LOCUS       V033185                13071 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033185
VERSION     V033185
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2527
                     /label="TRIM47(NM_033452)"
                     /note="TRIM47(NM_033452)"
                     /gene="TRIM47"
     CDS             2528..2554
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2600..2941
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3067..3566
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3587..4294
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4295..4351
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4352..4951
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4962..5550
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5613..5846
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5871..5889)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6678..6782
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6783..7643
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7814..8402
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8648..8977
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10111..10176
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10306..10326
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10751..10885
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11054..11687
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11734..11859
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12351..12584
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12769..12813
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgatcga acagcagtga
      601 actccaagcc accatggacg gcagtggacc cttcagctgc cccatctgcc tagagccact
      661 ccgggagccg gtgacgctgc cctgcggcca caacttctgt ctcgcctgcc tgggcgcgct
      721 ctggccgcat cgtggcgcga gtggagccgg cggacccgga ggcgcggccc gctgcccgct
      781 gtgccaggag cccttccccg acggccttca gctccgcaag aaccacacgc tgtccgagct
      841 gctgcagctc cgccagggct cgggccccgg gtccggcccc ggcccggccc ctgccctggc
      901 cccggagccc tcggcaccca gcgcgctgcc cagtgtcccg gagccgtcgg ccccctgcgc
      961 tcccgagccg tggcccgcgg gcgaagagcc agtgcgctgc gacgcgtgcc ccgagggcgc
     1021 ggccctgccc gccgcgctgt cctgcctctc ctgcctcgcc tccttttgcc ccgcgcacct
     1081 gggcccgcac gagcgcagcc ccgccctccg cggacaccgc ctggtgccgc cgctgcgccg
     1141 gctagaggag agcctgtgcc cgcgccacct acggccgctc gagcgctact gccgcgcgga
     1201 gcgcgtgtgt ctgtgcgagg cctgcgccgc acaggagcac cgcggccacg agctggtgcc
     1261 gctggagcag gagcgcgcgc ttcaggaggc tgagcagtcc aaagtcctga gcgccgtgga
     1321 ggaccgcatg gacgagctgg gtgctggcat tgcacagtcc aggcgcacag tggccctcat
     1381 caagagtgca gccgtagcag agcgggagag ggtgagccgg ctgtttgcag atgctgcggc
     1441 cgccctgcag ggcttccaga cccaggtgct gggcttcatc gaggaggggg aagctgccat
     1501 gctaggccgc tcccagggtg acctgcggcg acaggaggaa cagcgcagcc gcctgagccg
     1561 agcccgccag aatctcagcc aggtccctga agctgactca gtcagcttcc tgcaggagct
     1621 gctggcacta aggctggccc tggaggatgg gtgtggccct gggcctggac ccccgaggga
     1681 gctcagcttc accaaatcat cccaagctgt ccgtgcagtg agagacatgc tggccgtggc
     1741 ctgcgtcaac cagtgggagc agctgagggg gccgggtggc aacgaggatg ggccacagaa
     1801 gctggactcg gaagctgatg ctgagcccca agacctcgag agtacgaacc tcttggagag
     1861 tgaagctccc agggactatt tcctcaagtt tgcctatatt gtggatttgg acagcgacac
     1921 agcagacaag ttcctgcagc tgtttggaac caaaggtgtc aagagggtgc tgtgtcctat
     1981 caactacccc ttgtcgccca cccgcttcac ccattgtgag caggtgctgg gcgagggtgc
     2041 cctggaccga ggcacctact actgggaggt ggagattatc gagggctggg tcagcatggg
     2101 ggtcatggcc gaagacttct ccccacaaga gccctacgac cgcggccggc tgggccgcaa
     2161 cgcccactcc tgctgcctgc agtggaatgg acgcagcttc tccgtctggt ttcatgggct
     2221 ggaggctccc ctgccccacc ccttctcgcc cacggttggg gtctgcctgg aatacgctga
     2281 ccgtgccttg gccttctatg ctgtacggga cggcaagatg agcctcctgc ggaggctgaa
     2341 ggcctcccgg ccccgccggg gtggcatccc ggcctccccc attgacccct tccagagccg
     2401 cctggacagt cactttgcgg ggctcttcac ccacagactc aagcctgcct tcttcctgga
     2461 gagtgtggac gcccacttgc agatcgggcc cctcaagaag tcctgcatat ccgtgctgaa
     2521 gaggaggtac ccctacgacg tgcctgacta cgccggcggc ggtggttcag gcggtggcgg
     2581 ttccggtggt gggggatcag tccaactggt ggagtctggt ggcgctttgg tgcagccagg
     2641 tggctctctg cgtttgtcct gtgccgcttc tggcttccca gtgaaccgct attccatgcg
     2701 ctggtatcgc caggctccag gcaaagagcg tgagtgggta gccggtatgt ccagcgcggg
     2761 tgatcgtagc tcctatgaag actccgtgaa gggccgtttc accatcagcc gtgacgatgc
     2821 ccgtaacacg gtgtatctgc aaatgaacag cttgaaacct gaagatacgg ccgtgtatta
     2881 ctgtaatgtg aacgtgggct tcgagtattg gggccaaggc acccaggtca ccgtctccag
     2941 ctaagatcct tgacttgcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     3001 gcgactctag agtcgacctg catctaggcg ccggaattag atctctcgag gttaacgaat
     3061 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     3121 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     3181 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     3241 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     3301 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     3361 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     3421 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     3481 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     3541 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatgg tgagcaaggg
     3601 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     3661 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3721 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3781 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3841 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3901 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3961 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     4021 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     4081 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     4141 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     4201 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     4261 ccactccacc ggcggcatgg acgagctgta caaggcaaca aacttctctc tgctgaaaca
     4321 agccggagat gtcgaagaga atcctggacc gatgaccgag tacaagccca cggtgcgcct
     4381 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4441 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4501 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4561 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4621 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4681 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4741 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     4801 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     4861 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     4921 ctggtgcatg acccgcaagc ccggtgcctg atcttatcga taatcaacct ctggattaca
     4981 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5041 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5101 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5161 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5221 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5281 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5341 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5401 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5461 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5521 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgaccaagg cagctgtaga
