LOCUS       V033362                12699 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033362
VERSION     V033362
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2155
                     /label="ZBTB2(NM_020861)"
                     /note="ZBTB2(NM_020861)"
                     /gene="ZBTB2"
     CDS             2156..2182
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2228..2569
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2695..3194
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3215..3922
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3923..3979
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3980..4579
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4590..5178
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5241..5474
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5499..5517)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6306..6410
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6411..7271
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7442..8030
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8276..8605
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9739..9804
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9934..9954
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10379..10513
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10682..11315
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11362..11487
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11979..12212
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12397..12441
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcttggg ctagcagtga
      601 actccaagcc accatggatt tggccaacca tggacttatt ctactgcaac agttaaacgc
      661 tcagcgagag tttggtttcc tgtgtgactg cacggttgca atcggcgatg tatacttcaa
      721 ggcacacaaa tcagttcttg cttcattctc caattacttt aagatgttgt ttgtccatca
      781 gaccagtgag tgtgtccgct tgaaaccaac tgacatacag cccgacatct tcagctatct
      841 cttacacttg atgtacactg ggaagatggc gcctcagctc atcgacccgg ttcgattaga
      901 acagggcatc aagtttctgc acgcctaccc gctcattcag gaagccagcc tcgccagcca
      961 gggagccttt tctcaccctg accaagtttt cccactggct tcttcattgt atggcattca
     1021 gattgcagat catcagttga gacaagccac caagattgct tcagcacctg aaaaactcgg
     1081 gcgagatcca cggccacaga cctccaggat aagccaggag caggtccctg aggcctcaca
     1141 gctctcccag ctgacttcaa atctggccca ggtgaatcgg acaaatatga ctccctcaga
     1201 ccccctgcag acctcgctgt ctccagaact tgtttccact cctgttcctc cccctcctcc
     1261 cggggaggag accaatctgg aagcatcttc ctccgatgag cagcctgcgt ccctcacaat
     1321 agcccacgtc aagccaagca tcatgaagag gaatgggagc tttccaaagt actatgcctg
     1381 ccacctgtgt ggacggcgct tcactctccg gagcagctta cgtgaacacc tccagatcca
     1441 cacaggagta cctttcacat ctagccaaca gggagaaagt cgcgtccccc tgactctctg
     1501 tagcaatgca gctgacctcg ggaaagatgc catggaagtg cctgaagccg ggatgataag
     1561 tgacagtgag ctgcagcaca tctctgattc tcccatcatc gatgggcagc agcagtcgga
     1621 aaccccaccc ccctcagaca ttgctgacat tgacaacctg gagcaggccg accaggagag
     1681 ggaggtgaag aggcggaagt acgagtgcac aatatgtgga cgcaaattta tccagaaaag
     1741 ccactggagg gaacacatgt acatacacac cgggaagcct ttcaagtgca gcacttgtga
     1801 caaaagcttt tgcagggcca accaggctgc ccgccacgtg tgcctcaacc agagcatcga
     1861 cacttacacc atggtggaca aacagactct ggaactctgc acatttgagg aagggagtca
     1921 gatggacaac atgctggtgc aaacaaacaa accctacaaa tgcaacttgt gtgacaaaac
     1981 attctccact cccaatgagg ttgttaaaca ttcatgccaa aaccagaact cggatgtttt
     2041 tgccctagac gaagggcgat ccattctcct gggcagtggg gactcggaag taacggagcc
     2101 tgaccaccca gtgttagctt ccatcaaaaa ggaacaagaa accgtcttac tagactaccc
     2161 ctacgacgtg cctgactacg ccggcggcgg tggttcaggc ggtggcggtt ccggtggtgg
     2221 gggatcagtc caactggtgg agtctggtgg cgctttggtg cagccaggtg gctctctgcg
     2281 tttgtcctgt gccgcttctg gcttcccagt gaaccgctat tccatgcgct ggtatcgcca
     2341 ggctccaggc aaagagcgtg agtgggtagc cggtatgtcc agcgcgggtg atcgtagctc
     2401 ctatgaagac tccgtgaagg gccgtttcac catcagccgt gacgatgccc gtaacacggt
     2461 gtatctgcaa atgaacagct tgaaacctga agatacggcc gtgtattact gtaatgtgaa
     2521 cgtgggcttc gagtattggg gccaaggcac ccaggtcacc gtctccagct aagatccttg
     2581 acttgcggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag
     2641 tcgacctgca tctaggcgcc ggaattagat ctctcgaggt taacgaattc taccgggtag
     2701 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     2761 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     2821 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     2881 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     2941 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     3001 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     3061 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     3121 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     3181 ctccgggcct ttcgacctgc agcccaagct taccatggtg agcaagggcg aggaggataa
     3241 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3301 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3361 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     3421 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     3481 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     3541 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     3601 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     3661 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     3721 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     3781 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     3841 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     3901 cggcatggac gagctgtaca aggcaacaaa cttctctctg ctgaaacaag ccggagatgt
     3961 cgaagagaat cctggaccga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4021 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4081 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4141 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4201 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4261 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4321 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4381 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4441 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4501 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4561 ccgcaagccc ggtgcctgat cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4621 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4681 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4741 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4801 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4861 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4921 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4981 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5041 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5101 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5161 tttgggccgc ctccccgcat cgataccgtc gaccaaggca gctgtagatc ttagccactt
     5221 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5281 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5341 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5401 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5461 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     5521 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     5581 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     5641 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     5701 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     5761 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     5821 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     5881 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     5941 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     6001 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctagc tctgatgccg
     6061 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6121 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6181 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     6241 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6301 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7561 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8221 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     8281 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8341 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8401 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8461 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8521 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8581 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     8641 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     8701 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     8761 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     8821 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     8881 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     8941 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     9001 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     9061 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     9121 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     9181 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     9241 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     9301 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     9361 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     9421 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     9481 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     9541 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     9601 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
     9661 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     9721 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     9781 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     9841 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     9901 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     9961 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
    10021 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
    10081 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
    10141 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
    10201 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
    10261 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    10321 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    10381 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10441 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10501 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    10561 ataccctatt accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct
    10621 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10681 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
    10741 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
    10801 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    10861 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    10921 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    10981 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    11041 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    11101 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    11161 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    11221 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    11281 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    11341 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    11401 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    11461 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    11521 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    11581 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    11641 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    11701 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    11761 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    11821 caagcggccg gtgatcttca gacctggacg atatatatga gggacaattg gagaagtgaa
    11881 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    11941 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    12001 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    12061 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    12121 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    12181 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    12241 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    12301 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    12361 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    12421 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    12481 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    12541 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    12601 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    12661 gacagatcca ttcgattagt gaacggatct cgacggtcg
//
