LOCUS       V030071                14307 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030071
VERSION     V030071
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3763
                     /label="TRIM24(NM_015905)"
                     /note="TRIM24(NM_015905)"
                     /gene="TRIM24"
     CDS             3764..3790
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3836..4177
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4303..4802
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4823..5530
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5531..5587
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5588..6187
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6198..6786
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6849..7082
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(7107..7125)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7914..8018
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8019..8879
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9050..9638
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9884..10213
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11347..11412
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11542..11562
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11987..12121
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12290..12923
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12970..13095
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13587..13820
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             14005..14049
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttgcccg taggcagtga
      601 actccaagcc accatggagg tggcggtgga gaaggcggtg gcggcggcgg cagcggcctc
      661 ggctgcggcc tccggggggc cctcggcggc gccgagcggg gagaacgagg ccgagagtcg
      721 gcagggcccg gactcggagc gcggcggcga ggcggcccgg ctcaacctgt tggacacttg
      781 cgccgtgtgc caccagaaca tccagagccg ggcgcccaag ctgctgccct gcctgcactc
      841 tttctgccag cgctgcctgc ccgcgcccca gcgctacctc atgctgcccg cgcccatgct
      901 gggctcggcc gagaccccgc cacccgtccc tgcccccggc tcgccggtca gcggctcgtc
      961 gccgttcgcc acccaagttg gagtcattcg ttgcccagtt tgcagccaag aatgtgcaga
     1021 gagacacatc atagataact tttttgtgaa ggacactact gaggttccca gcagtacagt
     1081 agaaaagtca aatcaggtat gtacaagctg tgaggacaac gcagaagcca atgggttttg
     1141 tgtagagtgt gttgaatggc tctgcaagac gtgtatcaga gctcatcaga gggtaaagtt
     1201 cacaaaagac cacactgtca gacagaaaga ggaagtatct ccagaggcag ttggtgtcac
     1261 cagccagcga ccagtgtttt gtccttttca taaaaaggag cagctgaagc tgtactgtga
     1321 gacatgtgac aaactgacat gtcgagactg tcagttgtta gaacataaag agcatagata
     1381 ccaatttata gaagaagctt ttcagaatca gaaagtgatc atagatacac taatcaccaa
     1441 actgatggaa aaaacaaaat acataaaatt cacaggaaat cagatccaaa acagaattat
     1501 tgaagtaaat caaaatcaaa agcaggtgga acaggatatt aaagttgcta tatttacact
     1561 gatggtagaa ataaataaaa aaggaaaagc tctactgcat cagttagaga gccttgcaaa
     1621 ggaccatcgc atgaaactta tgcaacaaca acaggaagtg gctggactct ctaaacaatt
     1681 ggagcatgtc atgcattttt ctaaatgggc agtttccagt ggcagcagta cagcattact
     1741 ttatagcaaa cgactgatta cataccggtt acggcacctc cttcgtgcaa ggtgtgatgc
     1801 atccccagtg accaacaaca ccatccaatt tcactgtgat cctagtttct gggctcaaaa
     1861 tatcatcaac ttaggttctt tagtaatcga ggataaagag agccagccac aaatgcctaa
     1921 gcagaatcct gtcgtggaac agaattcaca gccaccaagt ggtttatcat caaaccagtt
     1981 atccaagttc ccaacacaga tcagcctagc tcaattacgg ctccagcata tgcagcaaca
     2041 ggtaatggct cagaggcaac aggtgcaacg gaggccagca cctgtgggtt taccaaaccc
     2101 tagaatgcag gggcccatcc agcaaccttc catctctcat cagcaaccgc ctccacgttt
     2161 gataaacttt cagaatcaca gccccaaacc caatggacca gttcttcctc ctcatcctca
     2221 acaactgaga tatccaccaa accagaacat accacgacaa gcaataaagc caaaccccct
     2281 acagatggct ttcttggctc aacaagccat aaaacagtgg cagatcagca gtggacaggg
     2341 aaccccatca actaccaaca gcacatcctc tactccttcc agccccacga ttactagtgc
     2401 agcaggatat gatggaaagg cttttggttc acctatgatc gatttgagct caccagtggg
     2461 agggtcttat aatcttccct ctcttccgga tattgactgt tcaagtacta ttatgctgga
     2521 caatattgtg aggaaagata ctaatataga tcatggccag ccaagaccac cctcaaacag
     2581 aacggtccag tcaccaaatt catcagtgcc atctccaggc cttgcaggac ctgttactat
     2641 gactagtgta caccccccaa tacgttcacc tagtgcctcc agcgttggaa gccgaggaag
     2701 ctctggctct tccagcaaac cagcaggagc tgactctaca cacaaagtcc cagtggtcat
     2761 gctggagcca attcgaataa aacaagaaaa cagtggacca ccggaaaatt atgatttccc
     2821 tgttgttata gtgaagcaag aatcagatga agaatctagg cctcaaaatg ccaattatcc
     2881 aagaagcata ctcacctccc tgctcttaaa tagcagtcag agctctactt ctgaggagac
     2941 tgtgctaaga tcagatgccc ctgatagtac aggagatcaa cctggacttc accaggacaa
     3001 ttcctcaaat ggaaagtctg aatggttgga tccttcccag aagtcacctc ttcatgttgg
     3061 agagacaagg aaagaggatg accccaatga ggactggtgt gcagtttgtc aaaacggagg
     3121 ggaactcctc tgctgtgaaa agtgccccaa agtattccat ctttcttgtc atgtgcccac
     3181 attgacaaat tttccaagtg gagagtggat ttgcactttc tgccgagact tatctaaacc
     3241 agaagttgaa tatgattgtg atgctcccag tcacaactca gaaaaaaaga aaactgaagg
     3301 ccttgttaag ttaacaccta tagataaaag gaagtgtgag cgcctacttt tatttcttta
     3361 ctgccatgaa atgagcctgg cttttcaaga ccctgttcct ctaactgtgc ctgattatta
     3421 caaaataatt aaaaatccaa tggatttgtc aaccatcaag aaaagactac aagaagatta
     3481 ttccatgtac tcaaaacctg aagattttgt agctgatttt agattgatct ttcaaaactg
     3541 tgctgaattc aatgagcctg attcagaagt agccaatgct ggtataaaac ttgaaaatta
     3601 ttttgaagaa cttctaaaga acctctatcc agaaaaaagg tttcccaaac cagaattcag
     3661 gaatgaatca gaagataata aatttagtga tgattcagat gatgactttg tacagccccg
     3721 gaagaaacgc ctcaaaagca ttgaagaacg ccagttgctt aaatacccct acgacgtgcc
     3781 tgactacgcc ggcggcggtg gttcaggcgg tggcggttcc ggtggtgggg gatcagtcca
     3841 actggtggag tctggtggcg ctttggtgca gccaggtggc tctctgcgtt tgtcctgtgc
     3901 cgcttctggc ttcccagtga accgctattc catgcgctgg tatcgccagg ctccaggcaa
     3961 agagcgtgag tgggtagccg gtatgtccag cgcgggtgat cgtagctcct atgaagactc
     4021 cgtgaagggc cgtttcacca tcagccgtga cgatgcccgt aacacggtgt atctgcaaat
     4081 gaacagcttg aaacctgaag atacggccgt gtattactgt aatgtgaacg tgggcttcga
     4141 gtattggggc caaggcaccc aggtcaccgt ctccagctaa gatccttgac ttgcggccgc
     4201 aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcatc
     4261 taggcgccgg aattagatct ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     4321 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4381 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4441 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4501 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4561 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4621 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4681 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4741 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4801 cgacctgcag cccaagctta ccatggtgag caagggcgag gaggataaca tggccatcat
