LOCUS       V033454                10948 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033454
VERSION     V033454
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4974
                     /label="ADGRE1(NM_001974)"
                     /note="ADGRE1(NM_001974)"
                     /gene="ADGRE1"
     CDS             4981..5046
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5090..5301
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5314..5582
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5611..5620
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5641..6303
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6373..6426
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6427..7026
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7027..7615
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7689..7922
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7994..8115
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8155..8290
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8323..8339)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8347..8363
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8371..8401)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8416..8437
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8725..9313)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9484..10344)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10345..10449)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10923..10939
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc gtggcttcaa cctgctcctc
     2341 ttctggggat gttgtgttat gcacagctgg gaagggcaca taagacccac acggaaacca
     2401 aacacaaagg gtaataactg tagagacagt accttgtgcc cagcttatgc cacctgcacc
     2461 aatacagtgg acagttacta ttgcgcttgc aaacaaggct tcctgtccag caatgggcaa
     2521 aatcacttca aggatccagg agtgcgatgc aaagatattg atgaatgttc tcaaagcccc
     2581 cagccctgtg gtcctaactc atcctgcaaa aacctgtcag ggaggtacaa gtgcagctgt
     2641 ttagatggtt tctcttctcc cactggaaat gactgggtcc caggaaagcc gggcaatttc
     2701 tcctgtactg atatcaatga gtgcctcacc agcagcgtct gccctgagca ttctgactgt
     2761 gtcaactcca tgggaagcta cagttgcagc tgtcaagttg gattcatctc tagaaactcc
     2821 acctgtgaag acgtggatga atgtgcagat ccaagagctt gcccagagca tgcaacttgt
     2881 aataacactg ttggaaacta ctcttgtttc tgcaacccag gatttgaatc cagcagtggc
     2941 cacttgagtt tccagggtct caaagcatcg tgtgaagata ttgatgaatg cactgaaatg
     3001 tgccccatca attcaacatg caccaacact cctgggagct acttttgcac ctgccaccct
     3061 ggctttgcac caagcaatgg acagttgaat ttcacagacc aaggagtgga atgtagagat
     3121 attgatgagt gccgccaaga tccatcaacc tgtggtccta attctatctg caccaatgcc
     3181 ctgggctcct acagctgtgg ctgcattgca ggctttcatc ccaatccaga aggctcccag
     3241 aaagatggca acttcagctg ccaaagggtt ctcttcaaat gtaaggaaga tgtgataccc
     3301 gataataagc agatccagca atgccaagag ggaaccgcag tgaaacctgc atatgtctcc
     3361 ttttgtgcac aaataaataa catcttcagc gttctggaca aagtgtgtga aaataaaacg
     3421 accgtagttt ctctgaagaa tacaactgag agctttgtcc ctgtgcttaa acaaatatcc
     3481 acgtggacta aattcaccaa ggaagagacg tcctccctgg ccacagtctt cctggagagt
     3541 gtggaaagca tgacactggc atctttttgg aaaccctcag caaatatcac tccggctgtt
     3601 cggacggaat acttagacat tgagagcaaa gttatcaaca aagaatgcag tgaagagaat
     3661 gtgacgttgg acttggtagc caagggggat aagatgaaga tcgggtgttc cacaattgag
     3721 gaatctgaat ccacagagac cactggtgtg gcttttgtct cctttgtggg catggaatcg
     3781 gttttaaatg agcgcttctt caaagaccac caggctccct tgaccacctc tgagatcaag
     3841 ctgaagatga attctcgagt cgttgggggc ataatgactg gagagaagaa agacggcttc
     3901 tcagatccaa tcatctacac tctggagaac attcagccaa agcagaagtt tgagaggccc
     3961 atctgtgttt cctggagcac tgatgtgaag ggtggaagat ggacatcctt tggctgtgtg
     4021 atcctggaag cttctgagac atataccatc tgcagctgta atcagatggc aaatcttgcc
     4081 gttatcatgg cgtctgggga gctcacgatg gacttttcct tgtacatcat tagccatgta
     4141 ggcattatca tctccttggt gtgcctcgtc ttggccatcg ccacctttct gctgtgtcgc
     4201 tccatccgaa atcacaacac ctacctccac ctgcacctct gcgtgtgtct cctcttggcg
     4261 aagactctct tcctcgccgg tatacacaag actgacaaca agatgggctg cgccatcatc
     4321 gcgggcttcc tgcactacct tttccttgcc tgcttcttct ggatgctggt ggaggctgtg
     4381 atactgttct tgatggtcag aaacctgaag gtggtgaatt acttcagctc tcgcaacatc
     4441 aagatgctgc acatctgtgc ctttggttat gggctgccga tgctggtggt ggtgatctct
     4501 gccagtgtgc agccacaggg ctatggaatg cataatcgct gctggctgaa tacagagaca
     4561 gggttcatct ggagtttctt ggggccagtt tgcacagtta tagtgatcaa ctcccttctc
     4621 ctgacctgga ccttgtggat cctgaggcag aggctttcca gtgttaatgc cgaagtctca
     4681 acgctaaaag acaccaggtt actgaccttc aaggcctttg cccagctctt catcctgggc
     4741 tgctcctggg tgctgggcat ttttcagatt ggacctgtgg caggtgtcat ggcttacctg
     4801 ttcaccatca tcaacagcct gcagggggcc ttcatcttcc tcatccactg tctgctcaac
     4861 ggccaggtac gagaagaata caagaggtgg atcactggga agacgaagcc cagctcccag
     4921 tcccagacct caaggatctt gctgtcctcc atgccatccg cttccaagac gggtggatcc
     4981 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     5041 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     5101 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     5161 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5221 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5281 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5341 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5401 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5461 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5521 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5581 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     5641 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     5701 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5761 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5821 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5881 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5941 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     6001 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     6061 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     6121 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     6181 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     6241 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     6301 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     6361 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6421 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6481 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6541 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6601 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6661 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6721 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6781 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6841 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6901 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6961 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7021 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7081 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7141 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7201 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7261 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7321 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7381 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7441 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7501 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7561 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     7621 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7681 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     7741 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7801 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7861 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7921 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7981 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8041 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8101 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     8161 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8221 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8281 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
     8341 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8401 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8461 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8521 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8581 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8641 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8701 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8761 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8821 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8881 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8941 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9001 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9061 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9121 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9181 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9241 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9301 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9361 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9421 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9481 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9541 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9601 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9661 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9721 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9781 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9841 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9901 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9961 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10021 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10081 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10141 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10201 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10261 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10321 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10381 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10441 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10501 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10561 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10621 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10681 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10741 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10801 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10861 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10921 ttgtaaaacg acggccagtg ccaagctg
//
