LOCUS       V034147                13059 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034147
VERSION     V034147
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2515
                     /label="RNF207(NM_207396)"
                     /note="RNF207(NM_207396)"
                     /gene="RNF207"
     CDS             2516..2542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2588..2929
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3055..3554
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3575..4282
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4283..4339
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4340..4939
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4950..5538
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5601..5834
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5859..5877)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6666..6770
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6771..7631
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7802..8390
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8636..8965
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10099..10164
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10294..10314
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10739..10873
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11042..11675
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11722..11847
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12339..12572
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12757..12801
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctacaccg gtcgcagtga
      601 actccaagcc accatgtcgg gagctatctt cgggcccctg gagggcccga gctccctgga
      661 tgccccgagc atccacccgc tggtgtgccc gctgtgccac gtgcagtacg agcgcccgtg
      721 tcttctggac tgtttccacg acttctgtgc cggctgcctg cgtggccgcg cgaccgacgg
      781 ccgcctcacc tgcccgctgt gccaacacca gacggtgctg aagggtccca gcgggctccc
      841 gccggtggac cggctgctgc agttcctggt ggacagctca ggggatggcg tggaggcggt
      901 gcgctgtgcc aactgtgacc tggagtgcag cgagcaggac gtggagacca cgtacttctg
      961 caacacgtgc ggacagcccc tatgcgcgcg ctgccgcgac gagacgcacc gagcacgcat
     1021 gttcgcgcgc cacgacatcg tggccctggg tcagcgaagc cgcgacgtgc cccagaagtg
     1081 cacgctgcac gcagagccct acctcttgtt ctccaccgac aagaagttgc tgttgtgcat
     1141 ccgctgcttc cgcgacatgc agaaggagag ccgggcacac tgcgtggacc tggaatcggc
     1201 ttacgtgcag ggctgcgagc ggctggagca ggcggtgctg gccgtgaagg ccctgcagac
     1261 ggccacgcgg gaggccatcg cgctgctgca ggccatggtg gaggaggtgc ggcacagcgc
     1321 cgccgaggag gaggacgcta tccacgccct cttcggcagc atgcaggaca ggctggcaga
     1381 gaggaaagcg ctgctgctgc aggctgtgca gagccaatac gaagagaagg acaaggcctt
     1441 caaggagcag ctctctcact tggccacctt gctgcccacc ctgcaggtcc acctggtcat
     1501 ctgctcctcc ttcctcagct tggccaacaa ggctgagttc ctggacctgg gctatgagct
     1561 gatggagagg ctgcagggca tcgtcacgcg gccgcaccac ctaaggccta ttcagagcag
     1621 caagattgcc agtgaccacc gagctgaatt cgcgcgctgt ctggagccac tgctgctgct
     1681 ggggccacgt cgggtggcag ctgctgcaag tggtgctaac acgctggcag ggggcttagg
     1741 ccccaaggcg ctgacggggc cccactgccc ctccccagta ggaaagatgt cggggtcacc
     1801 cgtccaaaag cccacgctgc accggtccat cagcaccaag gtgctgctgg cggagggcga
     1861 gaacacgccc ttcgcagagc actgccgcca ctatgaggac tcctaccggc acctgcaggc
     1921 agagatgcag agcctaaagg accaggtaca ggagctgcac cgagacctca ccaagcacca
     1981 ctcgctcatc aaggcggaga tcatgggaga cgtcctgcac aagtccctgc aactggacgt
     2041 gcagatcgcc tcggagcacg cctccttaga gggcatgagg gtcgtcttcc aggagatttg
     2101 ggaggaagcc tatcagcgag tggctaatga gcaggagatt tatgaagccc agctccatga
     2161 ccttctccag ctgaggcagg agaatgccta cctgaccacc atcaccaagc agatcacgcc
     2221 ctacgtccgc tccattgcca aggtgaagga gcggctggag cccaggtttc aggcacccgt
     2281 ggatgagcag tcagagagtc tacagaacac gcacgacgac agcaggaaca acgcggcctc
     2341 agccaggaat aatccaggaa gtgtcccgga aaagagagag aagacatcag agcctaaagg
     2401 aaacagctgg gctccgaacg gcctctcaga agagcctcta ctgaaaaata tggatcatca
     2461 cagatccaaa cagaaaaatg ggggcgatgt ccccacatgg agggaacacc cgacttaccc
     2521 ctacgacgtg cctgactacg ccggcggcgg tggttcaggc ggtggcggtt ccggtggtgg
     2581 gggatcagtc caactggtgg agtctggtgg cgctttggtg cagccaggtg gctctctgcg
     2641 tttgtcctgt gccgcttctg gcttcccagt gaaccgctat tccatgcgct ggtatcgcca
     2701 ggctccaggc aaagagcgtg agtgggtagc cggtatgtcc agcgcgggtg atcgtagctc
     2761 ctatgaagac tccgtgaagg gccgtttcac catcagccgt gacgatgccc gtaacacggt
     2821 gtatctgcaa atgaacagct tgaaacctga agatacggcc gtgtattact gtaatgtgaa
     2881 cgtgggcttc gagtattggg gccaaggcac ccaggtcacc gtctccagct aagatccttg
     2941 acttgcggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag
     3001 tcgacctgca tctaggcgcc ggaattagat ctctcgaggt taacgaattc taccgggtag
     3061 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     3121 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     3181 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     3241 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     3301 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     3361 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     3421 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     3481 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     3541 ctccgggcct ttcgacctgc agcccaagct taccatggtg agcaagggcg aggaggataa
     3601 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3661 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3721 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     3781 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     3841 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     3901 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     3961 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4021 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4081 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4141 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4201 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4261 cggcatggac gagctgtaca aggcaacaaa cttctctctg ctgaaacaag ccggagatgt
     4321 cgaagagaat cctggaccga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4381 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4441 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4501 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4561 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4621 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4681 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4741 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4801 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4861 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4921 ccgcaagccc ggtgcctgat cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4981 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5041 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5101 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5161 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5221 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5281 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5341 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5401 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5461 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5521 tttgggccgc ctccccgcat cgataccgtc gaccaaggca gctgtagatc ttagccactt
