LOCUS       V022509                10582 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022509
VERSION     V022509
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2809..2818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2818..2847
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2854..5259
                     /label="USP10(NM_001272075)"
                     /note="USP10(NM_001272075)"
                     /gene="USP10"
     misc_feature    5285..5871
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5891..6490
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6504..7092
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7299..7932
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8060..8076)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8084..8100
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8108..8138)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8153..8174
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8462..9050)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9221..10081)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10082..10186)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10234..10368
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa
     2821 caaaaactca tctcagaaga ggatctgctc gagatgccct ggttgccctc tcctggaata
     2881 gggcagtata tttttggaga ttttagccct gatgaattca atcaattctt tgtgactcct
     2941 cgatcttcag ttgagcttcc tccatacagt ggaacagttc tgtgtggcac acaggctgtg
     3001 gataaactac ctgatggaca agaatatcag agaattgagt ttggtgtcga tgaagtcatt
     3061 gaacccagtg acactttgcc gagaaccccc agctacagta tttcaagcac actgaaccct
     3121 caggcccctg aatttattct cggttgtaca gcttccaaaa taacccctga tggtatcact
     3181 aaagaagcaa gctatggctc catcgactgc cagtacccag gctctgccct cgctttggat
     3241 ggaagttcta atgtggaggc ggaagttttg gaaaatgatg gtgtctcagg tggtcttgga
     3301 caaagggagc gtaaaaagaa gaaaaagcgg ccacctggat attacagcta tttgaaagat
     3361 ggtggcgatg atagtatctc cacagaagcc ctggtcaatg gccatgccaa ttcagcagtc
     3421 ccgaacagtg tcagtgcaga ggatgcagaa tttatgggtg acatgccccc gtcagttacg
     3481 cccaggactt gtaacagccc ccagaactcc acagactctg tcagtgacat tgtgcctgac
     3541 agtcctttcc ccggagcact cggcagtgac accaggactg cagggcagcc agaggggggc
     3601 cccggggctg attttggtca gtcctgcttc cctgcagagg ctggcagaga caccctgtca
     3661 aggacagctg gggctcagcc ctgcgttggt accgatacta ctgaaaacct tggagttgct
     3721 aatggacaaa tacttgaatc ctcgggtgag ggcacagcta ccaacggggt ggagttgcac
     3781 accacggaaa gcatagactt ggacccaacc aaacccgaga gtgcatcacc tcctgctgac
     3841 ggcacgggct ctgcatcagg cacccttcct gtcagccagc ccaagtcctg ggccagcctc
     3901 tttcatgatt ctaagccctc ttcctcctcg ccggtggcct atgtggaaac taagtattcc
     3961 cctcccgcca tatctcccct ggtttctgaa aagcaggttg aagtcaaaga agggcttgtt
     4021 ccggtttcag aggatcctgt agccataaag attgcagagt tgctggagaa tgtaacccta
     4081 atccataaac cagtgtcgtt gcaaccccgt gggctgatca ataaagggaa ctggtgctac
     4141 attaatgcta cactgcaggc attggttgct tgcccgccga tgtaccacct gatgaagttc
     4201 attcctctgt attccaaagt gcaaaggcct tgtacgtcaa cacccatgat agacagcttt
     4261 gttcggctaa tgaatgagtt cactaatatg ccagtacctc caaaaccccg acaagctctt
     4321 ggagataaaa tcgtgaggga tattcgccct ggagctgcct ttgagcccac atatatttac
     4381 agactcctga cagttaacaa gtcaagcctg tctgaaaagg gtcgacaaga agatgctgag
     4441 gaatacttag gcttcattct aaatggactt catgaggaaa tgttgaacct aaagaagctt
     4501 ctctcaccaa gtaatgaaaa acttacgatt tccaacggcc ccaaaaacca ctcggtcaat
     4561 gaagaagagc aggaagaaca aggtgaagga agcgaggatg aatgggaaca agtgggcccc
     4621 cggaacaaga cttccgtcac ccgccaggcg gattttgttc agactccaat caccggcatt
     4681 tttggtggac acatcaggtc tgtggtttac cagcagagtt caaaagaatc tgccactttg
     4741 cagccatttt tcacgttgca gttggatatc cagtcagaca agatacgcac agtccaggat
     4801 gcactggaga gcttggtggc aagagaatct gtccaaggtt ataccacaaa aaccaaacaa
     4861 gaggttgaga taagtcgaag agtgactctg gaaaaactcc ctcctgtcct cgtgctgcac
     4921 ctgaaacgat tcgtttatga gaagactggt gggtgccaga agcttatcaa aaatattgaa
     4981 tatcctgtgg acttggaaat tagtaaagaa ctgctttctc caggggttaa aaataagaat
     5041 tttaaatgcc accgaaccta tcggctcttt gcagtggtct accatcacgg caacagtgcg
     5101 acgggcggcc attacactac agacgtcttc cagatcggtc tgaatggctg gctgcgcatc
     5161 gatgaccaga cagtcaaggt gatcaaccag taccaggtgg tgaaaccaac tgctgaacgc
     5221 acagcctacc tcctgtatta ccgccgagtg gacctgctgt aatctagagc ggccgcggat
     5281 cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5341 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5401 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5461 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5521 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5581 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5641 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5701 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5761 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5821 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag
     5881 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5941 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6001 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6061 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6121 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6181 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6241 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6301 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6361 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6421 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6481 cggtgcctag acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     6541 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6601 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6661 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6721 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6781 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6841 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6901 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6961 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7021 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7081 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
     7141 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7201 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     7261 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
     7321 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     7381 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     7441 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     7501 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     7561 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     7621 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     7681 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     7741 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7801 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7861 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7921 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7981 aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct
     8041 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8101 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8161 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8221 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8281 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8341 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8401 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8461 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8521 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8581 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8641 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8701 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8761 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8821 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8881 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8941 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9001 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9061 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9121 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9181 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9241 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9301 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9361 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9421 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9481 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9541 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9601 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9661 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9721 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9781 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9841 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9901 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9961 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10021 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10081 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10141 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10201 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
    10261 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10321 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    10381 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    10441 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    10501 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa
    10561 tatgtactac aaacttagta gt
//
