LOCUS       V033297                12588 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033297
VERSION     V033297
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2044
                     /label="TRIM17(NM_001024940)"
                     /note="TRIM17(NM_001024940)"
                     /gene="TRIM17"
     CDS             2045..2071
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2117..2458
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2584..3083
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3104..3811
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3812..3868
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3869..4468
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4479..5067
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5130..5363
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5388..5406)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6195..6299
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6300..7160
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7331..7919
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8165..8494
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9628..9693
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9823..9843
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10268..10402
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10571..11204
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11251..11376
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11868..12101
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12286..12330
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgttcaa agagcagtga
      601 actccaagcc accatggagg ctgtggaact cgccagaaaa ctgcaggagg aagctacgtg
      661 ctccatctgt ctggattact tcacagaccc tgtgatgacc acctgtggcc acaacttctg
      721 ccgagcctgc atccagctga gctgggaaaa ggcgaggggc aagaagggga ggcggaagcg
      781 gaagggctcc ttcccctgcc ccgagtgcag agagatgtcc ccgcagagga acctgctgcc
      841 caaccggctg ctgaccaagg tggccgagat ggcgcagcag catcctggtc tgcagaagca
      901 agacctgtgc caggagcacc acgagcccct caagcttttc tgccagaagg accagagccc
      961 catctgtgtg gtgtgcaggg agtcccggga gcaccggctg cacagggtgc tgcccgccga
     1021 ggaggcagtg caggggtaca agttgaagct ggaggaggac atggagtacc ttcgggagca
     1081 gatcaccagg acagggaatc tgcaggccag ggaggagcag agcttagccg agtggcaggg
     1141 caaggtgaag gagcggagag aacgcattgt gctggagttt gagaagatga acctctacct
     1201 ggtggaagaa gagcagaggc tcctccaggc tctggagacg gaagaagagg agactgccag
     1261 caggctccgg gagagcgtgg cctgcctgga ccggcagggt cactctctgg agctgctgct
     1321 gctgcagctg gaggagcgga gcacacaggg gcccctccag atgctgcagg acatgaagga
     1381 acccctgagc aggaagaaca acgtgagtgt gcagtgccca gaggttgccc ccccaaccag
     1441 acccaggact gtgtgcagag ttcccggaca gattgaagtg ctaagaggct ttctagagga
     1501 tgtggtgcct gatgccacct ccgcgtaccc ctacctcctc ctgtatgaga gccgccagag
     1561 gcgctacctc ggctcttcgc cggagggcag tgggttctgc agcaaggacc gatttgtggc
     1621 ttacccctgt gctgtgggcc agacggcctt ctcctctggg aggcactact gggaggtggg
     1681 catgaacatc accggggacg cgttgtgggc cctgggtgtg tgcagggaca acgtgagccg
     1741 gaaagacagg gtccccaagt gccccgaaaa cggcttctgg gtggtgcagc tgtccaaggg
     1801 gaccaagtac ttatccacct tctctgccct aaccccggtc atgctgatgg agcctcccag
     1861 ccacatgggc atcttcctgg acttcgaagc cggggaagtg tccttctaca gtgtaagcga
     1921 tgggtcccac ctgcacacct actcccaggc caccttccca ggccccctgc agcctttctt
     1981 ctgcctgggg gctccgaagt ctggtcagat ggtcatctcc acagtgacca tgtgggtgaa
     2041 aggatacccc tacgacgtgc ctgactacgc cggcggcggt ggttcaggcg gtggcggttc
     2101 cggtggtggg ggatcagtcc aactggtgga gtctggtggc gctttggtgc agccaggtgg
     2161 ctctctgcgt ttgtcctgtg ccgcttctgg cttcccagtg aaccgctatt ccatgcgctg
     2221 gtatcgccag gctccaggca aagagcgtga gtgggtagcc ggtatgtcca gcgcgggtga
     2281 tcgtagctcc tatgaagact ccgtgaaggg ccgtttcacc atcagccgtg acgatgcccg
     2341 taacacggtg tatctgcaaa tgaacagctt gaaacctgaa gatacggccg tgtattactg
     2401 taatgtgaac gtgggcttcg agtattgggg ccaaggcacc caggtcaccg tctccagcta
     2461 agatccttga cttgcggccg caactcccac ctgcaacatg cgtgactgac tgaggccgcg
     2521 actctagagt cgacctgcat ctaggcgccg gaattagatc tctcgaggtt aacgaattct
     2581 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     2641 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     2701 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     2761 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     2821 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     2881 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     2941 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     3001 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     3061 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatggtga gcaagggcga
     3121 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3241 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3301 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3361 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3421 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3481 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3541 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3601 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3661 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3721 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3781 ctccaccggc ggcatggacg agctgtacaa ggcaacaaac ttctctctgc tgaaacaagc
     3841 cggagatgtc gaagagaatc ctggaccgat gaccgagtac aagcccacgg tgcgcctcgc
     3901 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     3961 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4021 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4081 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4141 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4201 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4261 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4321 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4381 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     4441 gtgcatgacc cgcaagcccg gtgcctgatc ttatcgataa tcaacctctg gattacaaaa
     4501 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4561 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4621 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4681 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4741 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4801 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4861 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     4921 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4981 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5041 ggatctccct ttgggccgcc tccccgcatc gataccgtcg accaaggcag ctgtagatct
     5101 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5161 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5221 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5281 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5341 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     5401 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     5461 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     5521 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     5581 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     5641 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     5701 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     5761 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     5821 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     5881 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctagct
     5941 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     6001 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6061 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     6121 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7441 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8101 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     8161 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8221 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8281 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8341 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8401 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8461 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     8521 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     8581 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     8641 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     8701 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     8761 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     8821 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     8881 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     8941 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     9001 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     9061 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     9121 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     9181 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     9241 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     9301 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     9361 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     9421 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     9481 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     9541 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     9601 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     9661 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     9721 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     9781 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     9841 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     9901 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     9961 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    10021 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    10081 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    10141 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    10201 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10261 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10321 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10381 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10441 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
    10501 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    10561 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
    10621 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
    10681 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
    10741 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
    10801 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
    10861 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
    10921 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
    10981 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
    11041 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    11101 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    11161 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    11221 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    11281 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    11341 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    11401 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    11461 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    11521 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    11581 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    11641 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    11701 accgcacagc aagcggccgg tgatcttcag acctggacga tatatatgag ggacaattgg
    11761 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    11821 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    11881 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    11941 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    12001 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    12061 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    12121 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    12181 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    12241 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    12301 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    12361 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    12421 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    12481 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    12541 agagacagag acagatccat tcgattagtg aacggatctc gacggtcg
//
