LOCUS       V033917                14166 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033917
VERSION     V033917
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3622
                     /label="RNF111(NM_001330331)"
                     /note="RNF111(NM_001330331)"
                     /gene="RNF111"
     CDS             3623..3649
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3695..4036
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4162..4661
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4682..5389
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5390..5446
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5447..6046
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6057..6645
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6708..6941
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6966..6984)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7773..7877
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7878..8738
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8909..9497
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9743..10072
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11206..11271
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11401..11421
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11846..11980
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12149..12782
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12829..12954
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13446..13679
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13864..13908
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctctcagc agtgcagtga
      601 actccaagcc accatgtctc aatggactcc tgaatataac gagctctaca ccttaaaagt
      661 ggatatgaag agtgagattc cttctgatgc accaaagaca caggagagtc tgaaagggat
      721 ccttttgcat ccagagccca ttggggcagc caaaagtttt cctgcaggag ttgagatgat
      781 taatagtaaa gtggggaatg aattctctca cctgtgtgat gattctcaaa agcaagagaa
      841 ggaaatgaat ggtaaccagc aagaacaaga aaaaagtctc gttgtgagga aaaaacgcaa
      901 aagccagcag gctggccctt cgtatgtgca gaattgtgtt aaagaaaacc agggaatatt
      961 aggactgagg caacacctag ggacaccaag tgatgaagat aatgattcct cttttagtga
     1021 ttgtctttct tctccttcat ctagtctgca ttttggagat tctgatactg tgacttcaga
     1081 tgaggataaa gaagtctctg taagacattc ccagaccatt ttgaatgcta aaagtagaag
     1141 ccatagtgca cggtctcata agtggcctcg gactgagaca gaatctgtat cgggattgtt
     1201 aatgaaaaga ccctgtttac atggcagttc gttacggaga cttccatgca gaaagagatt
     1261 tgtaaaaaat aattcctcac agaggacaca gaaacaaaaa gagaggatat taatgcagag
     1321 gaagaaacga gaagtgttag ctcgaagaaa atatgccttg ctacctagtt ctagtagttc
     1381 cagtgagaat gacctcagca gtgaatcctc ttctagctca tcaactgaag gagaagaaga
     1441 tttgtttgtt tctgccagtg aaaaccacca aaacaatcca gctgttccct caggaagtat
     1501 tgatgaagat gttgtggtga tagaagcttc ctccactccc caggttactg ccaatgaaga
     1561 aattaatgtt acctcaactg acagtgaagt ggagattgta acagttggag aaagctatcg
     1621 gtctcgttca acccttggac actccagatc tcattggagc cagggttcca gttctcatgc
     1681 aagtcggcca caggagccac ggaaccgcag taggatttct actgttatac agcccttgag
     1741 gcagaatgca gcagaagttg tggaccttac cgttgatgaa gatgaaccta ctgtagtacc
     1801 aaccacttct gcaagaatgg aatcacaagc tactagcgct tccattaaca attcaaatcc
     1861 atctacctct gagcaggcct ctgatactgc ttcagctgtc accagtagcc aaccttccac
     1921 agtgtcagag acttcagcta ctcttacaag caatagtacc actggcactt ctataggaga
     1981 tgactcaagg agaactacat ctagtgctgt aacggaaact ggccctcctg caatgccaag
     2041 gttaccttcc tgctgtcccc agcactcacc atgtggaggg tcgtcacaga accaccatgc
     2101 attaggacat cctcatacaa gttgctttca gcagcatggt caccattttc aacatcatca
     2161 ccaccaccac catactcccc acccagctgt cccagtttct ccttccttta gtgatcctgc
     2221 ttgccctgtg gaaagacctc cacaagtaca agcaccttgt ggagcaaata gtagttctgg
     2281 taccagctat catgaacagc aggcattgcc agtggacctg agcaacagtg gtatcagaag
     2341 tcatggaagt ggcagttttc atggagcatc tgcatttgac ccctgctgcc ctgtttcttc
     2401 ctcccgagct gcaatctttg gccatcaggc cgctgctgct gccccaagtc aacctttatc
     2461 atcaatagat ggctatggat caagcatggt tgcgcagccc cagccccagc cccctccaca
     2521 gccctctctc tcatcatgtc gacattacat gccaccccct tatgcctctt tgacaaggcc
     2581 acttcatcat caagcttctg cctgcccgca ttctcatgga aacccccctc ctcagactca
     2641 gcctccgcct caagtggatt atgttattcc tcatcctgta catgctttcc attctcaaat
     2701 atcttctcat gcaacatctc atcctgtggc acccccacca ccaactcact tagccagtac
     2761 agctgcacca atccctcagc atcttcctcc tacacaccag ccaatttcgc accatattcc
     2821 agccacagca cctccagcac agagactgca tcctcatgaa gtgatgcaga ggatggaagt
     2881 tcaaaggagg aggatgatgc agcatccaac tggtcttttt gtgttctgtg tttccaggcg
     2941 ggcacatgaa cgccccccac cccatccaca taggatgcac ccaaactatg gtcatgggca
     3001 tcatattcat gtgcctcaga ctatgtcctc acatcctcga caggctccag agaggtctgc
     3061 ctgggaactg ggaattgaag ctggagtgac tgcagctact tatacacctg gtgcattgca
     3121 tcctcacttg gcccattatc acgcacctcc tcgacttcat cacttacaat taggagctct
     3181 tcctttaatg gttcctgata tggcaggcta tcctcacatc cgttacattt catcaggatt
     3241 ggatggaaca tcattcagag gtcctttcag gggcaatttt gaggaactga ttcatttgga
     3301 agaaagatta ggcaatgtca atcgtggagc atcccagggg acaattgaaa gatgtacata
     3361 tccacataaa tacaaaaagg taacaactga ttggttctca cagaggaaac tgcactgcaa
     3421 acaagatggg gaagaaggga ctgaggaaga cacagaggaa aaatgtacta tctgtttgtc
     3481 tattttagag gaaggtgaag atgtgagacg tcttccatgt atgcaccttt tccaccaagt
     3541 gtgtgttgac caatggttga ttaccaataa gaagtgcccc atatgcagag tggacattga
     3601 ggcccagctg ccaagtgaaa gttaccccta cgacgtgcct gactacgccg gcggcggtgg
     3661 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     3721 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     3781 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     3841 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     3901 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     3961 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     4021 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     4081 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     4141 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     4201 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     4261 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     4321 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     4381 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     4441 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     4501 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     4561 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4621 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4681 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4741 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4801 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4861 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4921 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4981 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     5041 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     5101 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     5161 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     5221 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     5281 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5341 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     5401 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     5461 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5521 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5581 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5641 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5701 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5761 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5821 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5881 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5941 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6001 