LOCUS       V032565                11023 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032565
VERSION     V032565
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5049
                     /label="ACTN4(NM_004924)"
                     /note="ACTN4(NM_004924)"
                     /gene="ACTN4"
     CDS             5056..5121
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5165..5376
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5389..5657
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5686..5695
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5716..6378
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6448..6501
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6502..7101
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7102..7690
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7764..7997
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8069..8190
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8230..8365
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8398..8414)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8422..8438
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8446..8476)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8491..8512
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8800..9388)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9559..10419)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10420..10524)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10998..11014
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tggactacca cgcggcgaac
     2341 cagtcgtacc agtacggccc cagcagcgcg ggcaatggcg ctggcggcgg gggcagcatg
     2401 ggcgactaca tggcccagga ggacgactgg gaccgggacc tgctgctgga cccggcctgg
     2461 gagaagcagc agcgcaagac cttcacggca tggtgcaact cccacctgcg gaaggcaggc
     2521 acacagatcg agaacattga tgaggacttc cgagacgggc tcaagctcat gctgctcctg
     2581 gaggtcatat caggggagcg gttacctaag ccggagcggg ggaagatgag agtgcacaaa
     2641 atcaacaatg tgaacaaagc gctggacttt attgccagca aaggcgtcaa gctggtctcc
     2701 atcggggcag aagagattgt ggacggcaac gcaaagatga ccctgggaat gatctggacc
     2761 atcatcctta ggttcgccat ccaggacatc tccgtggaag agacctcggc caaggaaggg
     2821 ctccttctct ggtgccagag aaagacagcc ccgtataaga acgtcaatgt gcagaacttc
     2881 cacatcagct ggaaggatgg tcttgccttc aatgccctga tccaccggca cagaccagag
     2941 ctgattgagt atgacaagct gaggaaggac gaccctgtca ccaacctgaa caatgccttc
     3001 gaagtggctg agaaatacct cgacatcccc aagatgctgg atgcagagga catcgtgaac
     3061 acggcccggc ccgacgagaa ggccataatg acctatgtgt ccagcttcta ccatgccttt
     3121 tcaggagcgc agaaggctga aactgccgcc aaccggatct gtaaggtgct ggctgtcaac
     3181 caagagaacg agcacctgat ggaggactac gagaagctgg ccagcgacct cctggagtgg
     3241 atccggcgca ccatcccctg gctggaggac cgtgtgcccc aaaagactat ccaggagatg
     3301 cagcagaagc tggaggactt ccgcgactac cggcgtgtgc acaagccgcc caaggtgcag
     3361 gagaagtgcc agctggagat caacttcaac acgctgcaga ccaagctgcg cctcagcaac
     3421 cggcccgcct tcatgccctc cgagggcaag atggtctcgg acatcaacaa tggctggcag
     3481 cacttggagc aggctgagaa gggctacgag gagtggctgc tgaatgagat ccgcaggctg
     3541 gagcggctcg accacctggc agagaagttc cggcagaagg cctccatcca cgaggcctgg
     3601 actgacggga aggaagccat gctgaagcac cgggactacg agacggccac actatcggac
     3661 atcaaagccc tcattcgcaa gcacgaggcc ttcgagagcg acctggctgc gcaccaggac
     3721 cgcgtggagc agatcgccgc cattgcccag gagctcaacg agctggatta ctacgactcc
     3781 cacaatgtca acacccggtg ccagaagatc tgtgaccagt gggacgccct cggctctctg
     3841 acacatagtc gcagggaagc cctggagaaa acagagaagc agctggaggc catcgaccag
     3901 ctgcacctgg aatacgccaa gcgcgcggcc cccttcaaca actggatgga gagcgccatg
     3961 gaggacctcc aggacatgtt catcgtccat accatcgagg agattgaggg cctgatctca
     4021 gcccatgacc agttcaagtc caccctgccg gacgccgata gggagcgcga ggccatcctg
     4081 gccatccaca aggaggccca gaggatcgct gagagcaacc acatcaagct gtcgggcagc
     4141 aacccctaca ccaccgtcac cccgcaaatc atcaactcca agtgggagaa ggtgcagcag
     4201 ctggtgccaa aacgggacca tgccctcctg gaggagcaga gcaagcagca gtccaacgag
     4261 cacctgcgcc gccagttcgc cagccaggcc aatgttgtgg ggccctggat ccagaccaag
     4321 atggaggaga tcgggcgcat ctccattgag atgaacggga ccctggagga ccagctgagc
     4381 cacctgaagc agtatgaacg cagcatcgtg gactacaagc ccaacctgga cctgctggag
     4441 cagcagcacc agctcatcca ggaggccctc atcttcgaca acaagcacac caactatacc
     4501 atggagcaca tccgcgtggg ctgggagcag ctgctcacca ccattgcccg caccatcaac
     4561 gaggtggaga accagatcct cacccgcgac gccaagggca tcagccagga gcagatgcag
     4621 gagttccggg cgtccttcaa ccacttcgac aaggatcatg gcggggcgct ggggcccgag
     4681 gagttcaagg cctgcctcat cagcctgggc tacgacgtgg agaacgaccg gcagggtgag
     4741 gccgagttca accgcatcat gagcctggtc gaccccaacc atagcggcct tgtgaccttc
     4801 caagccttca tcgacttcat gtcgcgggag accaccgaca cggacacggc tgaccaggtc
     4861 atcgcttcct tcaaggtctt agcaggggac aagaacttca tcacagctga ggagctgcgg
     4921 agagagctgc cccccgacca ggccgagtac tgcatcgccc gcatggcgcc ataccagggc
     4981 cctgacgccg tgcccggtgc cctcgactac aagtccttct ccacggcctt gtatggcgag
     5041 agcgacctgg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     5101 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5161 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5221 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5281 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5341 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5401 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5461 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5521 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5581 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5641 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     5701 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     5761 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     5821 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     5881 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     5941 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     6001 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     6061 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     6121 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     6181 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     6241 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6301 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6361 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     6421 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     6481 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6541 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6601 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6661 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6721 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6781 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6841 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6901 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6961 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7021 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7081 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     7141 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7201 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7261 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7321 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7381 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7441 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7501 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7561 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7621 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7681 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7741 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     7801 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7861 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7921 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7981 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8041 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8101 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8161 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8221 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8281 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8341 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     8401 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8461 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8521 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8581 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8641 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8701 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8761 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9241 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10561 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10621 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10681 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10741 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10801 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    10861 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10921 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10981 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
