LOCUS       V021071                10938 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021071
VERSION     V021071
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..5598
                     /label="RB1(NM_000321)"
                     /note="RB1(NM_000321)"
                     /gene="RB1"
     CDS             5599..5622
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     misc_feature    5641..6227
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             6247..6846
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6860..7448
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7655..8288
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8416..8432)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8440..8456
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8464..8494)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8509..8530
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8818..9406)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9577..10437)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10438..10542)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10590..10724
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgccg
     2821 cccaaaaccc cccgaaaaac ggccgccacc gccgccgctg ccgccgcgga acccccggca
     2881 ccgccgccgc cgccccctcc tgaggaggac ccagagcagg acagcggccc ggaggacctg
     2941 cctctcgtca ggcttgagtt tgaagaaaca gaagaacctg attttactgc attatgtcag
     3001 aaattaaaga taccagatca tgtcagagag agagcttggt taacttggga gaaagtttca
     3061 tctgtggatg gagtattggg aggttatatt caaaagaaaa aggaactgtg gggaatctgt
     3121 atctttattg cagcagttga cctagatgag atgtcgttca cttttactga gctacagaaa
     3181 aacatagaaa tcagtgtcca taaattcttt aacttactaa aagaaattga taccagtacc
     3241 aaagttgata atgctatgtc aagactgttg aagaagtatg atgtattgtt tgcactcttc
     3301 agcaaattgg aaaggacatg tgaacttata tatttgacac aacccagcag ttcgatatct
     3361 actgaaataa attctgcatt ggtgctaaaa gtttcttgga tcacattttt attagctaaa
     3421 ggggaagtat tacaaatgga agatgatctg gtgatttcat ttcagttaat gctatgtgtc
     3481 cttgactatt ttattaaact ctcacctccc atgttgctca aagaaccata taaaacagct
     3541 gttataccca ttaatggttc acctcgaaca cccaggcgag gtcagaacag gagtgcacgg
     3601 atagcaaaac aactagaaaa tgatacaaga attattgaag ttctctgtaa agaacatgaa
     3661 tgtaatatag atgaggtgaa aaatgtttat ttcaaaaatt ttataccttt tatgaattct
     3721 cttggacttg taacatctaa tggacttcca gaggttgaaa atctttctaa acgatacgaa
     3781 gaaatttatc ttaaaaataa agatctagat gcaagattat ttttggatca tgataaaact
     3841 cttcagactg attctataga cagttttgaa acacagagaa caccacgaaa aagtaacctt
     3901 gatgaagagg tgaatgtaat tcctccacac actccagtta ggactgttat gaacactatc
     3961 caacaattaa tgatgatttt aaattcagca agtgatcaac cttcagaaaa tctgatttcc
     4021 tattttaaca actgcacagt gaatccaaaa gaaagtatac tgaaaagagt gaaggatata
     4081 ggatacatct ttaaagagaa atttgctaaa gctgtgggac agggttgtgt cgaaattgga
     4141 tcacagcgat acaaacttgg agttcgcttg tattaccgag taatggaatc catgcttaaa
     4201 tcagaagaag aacgattatc cattcaaaat tttagcaaac ttctgaatga caacattttt
     4261 catatgtctt tattggcgtg cgctcttgag gttgtaatgg ccacatatag cagaagtaca
     4321 tctcagaatc ttgattctgg aacagatttg tctttcccat ggattctgaa tgtgcttaat
     4381 ttaaaagcct ttgattttta caaagtgatc gaaagtttta tcaaagcaga aggcaacttg
     4441 acaagagaaa tgataaaaca tttagaacga tgtgaacatc gaatcatgga atcccttgca
     4501 tggctctcag attcaccttt atttgatctt attaaacaat caaaggaccg agaaggacca
     4561 actgatcacc ttgaatctgc ttgtcctctt aatcttcctc tccagaataa tcacactgca
     4621 gcagatatgt atctttctcc tgtaagatct ccaaagaaaa aaggttcaac tacgcgtgta
     4681 aattctactg caaatgcaga gacacaagca acctcagcct tccagaccca gaagccattg
     4741 aaatctacct ctctttcact gttttataaa aaagtgtatc ggctagccta tctccggcta
     4801 aatacacttt gtgaacgcct tctgtctgag cacccagaat tagaacatat catctggacc
     4861 cttttccagc acaccctgca gaatgagtat gaactcatga gagacaggca tttggaccaa
     4921 attatgatgt gttccatgta tggcatatgc aaagtgaaga atatagacct taaattcaaa
     4981 atcattgtaa cagcatacaa ggatcttcct catgctgttc aggagacatt caaacgtgtt
     5041 ttgatcaaag aagaggagta tgattctatt atagtattct ataactcggt cttcatgcag
     5101 agactgaaaa caaatatttt gcagtatgct tccaccaggc cccctacctt gtcaccaata
     5161 cctcacattc ctcgaagccc ttacaagttt cctagttcac ccttacggat tcctggaggg
     5221 aacatctata tttcacccct gaagagtcca tataaaattt cagaaggtct gccaacacca
     5281 acaaaaatga ctccaagatc aagaatctta gtatcaattg gtgaatcatt cgggacttct
     5341 gagaagttcc agaaaataaa tcagatggta tgtaacagcg accgtgtgct caaaagaagt
     5401 gctgaaggaa gcaaccctcc taaaccactg aaaaaactac gctttgatat tgaaggatca
     5461 gatgaagcag atggaagtaa acatctccca ggagagtcca aatttcagca gaaactggca
     5521 gaaatgactt ctactcgaac acgaatgcaa aagcagaaaa tgaatgatag catggatacc
     5581 tcaaacaagg aagagaaaga ttacaaggac gacgatgaca agtagcggcc gcggatcccg
     5641 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     5701 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5761 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5821 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     5881 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     5941 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     6001 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     6061 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     6121 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     6181 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga
     6241 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
     6301 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
     6361 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6421 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6481 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6541 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6601 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6661 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6721 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6781 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6841 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6901 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6961 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7021 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7081 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7141 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7201 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7261 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7321 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7381 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7441 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     7501 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7561 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7621 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     7681 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     7741 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     7801 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     7861 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     7921 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     7981 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     8041 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     8101 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8161 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8221 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8281 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8341 aatatcagag agtgagaggc cttgacattg ctagcgttta ccgtcgacct ctagctagag
     8401 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8461 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8521 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8581 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8641 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8701 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8761 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8821 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8881 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8941 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9001 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9061 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9121 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9181 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9241 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9301 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9361 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9421 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9481 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9541 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9601 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9661 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9721 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9781 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9841 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9901 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9961 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10021 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10081 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10141 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10201 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10261 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10321 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10381 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10441 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10501 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10561 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
    10621 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10681 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10741 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10801 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10861 tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg
    10921 tactacaaac ttagtagt
//
