LOCUS       V033465                12543 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033465
VERSION     V033465
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1999
                     /label="TRIML2(NM_001303419)"
                     /note="TRIML2(NM_001303419)"
                     /gene="TRIML2"
     CDS             2000..2026
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2072..2413
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2539..3038
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3059..3766
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3767..3823
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3824..4423
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4434..5022
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5085..5318
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5343..5361)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6150..6254
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6255..7115
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7286..7874
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8120..8449
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9583..9648
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9778..9798
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10223..10357
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10526..11159
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11206..11331
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11823..12056
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12241..12285
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctacttta cgagcagtga
      601 actccaagcc accatgtcca aaaggctcag ccctcagtta cagcacaaca tcacagaaga
      661 tgcctattgt gaaacacacc tggaaccaac acggctgttc tgtgatgttg accaaatcac
      721 actctgcagc aaatgcttcc agtcccagga gcacaaacat cacatggtgt gtgggataca
      781 agaggctgct gagaattaca ggaagttatt ccaggaaata ttgaacacat cgagggagaa
      841 acttgaagca gctaaaagca tattgactga tgagcaagaa agaatggcga tgattcagga
      901 agaggaacaa aattttaaaa agatgattga gtctgagtat agtatgagac tccggttgtt
      961 gaatgaagag tgtgagcaga atctccagag acagcaggaa tgcatatctg acttgaacct
     1021 gagagaaacc cttctgaatc aagcgatcaa gcttgccacc gagctagagg agatgttcca
     1081 ggaaatgcta cagagactgg gccgtgtggg gagagagaac atggagaaac tgaaggagag
     1141 tgaagccagg gcttctgaac aggtccgcag cctcctaaag ctcatcgtgg agcttgagaa
     1201 aaagtgtggg gaaggcacct tggcattgct caagtgcctt gatcttgaaa ttcggcctcc
     1261 agaactgcga gaaatgaatt tctgctgtgc agaagctact cagtctatga atgcaaaata
     1321 ctctttagaa aggagcaagt cactgctgct tgagcatctg gagcccgctc atatcacaga
     1381 cctgagttta tgccacataa gaggactcag cagcatgttc agagtactcc agagacattt
     1441 aacattggat cctgaaacag ctcatccctg cctggcacta tctgaggacc tgagaactat
     1501 gagattgaga catgggcagc aggatggggc tggcaaccca gaaagattgg atttcagtgc
     1561 catggtgctg gctgcggaga gcttcacctc agggaggcac tactgggagg tggacgtgga
     1621 aaaggcaacc aggtggcaag tgggcatata ccacggctct gcagacgcga agggcagcac
     1681 ggccagagct tccggagaga aagtcttgct cacggggtcg gtgatgggga ccgagtggac
     1741 tctctgggtc ttcccccctc tgaaaaggct cttcctggaa aagaagttgg acacagttgg
     1801 cgttttcctt gactgcgaac acgggcagat atcattctac aatgtgaccg agatgtccct
     1861 catttacaat ttctcccatt gcgccttcca aggagctctc aggcctgtgt tttccctctg
     1921 tatcccaaat ggagacacaa gtccagactc cctcaccatc ttacaacatg gtccttcttg
     1981 tgatgctact gttagccctt acccctacga cgtgcctgac tacgccggcg gcggtggttc
     2041 aggcggtggc ggttccggtg gtgggggatc agtccaactg gtggagtctg gtggcgcttt
     2101 ggtgcagcca ggtggctctc tgcgtttgtc ctgtgccgct tctggcttcc cagtgaaccg
     2161 ctattccatg cgctggtatc gccaggctcc aggcaaagag cgtgagtggg tagccggtat
     2221 gtccagcgcg ggtgatcgta gctcctatga agactccgtg aagggccgtt tcaccatcag
     2281 ccgtgacgat gcccgtaaca cggtgtatct gcaaatgaac agcttgaaac ctgaagatac
     2341 ggccgtgtat tactgtaatg tgaacgtggg cttcgagtat tggggccaag gcacccaggt
     2401 caccgtctcc agctaagatc cttgacttgc ggccgcaact cccacctgca acatgcgtga
     2461 ctgactgagg ccgcgactct agagtcgacc tgcatctagg cgccggaatt agatctctcg
     2521 aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     2581 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     2641 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     2701 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     2761 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     2821 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     2881 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     2941 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3001 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     3061 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3121 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3181 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3241 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3301 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3361 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     3421 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     3481 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     3541 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3601 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3661 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     3721 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcaa caaacttctc
     3781 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatgaccg agtacaagcc
     3841 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3901 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     3961 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4021 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4081 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4141 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4201 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4261 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4321 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4381 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatcttatc gataatcaac
     4441 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4501 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4561 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4621 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4681 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4741 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4801 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4861 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4921 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4981 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgaccaa
     5041 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5101 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5161 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5221 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5281 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     5341 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     5401 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     5461 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     5521 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     5581 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     5641 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     5701 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     5761 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     5821 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     5881 cagtacaatc tagctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5941 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6001 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6061 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6121 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6181 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6241 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6481 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6781 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7261 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7321 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7381 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7441 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7501 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7561 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7621 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7681 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7741 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7801 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7861 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7921 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7981 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8041 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8101 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8221 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8281 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8341 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8401 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     8461 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     8521 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     8581 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     8641 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     8701 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     8761 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     8821 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     8881 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     8941 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     9001 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     9061 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     9121 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     9181 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     9241 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     9301 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     9361 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     9421 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
     9481 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
     9541 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     9601 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     9661 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     9721 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     9781 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     9841 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     9901 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     9961 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    10021 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    10081 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    10141 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    10201 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    10261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10321 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10381 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    10441 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10501 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    10561 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    10621 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    10681 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    10741 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    10801 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    10861 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    10921 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    10981 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    11041 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    11101 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    11161 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    11221 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    11281 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11341 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11401 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11461 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11521 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11581 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11641 aacaaaagta agaccaccgc acagcaagcg gccggtgatc ttcagacctg gacgatatat
    11701 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    11761 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    11821 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    11881 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    11941 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    12001 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    12061 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    12121 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    12181 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    12241 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    12301 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    12361 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    12421 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    12481 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    12541 tcg
//
