     9361 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     9421 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     9481 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     9541 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     9601 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     9661 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     9721 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     9781 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     9841 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     9901 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
     9961 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
    10021 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    10081 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    10141 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    10201 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    10261 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    10321 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    10381 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    10441 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    10501 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    10561 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    10621 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10681 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10741 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10801 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10861 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt
    10921 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10981 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    11041 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    11101 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    11161 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    11221 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    11281 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11341 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11401 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11461 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11521 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11581 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11641 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    11701 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    11761 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    11821 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    11881 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    11941 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    12001 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    12061 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    12121 gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg
    12181 atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    12241 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    12301 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    12361 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    12421 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    12481 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    12541 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    12601 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    12661 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    12721 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    12781 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    12841 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    12901 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    12961 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    13021 cgacggtcg
//
