LOCUS       V033348                12615 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033348
VERSION     V033348
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2071
                     /label="TRIM58(NM_015431)"
                     /note="TRIM58(NM_015431)"
                     /gene="TRIM58"
     CDS             2072..2098
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2144..2485
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2611..3110
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3131..3838
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3839..3895
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3896..4495
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4506..5094
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5157..5390
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5415..5433)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6222..6326
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6327..7187
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7358..7946
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8192..8521
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9655..9720
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9850..9870
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10295..10429
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10598..11231
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11278..11403
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11895..12128
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12313..12357
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgtgact ctggcagtga
      601 actccaagcc accatggcct gggcgccgcc cggggagcgg ctgcgcgagg atgcgcggtg
      661 cccggtgtgc ctggatttcc tgcaggagcc ggtcagcgtg gactgcggcc acagcttctg
      721 cctcaggtgc atctccgagt tctgcgagaa gtcggacggc gcgcagggcg gcgtctacgc
      781 ctgtccgcag tgccggggcc ccttccggcc ctcgggcttt cgccccaacc ggcagctggc
      841 gggcctggtg gagagcgtgc ggcggctggg gttgggcgcg gggcccgggg cgcggcgatg
      901 cgcgcggcac ggcgaggacc tgagccgctt ctgcgaggag gacgaggcgg cgctgtgctg
      961 ggtgtgcgac gccggccccg agcacaggac gcaccgcacg gcgccgctgc aggaggccgc
     1021 cggcagctac caggtaaagc tccagatggc tctggaactt atgaggaaag agttggagga
     1081 cgccttgact caggaggcca acgtggggaa aaagactgtc atttggaagg agaaagtgga
     1141 aatgcagagg cagcgcttca gattggagtt tgagaagcat cgtggctttc tggcccagga
     1201 ggagcaacgg cagctgaggc ggctggaggc ggaggagcga gcgacgctgc agagactgcg
     1261 ggagagcaag agccggctgg tccagcagag caaggccctg aaggagctgg cggatgagct
     1321 gcaggagagg tgccagcgcc cggccctggg tctgctggag ggtgtgagag gagtcctgag
     1381 cagaagtaag gctgtcacaa ggctggaagc agagaacatc cccatggaac tgaagacagc
     1441 atgctgcatc cctgggagga gggagctctt aaggaagttc caagtggatg taaagctgga
     1501 tcccgccacg gcgcacccga gtctgctctt gaccgccgac ctgcgcagtg tgcaggatgg
     1561 agaaccatgg agggatgtcc ccaacaaccc tgagcgattt gacacatggc cctgcatcct
     1621 gggtttgcag agcttctcat cagggaggca ttactgggag gttctggtgg gagaaggagc
     1681 agagtggggt ttaggggtct gtcaagacac actgccaaga aagggggaaa ccacgccatc
     1741 tcctgagaat ggggtctggg ccctgtggct gctgaaaggg aatgagtaca tggtccttgc
     1801 ctccccatca gtgcctcttc tccaactgga aagtcctcgc tgcattggga ttttcttgga
     1861 ctatgaagcc ggtgaaattt cattctacaa tgtcacagat ggatcttata tctacacatt
     1921 caaccaactc ttctctggtc ttcttcggcc ttactttttc atctgtgatg caactcctct
     1981 tatcttgcca cccacaacaa tagcagggtc aggaaattgg gcatccaggg atcatttaga
     2041 tcctgcttct gatgtaagag atgatcatct ctacccctac gacgtgcctg actacgccgg
     2101 cggcggtggt tcaggcggtg gcggttccgg tggtggggga tcagtccaac tggtggagtc
     2161 tggtggcgct ttggtgcagc caggtggctc tctgcgtttg tcctgtgccg cttctggctt
     2221 cccagtgaac cgctattcca tgcgctggta tcgccaggct ccaggcaaag agcgtgagtg
     2281 ggtagccggt atgtccagcg cgggtgatcg tagctcctat gaagactccg tgaagggccg
     2341 tttcaccatc agccgtgacg atgcccgtaa cacggtgtat ctgcaaatga acagcttgaa
     2401 acctgaagat acggccgtgt attactgtaa tgtgaacgtg ggcttcgagt attggggcca
     2461 aggcacccag gtcaccgtct ccagctaaga tccttgactt gcggccgcaa ctcccacctg
     2521 caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcatcta ggcgccggaa
     2581 ttagatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     2641 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2701 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2761 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     2821 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     2881 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     2941 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     3001 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     3061 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     3121 caagcttacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3181 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3241 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3301 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     3361 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3421 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3481 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3541 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     3601 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     3661 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     3721 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3781 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     3841 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgac
     3901 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3961 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4021 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4081 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4141 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4201 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4261 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4321 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4381 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4441 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctta
     4501 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4561 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4621 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4681 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4741 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4801 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4861 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4921 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4981 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5041 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5101 accgtcgacc aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5161 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5221 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5281 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5341 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     5401 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     5461 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     5521 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     5581 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     5641 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     5701 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     5761 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     5821 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     5881 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     5941 atggtgcact ctcagtacaa tctagctctg atgccgcata gttaagccag ccccgacacc
     6001 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     6061 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6121 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6181 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7501 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8101 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8221 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8341 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8401 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8461 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8521 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     8581 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     8641 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     8701 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     8761 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     8821 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     8881 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     8941 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     9001 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     9061 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     9121 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     9181 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     9241 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     9301 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     9361 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     9421 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     9481 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     9541 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     9601 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     9661 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     9721 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     9781 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     9841 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     9901 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     9961 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
    10021 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
    10081 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
    10141 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
    10201 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    10261 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    10321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10441 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10501 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10561 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10621 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10681 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10741 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10801 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10861 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10921 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10981 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    11041 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    11101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    11161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    11221 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    11281 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    11341 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    11401 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    11461 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    11521 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    11581 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    11641 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11701 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggtga tcttcagacc
    11761 tggacgatat atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11821 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11881 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11941 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    12001 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    12061 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    12121 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    12181 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    12241 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    12301 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    12361 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    12421 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    12481 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    12541 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    12601 ggatctcgac ggtcg
//
