LOCUS       V033632                12504 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033632
VERSION     V033632
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1960
                     /label="TRIM64B(NM_001164397)"
                     /note="TRIM64B(NM_001164397)"
                     /gene="TRIM64B"
     CDS             1961..1987
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2033..2374
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2500..2999
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3020..3727
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3728..3784
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3785..4384
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4395..4983
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5046..5279
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5304..5322)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6111..6215
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6216..7076
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7247..7835
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8081..8410
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9544..9609
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9739..9759
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10184..10318
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10487..11120
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11167..11292
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11784..12017
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12202..12246
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgtagtt atcgcagtga
      601 actccaagcc accatggatt cagacgacct gcaagtcttc cagaatgagc tcatttgctg
      661 catttgcgtg aactacttca tagatccggt caccattgac tgtgggcaca gcttttgcag
      721 gccctgcctc tgcctctgct cagaagaagg cagagcacca atgcgctgcc cttcgtgcag
      781 aaaaacctca gagaagccca acttcaacac caatctggta ctcaaaaagc tgtcttccct
      841 agccagacag accagacctc agaacatcaa cagctcagac aatatctgtg tgctccatga
      901 ggagactaag gagctcttct gtgaggctga caagagattg ctctgtgggc cctgctctga
      961 gtcaccagag cacatggctc acagccacag cccaatagga tgggctgctg aggaatgcag
     1021 ggagaaactt ataaaggaaa tggactattt atgggaaatc aatcaagaga caagaaacaa
     1081 tctaaatcag gaaactagca catttcattc gttaaaggac tatgtgtcag taaggaagag
     1141 gataatcact attcaatatc aaaagatgcc tatatttctc gatgaggagg agcaacggca
     1201 tctgcaggca ctggaaagag aagcagaaga gcttttccaa caactacaag acagtcaagt
     1261 gagaatgacc caacatttag aaaggatgaa agacatgtac agagagctgt gggagacatg
     1321 ccacatgcct gacgtggtgc tgctccagga tgtgagaaat gtatcagcaa ggactgattt
     1381 ggcacagatg caaaagcccc agccagtgaa cccagagctc acttcatggt gcataactgg
     1441 agtcctagac atgctcaaca acttcagagt ggatagtgct ctgagcacgg aaatgattcc
     1501 ttgctatata agcctttctg aggatgtgag atatgtgata tttggagatg accatctcag
     1561 tgctcccacg gatccccagg gagtggacag ctttgctgtg tggggagcgc aagcattcac
     1621 ctccggcaag cattactggg aggtggatgt gaccctctcc tccaactgga ttctgggagt
     1681 ctgtcgagat tccaggactg cagatgccaa tttcgttatt gattctgatg aaagattttt
     1741 tttaatttcc tcaaagagga gcaatcacta tagtctctcc accaactctc cacctttaat
     1801 tcagtatgtg caaaggcctc tgggtcgagt tggggtgttt ctggattatg ataatggatc
     1861 tgtgagtttt tttgatgttt ctaaaggttc tcttatctat ggttttcctc cttcctcctt
     1921 ctcttcccct ctgaggcctt tcttttgctt tggttgtaca tacccctacg acgtgcctga
     1981 ctacgccggc ggcggtggtt caggcggtgg cggttccggt ggtgggggat cagtccaact
     2041 ggtggagtct ggtggcgctt tggtgcagcc aggtggctct ctgcgtttgt cctgtgccgc
     2101 ttctggcttc ccagtgaacc gctattccat gcgctggtat cgccaggctc caggcaaaga
     2161 gcgtgagtgg gtagccggta tgtccagcgc gggtgatcgt agctcctatg aagactccgt
     2221 gaagggccgt ttcaccatca gccgtgacga tgcccgtaac acggtgtatc tgcaaatgaa
     2281 cagcttgaaa cctgaagata cggccgtgta ttactgtaat gtgaacgtgg gcttcgagta
     2341 ttggggccaa ggcacccagg tcaccgtctc cagctaagat ccttgacttg cggccgcaac
     2401 tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcatctag
     2461 gcgccggaat tagatctctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc
     2521 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     2581 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     2641 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     2701 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     2761 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     2821 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     2881 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     2941 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3001 cctgcagccc aagcttacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     3061 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     3121 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     3181 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     3241 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     3301 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     3361 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     3421 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3481 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     3541 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     3601 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     3661 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     3721 gtacaaggca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     3781 accgatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     3841 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     3901 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     3961 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4021 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4081 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4141 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4201 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4261 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4321 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4381 ctgatcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4441 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4501 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4561 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4621 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4681 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4741 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4801 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4861 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4921 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4981 cgcatcgata ccgtcgacca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5041 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5101 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5161 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5221 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5281 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     5341 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     5401 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     5461 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     5521 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     5581 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     5641 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     5701 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     5761 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5821 gagcgtcgaa tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc
     5881 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5941 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6001 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6061 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6121 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6181 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6241 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6301 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6361 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6421 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6481 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6541 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6601 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6661 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6721 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6781 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6841 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6901 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6961 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7021 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7081 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7141 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7201 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7261 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7321 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7381 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7441 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7501 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7561 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7621 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7681 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7741 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7801 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7861 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7921 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7981 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8041 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     8101 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8161 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8221 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8281 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8341 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8401 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     8461 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     8521 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     8581 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     8641 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     8701 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     8761 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     8821 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     8881 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     8941 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     9001 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     9061 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     9121 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     9181 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     9241 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     9301 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     9361 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     9421 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     9481 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     9541 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     9601 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     9661 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     9721 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     9781 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     9841 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     9901 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     9961 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    10021 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    10081 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    10141 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    10201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10261 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10321 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10381 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10441 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    10501 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    10561 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    10621 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    10681 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    10741 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10801 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    10861 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10921 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10981 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    11041 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    11101 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    11161 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    11221 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    11281 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    11341 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    11401 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11461 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    11521 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    11581 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat
    11641 cttcagacct ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa
    11701 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    11761 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    11821 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    11881 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    11941 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    12001 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    12061 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    12121 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    12181 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    12241 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    12301 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    12361 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    12421 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
    12481 ttagtgaacg gatctcgacg gtcg
//
