LOCUS       V038811                12458 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038811
VERSION     V038811
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2337..3519
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     CDS             3548..7180
                     /EC_number=""
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="precursor of human epidermal growth factor
                     receptor"
                     /protein_id=""
                     /transl_table=1
                     /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF
                     EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN
                     LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI
                     QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG
                     RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN
                     PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG
                     IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV
                     KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS
                     DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW
                     GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG
                     PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE
                     GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP
                     LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA
                     TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI
                     GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKE
                     YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL
                     EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER
                     MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS
                     TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS
                     VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH
                     QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA*"
                     /label="EGFR"
     misc_feature    7208..7794
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             7814..8413
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8427..9015
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9222..9855
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9983..9999)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10007..10023
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10031..10061)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10076..10097
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10385..10973)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11144..12004)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12005..12109)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12157..12291
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg cgaccctccg ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct
     3601 ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc caaggcacga gtaacaagct
     3661 cacgcagttg ggcacttttg aagatcattt tctcagcctc cagaggatgt tcaataactg
     3721 tgaggtggtc cttgggaatt tggaaattac ctatgtgcag aggaattatg atctttcctt
     3781 cttaaagacc atccaggagg tggctggtta tgtcctcatt gccctcaaca cagtggagcg
     3841 aattcctttg gaaaacctgc agatcatcag aggaaatatg tactacgaaa attcctatgc
     3901 cttagcagtc ttatctaact atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag
     3961 aaatttacag gaaatcctgc atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa
     4021 cgtggagagc atccagtggc gggacatagt cagcagtgac tttctcagca acatgtcgat
     4081 ggacttccag aaccacctgg gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag
     4141 ctgctggggt gcaggagagg agaactgcca gaaactgacc aaaatcatct gtgcccagca
     4201 gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc
     4261 aggctgcaca ggcccccggg agagcgactg cctggtctgc cgcaaattcc gagacgaagc
     4321 cacgtgcaag gacacctgcc ccccactcat gctctacaac cccaccacgt accagatgga
     4381 tgtgaacccc gagggcaaat acagctttgg tgccacctgc gtgaagaagt gtccccgtaa
     4441 ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat
     4501 ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa
     4561 cggaataggt attggtgaat ttaaagactc actctccata aatgctacga atattaaaca
     4621 cttcaaaaac tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg
     4681 tgactccttc acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt
     4741 aaaggaaatc acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca
     4801 tgcctttgag aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct
     4861 tgcagtcgtc agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga
     4921 tggagatgtg ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa
     4981 aaaactgttt gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag
     5041 ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc
     5101 ggagcccagg gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa
     5161 gtgcaacctt ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg
     5221 ccacccagag tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa
     5281 ctgtatccag tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg
     5341 agtcatggga gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca
     5401 cctgtgccat ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac
     5461 gaatgggcct aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct
     5521 ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac
     5581 gctgcggagg ctgctgcagg agagggagct tgtggagcct cttacaccca gtggagaagc
     5641 tcccaaccaa gctctcttga ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct
     5701 gggctccggt gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt
     5761 taaaattccc gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga
     5821 aatcctcgat gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct
     5881 gggcatctgc ctcacctcca ccgtgcagct catcacgcag ctcatgccct tcggctgcct
     5941 cctggactat gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg
     6001 tgtgcagatc gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacct
     6061 ggcagccagg aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggag
     6121 ggccaaactg ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat
     6181 caagtggatg gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg
     6241 gagctacggg gtgactgttt gggagttgat gacctttgga tccaagccat atgacggaat
     6301 ccctgccagc gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat
     6361 atgtaccatc gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg
     6421 cccaaagttc cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta
     6481 ccttgtcatt cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta
     6541 ccgtgccctg atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat
     6601 cccacagcag ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct
     6661 gagtgcaacc agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg
     6721 tcccatcaag gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac
     6781 tgaggacagc atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc
     6841 caaaaggccc gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc
     6901 gcccagcaga gacccacact accaggaccc ccacagcact gcagtgggca accccgagta
     6961 tctcaacact gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc
     7021 ccagaaaggc agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc
     7081 caaggaagcc aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct
     7141 aagggtcgcg ccacaaagca gtgaatttat tggagcatga ctagttctag agcggccgcg
     7201 gatcccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     7261 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     7321 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     7381 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     7441 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     7501 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     7561 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     7621 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     7681 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     7741 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccacaag
     7801 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     7861 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7921 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     7981 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     8041 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8101 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8161 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     8221 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     8281 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     8341 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     8401 gcccggtgcc tagacgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     8461 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     8521 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     8581 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     8641 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8701 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     8761 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     8821 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8881 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8941 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9001 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     9061 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     9121 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     9181 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     9241 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     9301 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     9361 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     9421 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     9481 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     9541 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     9601 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     9661 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9721 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9781 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9841 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9901 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta
     9961 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10021 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10081 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10141 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10561 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    10741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    10801 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    10861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    10921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    10981 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11041 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11101 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11161 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11221 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11281 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11341 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11401 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11461 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11521 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11581 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11641 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    11701 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    11761 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11821 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    11881 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    11941 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12001 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    12061 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    12121 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
    12181 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    12241 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    12301 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    12361 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    12421 aattgtattt gttaaatatg tactacaaac ttagtagt
//
