LOCUS       V033574                13266 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033574
VERSION     V033574
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2722
                     /label="RNF214(NM_001077239)"
                     /note="RNF214(NM_001077239)"
                     /gene="RNF214"
     CDS             2723..2749
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2795..3136
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3262..3761
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3782..4489
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4490..4546
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4547..5146
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5157..5745
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5808..6041
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6066..6084)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6873..6977
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6978..7838
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8009..8597
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8843..9172
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10306..10371
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10501..10521
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10946..11080
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11249..11882
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11929..12054
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12546..12779
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12964..13008
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgacgaa actgcagtga
      601 actccaagcc accatggcag cgtctgaggt tgctggtgtt gtggccaatg cccccagtcc
      661 tccggaatct tctagtttat gtgcttccaa atcagacgaa ggtctcccag atggtctaag
      721 caccaaagac tctgcacaga agcagaagaa ctcgcctctg ttgagtgtaa gtagccaaac
      781 aataaccaag gagaataaca gaaatgtcca tttggagcac tcagagcaga atcctggttc
      841 atcagcaggt gacacctcag cagcgcacca ggtggtttta ggagaaaact tgatagccac
      901 agccctttgt ctttctggca gtgggtctca gtctgatttg aaggatgtgg ccagcacagc
      961 aggagaggag ggggacacaa gccttcggga gagcctccat ccagtcactc ggtctcttaa
     1021 ggcagggtgc catactaagc agcttgcctc caggaattgc tctgaagaga aatccccaca
     1081 aacctccatc ctaaaggaag gtaacaggga cacaagcttg gatttccgac ctgtagtgtc
     1141 tccagcaaat ggggttgaag gagtccgagt ggatcaggat gatgatcaag atagctcttc
     1201 cctgaagctt tctcagaaca ttgctgtaca gactgacttt aagacagctg attcagaggt
     1261 aaacacagat caagatattg aaaagaattt ggataaaatg atgacagaga gaaccctgtt
     1321 gaaagagcgt taccaggagg tcctggacaa acagaggcaa gtggagaatc agctccaagt
     1381 gcaattaaag cagcttcagc aaaggagaga agaggaaatg aagaatcacc aggagatatt
     1441 aaaggctatt caggatgtga caataaagcg ggaagaaaca aagaagaaga tagagaaaga
     1501 gaagaaggag tttttgcaga aggagcagga tctgaaagct gaaattgaga agctttgtga
     1561 gaagggcaga agagaggtgt gggaaatgga actggataga ctcaagaatc aggatggcga
     1621 aataaatagg aacattatgg aagagactga acgggcctgg aaggcagaga tcttatcact
     1681 agagagccgg aaagagttac tggtactgaa actagaagaa gcagaaaaag aggcagaatt
     1741 gcaccttact tacctcaagt caactccccc aacactggag acagttcgtt ccaaacagga
     1801 gtgggagacg agactgaatg gagttcggat aatgaaaaag aatgttcgtg accaatttaa
     1861 tagtcatatc cagttagtga ggaacggagc caagctgagc agccttcctc aaatccctac
     1921 tcccacttta cctccacccc catcagagac agacttcatg cttcaggtgt ttcaacccag
     1981 tccctctctg gctcctcgga tgcccttctc cattgggcag gtcacaatgc ccatggttat
     2041 gcccagtgca gatccccgct ccttgtcttt cccaatcctg aaccctgccc tttcccagcc
     2101 cagccagcct tcctcacccc ttcctggctc ccatggcaga aatagccctg gcttgggttc
     2161 ccttgtcagc ccccacggtc cacacatgcc ccctgccgcc tccatcccac ctcccccagg
     2221 cttgggcggt gttaaggctt ctgctgaaac tccccggccc caaccagtag acaaactgga
     2281 gaagatcctg gagaagctgc tgacccggtt cccacagtgc aataaggccc agatgaccaa
     2341 cattcttcag cagatcaaga cagcacgtac caccatggca ggcctgacca tggaggaact
     2401 tatccagttg gttgctgcac gactggcaga acatgagcgg gtggcagcaa gtactcagcc
     2461 acttggtcgc atccgggcct tgttccctgc tccactggcc caaatcagta ccccaatgtt
     2521 cttgccttct gcccaagttt catatcctgg aaggtcttca catgctccag ccacctgtaa
     2581 gctatgtcta atgtgccaga aactcgtcca gcccagtgag ctgcatccaa tggcgtgtac
     2641 ccatgtattg cacaaggagt gtatcaaatt ctgggcccag accaacacaa atgacacttg
     2701 tcccttttgt ccaactctta aataccccta cgacgtgcct gactacgccg gcggcggtgg
     2761 ttcaggcggt ggcggttccg gtggtggggg atcagtccaa ctggtggagt ctggtggcgc
     2821 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
     2881 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
     2941 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     3001 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     3061 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     3121 ggtcaccgtc tccagctaag atccttgact tgcggccgca actcccacct gcaacatgcg
     3181 tgactgactg aggccgcgac tctagagtcg acctgcatct aggcgccgga attagatctc
     3241 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3301 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3361 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3421 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3481 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3541 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     3601 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     3661 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     3721 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     3781 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     3841 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3901 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     3961 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4021 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4081 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4141 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4201 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4261 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4321 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     4381 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4441 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg caacaaactt
     4501 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatga ccgagtacaa
     4561 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4621 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4681 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4741 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4801 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     4861 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     4921 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     4981 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5041 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5101 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatctt atcgataatc
     5161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5521 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     5761 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5821 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5881 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5941 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6001 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     6061 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     6121 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     6181 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     6241 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     6301 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     6361 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     6421 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     6481 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     6541 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     6601 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     6661 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6721 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     6781 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6841 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6901 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6961 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7021 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7081 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7141 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7201 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7261 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7321 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7381 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7441 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7501 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7561 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7621 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7681 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7741 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7801 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8641 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8701 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8761 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8821 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9001 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9061 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9121 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     9181 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9241 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9301 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     9361 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     9421 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     9481 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     9541 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     9601 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     9661 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     9721 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     9781 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     9841 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     9901 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     9961 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    10021 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10081 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    10141 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    10201 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10261 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10321 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    10381 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    10441 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    10501 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    10561 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    10621 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    10681 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    10741 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    10801 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    10861 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    10921 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    10981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11041 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    11101 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    11161 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11221 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11281 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    11341 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    11401 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    11461 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    11521 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    11581 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    11641 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    11701 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    11761 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11821 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    11881 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    11941 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    12001 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12061 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    12121 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    12181 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12241 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12301 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    12361 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
    12421 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    12481 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    12541 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    12601 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    12661 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    12721 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    12781 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    12841 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    12901 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    12961 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    13021 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13081 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    13141 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    13201 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    13261 cggtcg
//