     5581 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5641 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5701 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5761 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5821 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     5881 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     5941 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     6001 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     6061 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     6121 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     6181 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     6241 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     6301 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     6361 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatcta
     6421 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     6481 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     6541 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     6601 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6661 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6721 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6781 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6841 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6901 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6961 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7021 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7081 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7141 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7201 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7261 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7321 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7381 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7441 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7501 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7561 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7621 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7681 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7741 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7801 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7861 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7921 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7981 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8041 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8101 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8161 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8221 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8281 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8341 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8401 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8461 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8521 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8581 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8641 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8701 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8821 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8881 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8941 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     9001 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     9061 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     9121 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     9181 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     9241 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     9301 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     9361 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     9421 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     9481 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     9541 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     9601 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     9661 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     9721 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     9781 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     9841 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     9901 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     9961 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
    10021 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
    10081 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
    10141 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
    10201 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
    10261 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
    10321 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
    10381 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
    10441 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
    10501 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
    10561 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
    10621 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
    10681 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    10741 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10861 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    10921 acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa
    10981 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11041 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
    11101 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
    11161 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
    11221 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
    11281 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
    11341 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
    11401 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
    11461 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
    11521 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    11581 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    11641 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    11701 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    11761 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    11821 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    11881 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    11941 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    12001 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    12061 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    12121 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    12181 accaccgcac agcaagcggc cggtgatctt cagacctgga cgatatatat gagggacaat
    12241 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    12301 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    12361 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    12421 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    12481 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    12541 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    12601 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    12661 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    12721 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    12781 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    12841 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    12901 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    12961 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    13021 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggtc g
//