     4861 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     4921 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     4981 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     5041 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     5101 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5161 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     5221 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     5281 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     5341 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     5401 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     5461 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     5521 gctgtacaag gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     5581 tggaccgatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5641 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5701 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5761 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5821 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5881 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5941 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6001 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6061 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6121 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6181 tgcctgatct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6241 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6301 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6361 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6421 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6481 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6541 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6601 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6661 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6721 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6781 ccccgcatcg ataccgtcga ccaaggcagc tgtagatctt agccactttt taaaagaaaa
     6841 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6901 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6961 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7021 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7081 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     7141 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     7201 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     7261 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     7321 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     7381 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     7441 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     7501 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     7561 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     7621 ggagagcgtc gaatggtgca ctctcagtac aatctagctc tgatgccgca tagttaagcc
     7681 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7741 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7801 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7861 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9181 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9781 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9841 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     9901 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9961 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10021 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10081 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10141 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10201 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
    10261 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
    10321 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
    10381 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
    10441 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
    10501 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
    10561 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
    10621 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
    10681 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
    10741 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
    10801 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
    10861 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
    10921 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
    10981 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
    11041 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
    11101 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
    11161 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
    11221 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
    11281 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
    11341 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
    11401 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
    11461 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
    11521 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
    11581 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
    11641 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
    11701 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
    11761 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
    11821 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
    11881 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    11941 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    12001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    12061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    12121 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    12181 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12241 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    12301 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    12361 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    12421 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    12481 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    12541 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    12601 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    12661 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    12721 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    12781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    12841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    12901 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    12961 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    13021 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    13081 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    13141 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    13201 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    13261 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    13321 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    13381 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggt
    13441 gatcttcaga cctggacgat atatatgagg gacaattgga gaagtgaatt atataaatat
    13501 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    13561 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    13621 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    13681 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    13741 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    13801 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    13861 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    13921 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    13981 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    14041 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    14101 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    14161 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    14221 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    14281 cgattagtga acggatctcg acggtcg
//