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     6061 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6121 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6181 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6241 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6301 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6361 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6421 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6481 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6541 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6601 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     6661 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6721 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6781 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6841 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6901 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     6961 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     7021 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     7081 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     7141 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     7201 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     7261 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     7321 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     7381 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     7441 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     7501 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     7561 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7621 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     7681 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7741 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7801 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7861 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7921 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7981 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8041 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8101 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8161 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8221 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8281 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8341 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8401 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8461 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8521 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8581 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8641 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8701 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8761 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8881 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8941 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9001 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     9061 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9121 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9301 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9361 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9421 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9481 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9541 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9601 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9661 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9721 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9901 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    10021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
    10081 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
    10141 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    10201 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    10261 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    10321 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    10381 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    10441 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    10501 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    10561 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    10621 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    10681 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    10741 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    10801 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    10861 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    10921 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10981 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    11041 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    11101 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    11161 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    11221 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    11281 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    11341 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    11401 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    11461 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    11521 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    11581 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    11641 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    11701 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    11761 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    11821 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    11881 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11941 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    12001 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    12061 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    12121 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    12181 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    12241 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    12301 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    12361 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    12421 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    12481 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    12541 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    12601 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    12661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    12721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    12781 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    12841 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    12901 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12961 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    13021 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    13081 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    13141 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    13201 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    13261 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    13321 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    13381 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    13441 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    13501 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    13561 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    13621 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    13681 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    13741 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    13801 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    13861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    13921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    14041 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    14101 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    14161 cggtcg
//
