LOCUS       V033910                12507 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033910
VERSION     V033910
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1963
                     /label="TRIM77(NM_001146162)"
                     /note="TRIM77(NM_001146162)"
                     /gene="TRIM77"
     CDS             1964..1990
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2036..2377
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2503..3002
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3023..3730
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3731..3787
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3788..4387
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4398..4986
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5049..5282
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5307..5325)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6114..6218
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6219..7079
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7250..7838
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8084..8413
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9547..9612
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9742..9762
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10187..10321
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10490..11123
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11170..11295
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11787..12020
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12205..12249
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctggaagg tcagcagtga
      601 actccaagcc accatggctt ctgctatcac gcagtgttct accagtgagc tcacctgctc
      661 gatctgcaca gactatttga cagaccctgt caccatttgt tgtgggcaca gattttgtag
      721 tccctgtctc tgcctcttgt gggaagatac actaactcct aattgctgcc ctgtgtgcag
      781 ggaaatatca cagcaaatgt acttcaaacg cattattttt gctgagaaac aagttattcc
      841 tacaagagaa tcagtcccct gccagttatc aagctctgcc atgctgatct gtaggagaca
      901 ccaggaaatc aagaacctca tctgtgaaac tgataggagc ctgctgtgtt ttctatgctc
      961 tcaatcccca aggcatgcta ctcacaaaca ctatatgaca agggaggctg atgaatacta
     1021 taggaagaaa ctcctgattc aaatgaagtc catctggaaa aaaaaacaga aaaatcaaag
     1081 aaatctaaac agagagacca acataatcgg aacatgggaa gtttttataa atttgcggag
     1141 catgatgatc agtgctgaat atcctaaggt gtgtcaatac ctccgtgaag aagagcaaaa
     1201 gcacgtagag agcctggcaa gagaaggcag gataattttt cagcaactca agagaagtca
     1261 aactagaatg gctaagatgg gtatactcct gagagaaatg tatgagaaac tgaaggaaat
     1321 gagctgtaaa gcagatgtga acctgcctca ggatttggga gacgtaatga aaaggaatga
     1381 gtttctgagg ctggccatgc ctcagcctgt gaacccacag ctcagtgcat ggaccatcac
     1441 tggggtgtca gaaaggctta acttcttcag agtgtatatc accttggatc gtaagatatg
     1501 cagtaatcac aagcttctgt tcgaagacct aaggcatttg cagtgcagcc ttgatgatac
     1561 agacatgtcc tgtaatccaa caagtacaca gtatacttct tcatggggag ctcagatcct
     1621 cagctctggc aaacattact gggaggtgga tgtgaaagac tcttgtaatt gggttatagg
     1681 actttgcaga gaagcctgga caaagaggaa tgacatgcga cttgactctg agggtatctt
     1741 tctactcctg tgtctcaaag tggatgacca tttcagtctc ttctctacct ccccactgtt
     1801 acctcactat attccaaggc cccaaggctg gctaggggtg ttcctggatt atgaatgtgg
     1861 gatagtgagc tttgttaatg ttgcccaaag ttccctcatt tgtagtttcc tctcacgcat
     1921 cttctatttt cctctcagac ctttcatttg ccacggatct aaatacccct acgacgtgcc
     1981 tgactacgcc ggcggcggtg gttcaggcgg tggcggttcc ggtggtgggg gatcagtcca
     2041 actggtggag tctggtggcg ctttggtgca gccaggtggc tctctgcgtt tgtcctgtgc
     2101 cgcttctggc ttcccagtga accgctattc catgcgctgg tatcgccagg ctccaggcaa
     2161 agagcgtgag tgggtagccg gtatgtccag cgcgggtgat cgtagctcct atgaagactc
     2221 cgtgaagggc cgtttcacca tcagccgtga cgatgcccgt aacacggtgt atctgcaaat
     2281 gaacagcttg aaacctgaag atacggccgt gtattactgt aatgtgaacg tgggcttcga
     2341 gtattggggc caaggcaccc aggtcaccgt ctccagctaa gatccttgac ttgcggccgc
     2401 aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcatc
     2461 taggcgccgg aattagatct ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     2521 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     2581 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     2641 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     2701 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     2761 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     2821 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     2881 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     2941 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     3001 cgacctgcag cccaagctta ccatggtgag caagggcgag gaggataaca tggccatcat
     3061 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     3121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     3241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     3301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     3361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     3421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     3481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     3541 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     3601 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     3661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     3721 gctgtacaag gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     3781 tggaccgatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     3841 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     3901 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     3961 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     4021 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     4081 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     4141 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     4201 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     4261 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     4321 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     4381 tgcctgatct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4441 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4501 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4561 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4621 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4681 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4741 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4801 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4861 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4921 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4981 ccccgcatcg ataccgtcga ccaaggcagc tgtagatctt agccactttt taaaagaaaa
     5041 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     5101 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5161 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5221 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5281 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     5341 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     5401 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     5461 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     5521 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     5581 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     5641 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     5701 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     5761 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     5821 ggagagcgtc gaatggtgca ctctcagtac aatctagctc tgatgccgca tagttaagcc
     5881 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5941 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6001 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     6061 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6121 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6181 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6241 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6301 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6361 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6421 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6481 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6541 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6601 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6661 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6721 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6781 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6841 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6901 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6961 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7021 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7081 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7141 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7381 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7441 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7501 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7561 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7621 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7681 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7741 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7801 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7861 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7921 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7981 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8041 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     8101 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8161 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8221 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8281 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8341 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8401 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     8461 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     8521 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     8581 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     8641 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     8701 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     8761 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     8821 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     8881 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     8941 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     9001 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     9061 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     9121 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     9181 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     9241 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     9301 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     9361 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     9421 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     9481 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     9541 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     9601 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     9661 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     9721 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     9781 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     9841 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     9901 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     9961 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
    10021 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
    10081 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    10141 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    10201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    10321 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    10381 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    10441 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    10501 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    10561 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    10621 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    10681 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    10741 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    10801 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    10861 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    10921 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10981 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    11041 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    11101 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    11161 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    11221 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    11281 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    11341 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    11401 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    11461 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    11521 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    11581 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggt
    11641 gatcttcaga cctggacgat atatatgagg gacaattgga gaagtgaatt atataaatat
    11701 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    11761 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    11821 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    11881 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    11941 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    12001 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    12061 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    12121 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    12181 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    12241 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    12301 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    12361 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    12421 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    12481 cgattagtga acggatctcg acggtcg
//
