LOCUS V018280 9337 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018280 VERSION V018280 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2809..2818 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2818..2847 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2854..4020 /label="Kctd9(NM_001108871)" /note="Kctd9(NM_001108871)" /gene="Kctd9" misc_feature 4040..4626 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 4646..5245 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5259..5847 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6054..6687 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6815..6831) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6839..6855 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6863..6893) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6908..6929 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7217..7805) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7976..8836) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8837..8941) /gene="bla" /label="AmpR promoter" polyA_signal 8989..9123 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa 2821 caaaaactca tctcagaaga ggatctgctc gagatgcggc gggtcacgct gttcttgaac 2881 ggcagcccga ggaacggcaa ggtggttgct gtatacggaa ctttgtctga tttgctctca 2941 gtagccagca gtaaactcgg cataaaagcc accagtgtat ataatgggaa aggcggactg 3001 attgatgata ttgctttgat cagggatgat gatgtgttgt ttgtgtgtga aggagaacca 3061 tttattgatc ctcagacaga tgccagaccg cctggaggat tgctaggatc tcacacagac 3121 tggcttacat tgaatgtagg agggcgatac tttacaacaa caaggagcac tttagtgaat 3181 aaagagcctg acagtatgct ggcgcacatg ttcaaggaca aaggtgtctg gggaaataag 3241 caagatcata gaggagcttt cttaattgac cgaagtcctg agtactttga acctattttg 3301 aactatttac gtcatggaca gctcattgta aatgatggca ttaacttatt gggtgttcta 3361 gaagaagcca ggttttttgg tattgactca ctgattgaac acctagaagt ggccataaag 3421 aattcccaac caccagagga tcattcacca atatcccgaa aggaatttgt tcgatttctg 3481 ctggccactc caaccaagtc agagctgcgc tgccagggtt taaacttcag tggtgctgat 3541 ctttcccgtt tggaccttcg atacattaac ttcaaaatgg ccaatttaag ccgctgcaac 3601 ctcgcacatg caaatctctg ctgtgctaat cttgaacgag ctgatctttc tggatcagtg 3661 ctcgactgtg caaatctcca gggagtcaag atgctctgct caaacgcaga aggtgcatcc 3721 ctgaggctct gtaattttga ggatccttca ggtcttaaag ccaacttaga gggtgctaat 3781 ctgaaaggtg tggacatgga aggaagccag atgacaggga ttaacctcag agttgctacc 3841 ttaaaaaatg cgaaactgaa gaactgtaat ctcagaggag caactctggc aggaacagat 3901 ttagaaaact gtgatttgtc ggggtgtgac ctccaggaag ccaacttgag aggctccaac 3961 gtgaagggtg ccatatttga agagatgctg acaccattac atatgtctca gagtgtcaga 4021 tgagcggccg cggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc 4081 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 4141 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc 4201 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc 4261 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc 4321 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg 4381 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 4441 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat 4501 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc 4561 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc 4621 acaacccaca aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac 4681 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 4741 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 4801 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 4861 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 4921 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 4981 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 5041 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 5101 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 5161 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 5221 catgacccgc aagcccggtg cctagacgcg tctggaacaa tcaacctctg gattacaaaa 5281 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 5341 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 5401 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 5461 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 5521 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 5581 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 5641 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 5701 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 5761 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 5821 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa 5881 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga 5941 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat 6001 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg 6061 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg 6121 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt 6181 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg 6241 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga 6301 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca 6361 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga 6421 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat 6481 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg 6541 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 6601 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 6661 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 6721 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac 6781 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 6841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6961 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 7021 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 7681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 7921 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 7981 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 8041 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 8101 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 8161 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 8221 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 8281 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 8341 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8401 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8461 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8521 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8581 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8641 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8701 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8761 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8821 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 8881 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8941 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt 9001 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 9061 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 9121 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 9181 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt 9241 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 9301 attgtatttg ttaaatatgt actacaaact tagtagt //