LOCUS       V034509                12618 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034509
VERSION     V034509
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2074
                     /label="ZBTB32(NM_014383)"
                     /note="ZBTB32(NM_014383)"
                     /gene="ZBTB32"
     CDS             2075..2101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2147..2488
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2614..3113
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3134..3841
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3842..3898
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3899..4498
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4509..5097
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5160..5393
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5418..5436)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6225..6329
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6330..7190
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7361..7949
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8195..8524
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9658..9723
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9853..9873
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10298..10432
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10601..11234
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11281..11406
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11898..12131
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12316..12360
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctccctat aaagcagtga
      601 actccaagcc accatgtccc tgccccccat aagactgccc agcccctatg gctctgatcg
      661 gctggtacag ctagcagcca ggctccggcc agcactctgt gatactctga tcaccgtagg
      721 gagccaggag ttccccgccc acagcctggt gctagcaggt gtcagccagc agctgggccg
      781 caggggccag tgggctctgg gagaaggcat cagcccttct acctttgccc agctcctgaa
      841 ctttgtgtat ggggagagtg tagagctgca gcctggagag ctaaggcccc ttcaggaggc
      901 ggccagggcc ttgggagtgc agtccctgga agaggcatgc tggagggctc gaggggacag
      961 ggctaaaaag ccagatccag gcctgaagaa acatcaggag gagccagaga aaccctcaag
     1021 gaatcctgag agagaactgg gggaccctgg agagaagcag aaaccagaac aggtttctag
     1081 aactggtggg agagaacagg agatgttgca caagcactcg ccaccaagag gcagacccga
     1141 gatggcagga gcaacgcagg aggctcagca ggaacagacc aggtcaaagg agaaacgcct
     1201 ccaagcccct gttggccaaa ggggagcaga tgggaagcat ggagtgctca cgtggttgag
     1261 ggaaaatcca gggggctctg aggaaagtct gcgcaagctc cctggccccc ttcccccagc
     1321 aggctccctg caaaccagcg tcacccctag gccctcgtgg gctgaggccc cttggttggt
     1381 ggggggccag cctgccctgt ggagcatcct gctgatgccg cccagatatg gcattccctt
     1441 ctaccatagc acccccacca ctggagcctg gcaggaggtc tggcgggaac agaggatccc
     1501 actgtcccta aatgccccca aagggctctg gagccagaac cagttggcct cctccagccc
     1561 taccccaggt tccctccccc agggccccgc acagctcagc cctggggaga tggaagagtc
     1621 tgatcagggg cacacaggcg cacttgcaac ctgtgcgggt catgaggaca aggcaggctg
     1681 cccacctcgc ccgcaccctc ccccggcccc tcctgctcgg tctcggccct atgcgtgctc
     1741 tgtctgtgga aagaggtttt cactcaagca tcagatggag acgcactacc gagtccacac
     1801 aggagagaag cccttctcct gtagcctttg tcctcagcgc tcccgggact tctcggccat
     1861 gaccaagcac ctgcggacac acggggccgc tccgtaccgc tgctccctgt gcggggccgg
     1921 ctgtcccagc ctggcctcca tgcaggcgca catgcgcggt cactcgccca gccaactccc
     1981 gcccggatgg accatccgct ccaccttcct ctactcctcc tcgaggccgt ctcggccctc
     2041 gacctctccc tgttgtcctt cttcctccac cacctacccc tacgacgtgc ctgactacgc
     2101 cggcggcggt ggttcaggcg gtggcggttc cggtggtggg ggatcagtcc aactggtgga
     2161 gtctggtggc gctttggtgc agccaggtgg ctctctgcgt ttgtcctgtg ccgcttctgg
     2221 cttcccagtg aaccgctatt ccatgcgctg gtatcgccag gctccaggca aagagcgtga
     2281 gtgggtagcc ggtatgtcca gcgcgggtga tcgtagctcc tatgaagact ccgtgaaggg
     2341 ccgtttcacc atcagccgtg acgatgcccg taacacggtg tatctgcaaa tgaacagctt
     2401 gaaacctgaa gatacggccg tgtattactg taatgtgaac gtgggcttcg agtattgggg
     2461 ccaaggcacc caggtcaccg tctccagcta agatccttga cttgcggccg caactcccac
     2521 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcat ctaggcgccg
     2581 gaattagatc tctcgaggtt aacgaattct accgggtagg ggaggcgctt ttcccaaggc
     2641 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     2701 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     2761 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     2821 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     2881 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     2941 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     3001 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     3061 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     3121 gcccaagctt accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3181 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3241 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3301 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3361 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3421 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3481 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3541 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     3601 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3661 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     3721 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     3781 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3841 ggcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat
     3901 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3961 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4021 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4081 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4141 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4201 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4261 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4321 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4381 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4441 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     4501 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4561 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4621 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4681 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4741 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4801 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4861 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4921 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4981 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5041 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5101 gataccgtcg accaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5161 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5221 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5281 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5341 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     5401 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     5461 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     5521 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     5581 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     5641 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     5701 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     5761 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     5821 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     5881 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     5941 cgaatggtgc actctcagta caatctagct ctgatgccgc atagttaagc cagccccgac
     6001 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6061 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6121 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6181 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6301 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6361 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6421 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6481 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6541 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6601 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6661 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6721 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6781 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6841 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6901 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6961 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7021 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7081 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7141 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7201 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7261 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7321 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7381 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7441 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7501 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7561 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7621 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7681 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7741 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7801 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7861 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7921 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7981 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8041 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8101 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8161 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8221 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8341 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8401 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8461 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8521 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     8581 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8641 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     8701 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     8761 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     8821 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     8881 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     8941 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     9001 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     9061 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     9121 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9181 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9241 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9301 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9361 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9421 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     9481 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     9541 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9601 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9661 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     9721 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     9781 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     9841 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     9901 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     9961 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    10021 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    10081 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    10141 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10201 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10261 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10441 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10501 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10561 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    10621 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    10681 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    10741 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    10801 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    10861 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    10921 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    10981 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    11041 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11101 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11161 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11221 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    11281 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    11341 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    11401 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    11461 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    11521 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    11581 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    11641 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    11701 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg tgatcttcag
    11761 acctggacga tatatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    11821 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    11881 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    11941 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    12001 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    12061 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    12121 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    12181 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    12241 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    12301 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    12361 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    12421 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    12481 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    12541 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    12601 aacggatctc gacggtcg
//
