LOCUS       V037917                10402 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037917
VERSION     V037917
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5256
                     /label="AARS2(NM_020745)"
                     /note="AARS2(NM_020745)"
                     /gene="AARS2"
     CDS             5263..5328
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5372..5583
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5596..5864
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5891..6490
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6497..7085
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7159..7392
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7464..7585
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7604..7739
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7777..7793)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7801..7817
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7825..7855)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7870..7891
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8179..8767)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8938..9798)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9799..9903)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10377..10393
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcagcg tcagtggcag ctgcagcccg gaggctgcgg
     2341 cgggccattc gaaggtcgcc cgcatggcgg ggcctcagcc atcggccgct ctcatcggag
     2401 ccccctgcag ccaaggcctc ggccgtgagg gccgcctttc tgaacttctt tcgggaccgc
     2461 catggccacc ggctggtgcc ctccgcttcc gtgcggcccc gcggcgaccc cagtttgctt
     2521 tttgtcaatg cgggcatgaa ccagttcaag ccaatctttc tgggcaccgt ggatccacga
     2581 agcgagatgg caggcttccg acgtgtggcc aacagccaga aatgtgtgag agctggagga
     2641 caccataacg acctggaaga tgtgggtcga gacctttccc atcatacctt ctttgaaatg
     2701 cttggcaatt gggcctttgg gggtgaatat tttaaggagg aggcttgtaa catggcctgg
     2761 gaactgctga ctcaggtcta tgggatccct gaggaaaggc tctggatctc ctactttgat
     2821 ggtgacccca aggcagggct ggacccagac ctggagacca gggacatctg gctgagctta
     2881 ggggtgcctg ctagccgtgt gctttccttt ggaccacaag agaacttctg ggagatgggg
     2941 gatactggcc cttgtgggcc ctgtactgag atccactacg accttgctgg tggggtggga
     3001 gccccccagc tggtagagct ttggaacctg gtcttcatgc aacacaacag agaggcagat
     3061 ggaagcctgc agcccctgcc ccagcggcat gtggacacag gaatgggcct ggaaaggctg
     3121 gtggctgtgc tgcaaggcaa acactccacc tatgacactg acctcttttc cccgctgctc
     3181 aacgccatac agcagggctg cagggcaccc ccttacttgg gccgagtagg ggtggcagac
     3241 gaggggcgca cagacacagc gtaccgcgtg gtggctgacc acatccgcac actcagtgtc
     3301 tgcatctctg atggcatctt ccctgggatg tcaggtcccc cgctggttct tcgtcggatc
     3361 ctgcgtcgag ctgtgcgttt ctccatggag atcttaaagg caccacctgg cttcctaggc
     3421 agcctggtac ctgtagtggt ggagacactg ggagatgctt atccagaact gcaaaggaac
     3481 tcagcccaga tcgccaacct ggtgtcagag gacgaggcag ccttcctggc ctccctggag
     3541 cggggtaggc ggatcattga tcggactctg aggaccctgg ggccttcaga tatgttccct
     3601 gctgaagtgg cctggtcctt gtcactgtgt ggagacctgg gactcccctt ggacatggta
     3661 gagctgatgc tggaggagaa aggggtccag ctagactccg ctggactgga gcggttggcc
     3721 caagaggagg cccagcaccg ggcacggcag gctgagccag ttcagaagca gggattgtgg
     3781 cttgatgtcc atgcgcttgg ggagctgcag cgccaaggag tgcccccaac tgacgacagc
     3841 cccaagtaca actactccct gcgacccagc ggaagttatg agttcggcac ctgtgaggcc
     3901 caggtgttgc aactgtatac agaggacggg acagcagtgg cctccgtggg gaaaggccag
     3961 cgctgtggcc tcctcttgga caggaccaac ttctacgcag aacagggggg ccaggcttca
     4021 gaccgtggct acctggtgcg ggcagggcaa gaggacgtgc tgttcccagt agcccgggcc
     4081 caggtctgtg gaggtttcat cctgcatgag gcagtagccc ctgagtgcct gcggttaggg
     4141 gaccaggtgc agctgcatgt ggatgaggcc tggcgtctag gctgcatggc gaagcatacg
     4201 gccacccacc tgctgaactg ggcactgagg cagaccctgg gccctggcac agagcagcag
     4261 ggctcccatc tcaatcctga gcagctgcgc ttggatgtga ccacccagac cccattgacc
     4321 ccagagcagc tccgggcagt ggagaacact gtgcaggagg ccgtggggca ggatgaggct
     4381 gtgtacatgg aggaggtgcc cctggcgctc actgcccagg tccctggcct gcgctctctg
     4441 gatgaggttt acccagaccc tgtgcgggtg gtatcagtgg gggtgcccgt ggcccatgca
     4501 ttggacccag cctcccaagc cgcactgcag acctctgtgg agctatgctg tgggacgcac
     4561 ctgttacgta ctggggctgt aggggacctg gttatcatcg gggaccgcca gctttccaag
     4621 ggcactaccc gcctgctggc cgtcactggg gagcaggccc agcaggcccg agagctaggc
     4681 cagagcctgg cccaggaagt gaaagcggcc actgagcggc tgagtctggg gagccgggat
     4741 gtggcggagg cactgaggct gtccaaggac ataggacgac tcattgaagc tgtggaaact
     4801 gctgtgatgc cccagtggca gcggcgggag ctgctggcca cagtgaagat gctgcagcgg
     4861 cgtgccaaca ctgccatccg taagctgcaa atgggacagg ctgcaaagaa aactcaggag
     4921 ctgctggagc ggcactcgaa ggggcctctg attgtggaca cagtctctgc tgagtctctc
     4981 tcagtgctgg tgaaggtggt acggcagctg tgtgagcagg cccccagcac gtctgtgctc
     5041 ctactcagcc cccagcccat ggggaaggtg ctgtgtgcct gtcaggtggc ccagggtgcc
     5101 atgcccacct tcacagcaga ggcctgggca ctggcagtgt gcagccacat ggggggcaag
     5161 gcgtggggct cacgagtggt ggcccaaggc accggaagca ctactgacct ggaagctgcc
     5221 ctcagtatag cccaaaccta tgccctcagc cagctcggat ccgactacaa agaccatgac
     5281 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     5341 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5401 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     5461 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5521 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5581 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5641 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5701 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5761 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5821 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5881 cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5941 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6001 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6061 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6121 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6181 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6241 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6301 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6361 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6421 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6481 cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6901 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7081 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7141 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt
     7201 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7261 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7321 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7381 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7441 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7501 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7561 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     7621 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7681 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7741 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     7801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8581 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9901 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9961 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10021 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10081 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10141 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10201 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10261 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10321 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10381 aacgacggcc agtgccaagc tg
//
