LOCUS       V033664                12495 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033664
VERSION     V033664
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1951
                     /label="TRIM43(NM_138800)"
                     /note="TRIM43(NM_138800)"
                     /gene="TRIM43"
     CDS             1952..1978
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2024..2365
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2491..2990
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3011..3718
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3719..3775
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3776..4375
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4386..4974
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5037..5270
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5295..5313)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6102..6206
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6207..7067
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7238..7826
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8072..8401
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9535..9600
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9730..9750
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10175..10309
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10478..11111
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11158..11283
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11775..12008
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12193..12237
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctatccat actgcagtga
      601 actccaagcc accatggact cagacttctc acatgccttc cagaaggaac tcacctgcgt
      661 catctgtttg aactacctgg tagaccctgt caccatctgc tgtgggcaca gcttctgtag
      721 gccctgtctc tgcctttcgt gggaggaagc ccaaagtcct gcaaactgcc ctgcatgcag
      781 ggaaccatca ccgaaaatgg acttcaaaac caatattctt ctgaagaatt tagtgaccat
      841 tgccagaaaa gccagtctct ggcaattcct gagctctgag aaacaaatat gtgggaccca
      901 taggcaaaca aagaagatgt tctgtgacat ggacaagagt ctcctctgct tgctgtgctc
      961 caactctcag gagcacgggg ctcacaaaca ccatcccatc gaagaggcag ctgaggaaca
     1021 ccgggagaaa ctcttaaagc aaatgaggat tttatggaaa aagattcaag aaaatcagag
     1081 aaatctatat gaggagggaa gaacagcctt cctctggagg ggcaatgtgg ttttacgggc
     1141 acagatgatc aggaatgagt ataggaagct gcatccggtt ctccataagg aagaaaaaca
     1201 acatttagag agactgaaca aggaatacca agagattttt cagcaactcc agagaagttg
     1261 ggtcaaaatg gatcaaaaga gtaaacactt gaaagaaatg tatcaggaac taatggaaat
     1321 gtgtcataaa ccagatgtgg agctgctcca ggatttggga gacatcgtgg caaggagtga
     1381 gtccgtgctg ctgcacatgc cccagcctgt gaatccagag ctcactgcag gacccatcac
     1441 tggactggtg tacaggctca accgcttccg agtggaaatt tccttccatt ttgaagtaac
     1501 caatcacaat atcaggctct ttgaggatgt gagaagttgg atgtttagac gtggaccttt
     1561 gaattctgac agatctgact attttgctgc atggggagcc agggtcttct cctttgggaa
     1621 acactactgg gagctggatg tggacaactc ttgtgactgg gctctgggag tctgtaacaa
     1681 ctcctggata aggaagaata gcacaatggt taactctgag gacatatttc ttcttttgtg
     1741 tctgaaggtg gataatcatt tcaatctctt gaccacctcc ccagtgtttc ctcactacat
     1801 agagaaacct ctgggccggg ttggtgtgtt tcttgatttt gaaagtggaa gtgtgagttt
     1861 tttgaatgtc accaagagtt ccctcatatg gagttaccca gctggctcct taacttttcc
     1921 tgtcaggcct ttcttttaca ctggccacag atacccctac gacgtgcctg actacgccgg
     1981 cggcggtggt tcaggcggtg gcggttccgg tggtggggga tcagtccaac tggtggagtc
     2041 tggtggcgct ttggtgcagc caggtggctc tctgcgtttg tcctgtgccg cttctggctt
     2101 cccagtgaac cgctattcca tgcgctggta tcgccaggct ccaggcaaag agcgtgagtg
     2161 ggtagccggt atgtccagcg cgggtgatcg tagctcctat gaagactccg tgaagggccg
     2221 tttcaccatc agccgtgacg atgcccgtaa cacggtgtat ctgcaaatga acagcttgaa
     2281 acctgaagat acggccgtgt attactgtaa tgtgaacgtg ggcttcgagt attggggcca
     2341 aggcacccag gtcaccgtct ccagctaaga tccttgactt gcggccgcaa ctcccacctg
     2401 caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcatcta ggcgccggaa
     2461 ttagatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     2521 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2581 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2641 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     2701 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     2761 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     2821 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     2881 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     2941 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     3001 caagcttacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3061 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3121 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3181 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     3241 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3301 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3361 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3421 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     3481 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     3541 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     3601 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3661 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     3721 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgac
     3781 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3841 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3901 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3961 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4021 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4081 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4141 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4201 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4261 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4321 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctta
     4381 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4441 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4501 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4561 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4621 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4681 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4741 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4801 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4861 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4921 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4981 accgtcgacc aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5041 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5101 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5161 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5221 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     5281 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     5341 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     5401 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     5461 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     5521 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     5581 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     5641 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     5701 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     5761 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     5821 atggtgcact ctcagtacaa tctagctctg atgccgcata gttaagccag ccccgacacc
     5881 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5941 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6001 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6061 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6421 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6481 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6721 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7381 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7861 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7921 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7981 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8041 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8101 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8161 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8221 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8281 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8341 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8401 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     8461 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     8521 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     8581 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     8641 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     8701 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     8761 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     8821 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     8881 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     8941 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     9001 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     9061 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     9121 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     9181 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     9241 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     9301 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     9361 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     9421 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     9481 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     9541 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     9601 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     9661 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     9721 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     9781 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     9841 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     9901 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     9961 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
    10021 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
    10081 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    10141 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    10201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10321 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10381 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10441 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10501 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10561 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10621 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10681 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10741 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10801 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10861 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    10921 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10981 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    11041 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    11101 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    11161 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    11221 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    11281 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    11341 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    11401 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    11461 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    11521 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11581 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggtga tcttcagacc
    11641 tggacgatat atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11701 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11761 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11821 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    11881 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11941 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    12001 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    12061 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    12121 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    12181 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    12241 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    12301 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    12361 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    12421 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    12481 ggatctcgac ggtcg
//