     5581 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5641 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5701 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5761 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5821 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     5881 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     5941 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     6001 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     6061 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     6121 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     6181 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     6241 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     6301 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     6361 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctagc tctgatgccg
     6421 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6481 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6541 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     6601 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6661 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6721 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6781 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6841 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6901 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6961 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7021 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7081 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7141 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7201 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7261 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7321 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7381 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7441 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7501 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7561 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7621 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7681 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7741 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7801 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7861 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7921 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7981 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8041 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8101 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8161 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8221 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8281 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8341 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8401 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8461 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8521 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8581 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     8641 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8701 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8761 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8821 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8881 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8941 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     9001 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     9061 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     9121 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     9181 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     9241 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     9301 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     9361 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     9421 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     9481 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     9541 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     9601 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     9661 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     9721 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     9781 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     9841 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     9901 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     9961 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    10021 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
    10081 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
    10141 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
    10201 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
    10261 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
    10321 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
    10381 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
    10441 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
    10501 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
    10561 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
    10621 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    10681 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    10741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10861 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    10921 ataccctatt accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct
    10981 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    11041 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
    11101 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
    11161 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    11221 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    11281 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    11341 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    11401 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    11461 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    11521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    11581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    11641 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    11701 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    11761 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    11821 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    11881 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    11941 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    12001 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    12061 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    12121 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    12181 caagcggccg gtgatcttca gacctggacg atatatatga gggacaattg gagaagtgaa
    12241 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    12301 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    12361 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    12421 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    12481 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    12541 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    12601 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    12661 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    12721 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    12781 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    12841 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    12901 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    12961 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    13021 gacagatcca ttcgattagt gaacggatct cgacggtcg
//
