LOCUS       V029467                11596 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029467
VERSION     V029467
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5622
                     /label="ADNP(NM_015339)"
                     /note="ADNP(NM_015339)"
                     /gene="ADNP"
     CDS             5629..5694
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5738..5949
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5962..6230
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6259..6268
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6289..6951
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7021..7074
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7075..7674
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7675..8263
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8337..8570
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8642..8763
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8803..8938
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8971..8987)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8995..9011
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9019..9049)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9064..9085
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9373..9961)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10132..10992)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10993..11097)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11571..11587
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tccaacttcc tgtcaacaat
     2341 cttggcagtt taagaaaagc ccggaaaact gtgaaaaaaa tacttagtga cattgggttg
     2401 gaatactgta aagaacatat agaagatttt aaacaatttg aacctaatga cttttatttg
     2461 aaaaacacta catgggagga tgtaggactg tgggacccat cacttacgaa aaaccaggac
     2521 tatcggacaa aacctttctg ctgcagcgct tgtccatttt cctcaaaatt cttctctgcc
     2581 tacaaaagtc atttccgcaa tgtccatagt gaagactttg aaaataggat tctccttaat
     2641 tgcccctact gtaccttcaa tgcagacaaa aagactttgg aaacacacat taaaatattt
     2701 catgctccga acgccagcgc accaagtagc agcctcagca ctttcaaaga taaaaacaaa
     2761 aatgatggcc ttaaacctaa gcaggctgac agtgtagagc aagctgttta ttactgtaag
     2821 aagtgcactt accgagatcc tctttatgaa atagttagga agcacattta cagggaacat
     2881 tttcagcatg tggcagcacc ttacatagca aaggcaggag aaaaatcact caatggggca
     2941 gtccccttag gctcgaatgc ccgagaagag agtagtattc actgcaagcg atgccttttc
     3001 atgccaaagt cctatgaagc tttggtacag catgtcatcg aagaccatga acgtataggc
     3061 tatcaggtca ctgccatgat tgggcacaca aatgtagtgg ttccccgatc caaacccttg
     3121 atgctaattg ctcccaaacc tcaagacaag aagagcatgg gactcccacc aaggatcggt
     3181 tcccttgctt ctggaaatgt ccggtcttta ccatcacagc agatggtgaa tcgactctca
     3241 ataccaaagc ctaacttaaa ttctacagga gtcaacatga tgtccagtgt tcatctgcag
     3301 cagaacaact atggagtcaa atctgtaggc cagggttaca gtgttggtca gtcaatgaga
     3361 ctgggtctag gtggcaacgc accagtttcc attcctcaac aatctcagtc tgtaaagcag
     3421 ttacttccaa gtggaaacgg aaggtcttat gggcttgggt cagagcagag gtcccaggca
     3481 ccagcaagat actccctgca gtctgctaat gcctcttctc tctcatcggg ccagttaaag
     3541 tctccttccc tctctcagtc acaggcatcc agagtgttag gtcagtccag ttccaaacct
     3601 gctgcagctg ccacaggccc tcccccaggt aacacttcct caactcaaaa gtggaaaata
     3661 tgtacaatct gtaatgagct ttttcctgaa aatgtctata gtgtgcactt cgaaaaagaa
     3721 cataaagctg agaaagtccc agcagtagcc aactacatta tgaaaataca caattttact
     3781 agcaaatgcc tctactgtaa tcgctattta cccacagata ctctgctcaa ccatatgtta
     3841 attcatggtc tgtcttgtcc atattgccgt tcaactttca atgatgtgga aaagatggcc
     3901 gcacacatgc ggatggttca cattgatgaa gagatgggac ctaaaacaga ttctactttg
     3961 agttttgatt tgacattgca gcagggtagt cacactaaca tccatctcct ggtaactaca
     4021 tacaatctga gggatgcccc agctgaatct gttgcttacc atgcccaaaa taatcctcca
     4081 gttcctccaa agccacagcc aaaggttcag gaaaaggcag atatccctgt aaaaagttca
     4141 cctcaagctg cagtgcccta taaaaaagat gttgggaaaa ccctttgtcc tctttgcttt
     4201 tcaatcctaa aaggacccat atctgatgca cttgcacatc acttacgaga gaggcaccaa
     4261 gttattcaga cggttcatcc agttgagaaa aagctcacct acaaatgtat ccattgcctt
     4321 ggtgtgtata ccagcaacat gaccgcctca actatcactc tgcatctagt tcactgcagg
     4381 ggcgttggaa agacccaaaa tggccaggat aagacaaatg caccctctcg gcttaatcag
     4441 tctccaagtc tggcacctgt gaagcgcact tacgagcaaa tggaatttcc cttactgaaa
     4501 aaacgaaagt tagatgatga tagtgattca cccagcttct ttgaagagaa gcctgaagag
     4561 cctgttgttt tagctttaga ccccaagggt catgaagatg attcctatga agccaggaaa
     4621 agctttctaa caaagtattt caacaaacag ccctatccca ccaggagaga aattgagaag
     4681 ctagcagcca gtttatggtt atggaagagt gacatcgctt cccattttag taacaaaagg
     4741 aagaagtgtg tccgtgattg tgaaaagtac aagcctggcg tgttgctggg gtttaacatg
     4801 aaagaattaa ataaagtcaa gcatgagatg gattttgatg ctgagtggct atttgaaaat
     4861 catgatgaga aggattccag agtcaatgct agtaagactg ctgacaaaaa gctcaacctt
     4921 gggaaggaag atgacagttc ctcagacagt tttgaaaatt tggaagaaga atccaatgaa
     4981 agtggtagcc cttttgaccc tgtttttgaa gttgaaccta aaatctctaa cgataaccca
     5041 gaggaacatg tactgaaggt aattcctgag gatgcttcag aatctgagga gaagctagac
     5101 caaaaagagg atggttcaaa atacgaaact attcatttga ctgaggaacc aaccaaacta
     5161 atgcacaatg catctgatag tgaggttgac caagacgatg ttgttgagtg gaaagacggt
     5221 gcttctccat ctgagagtgg gcctggatcc caacaagtgt cagactttga ggacaatacc
     5281 tgcgaaatga aaccaggaac ctggtctgac gagtcttccc aaagcgaaga tgcaaggagc
     5341 agtaagccag ctgccaaaaa aaaggctacc atgcaaggtg acagagagca gttgaaatgg
     5401 aagaatagtt cctatggaaa agttgaaggg ttttggtcta aggaccagtc acagtggaag
     5461 aatgcatctg agaatgatga gcgcttatct aacccccaga ttgagtggca gaatagcaca
     5521 attgacagtg aggatgggga acagtttgac aacatgactg atggagtagc tgagcccatg
     5581 catggcagct tagccggagt taaactgagc agccaacagg ccggatccga ctacaaagac
     5641 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     5701 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5761 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     5821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5941 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     6001 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     6061 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     6121 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     6181 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     6241 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     6301 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     6361 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     6421 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     6481 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     6541 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     6601 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     6661 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     6721 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     6781 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6841 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6901 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6961 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     7021 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     7081 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7141 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7201 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7261 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7321 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7381 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7441 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7501 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7561 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7621 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     7681 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7741 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7801 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7861 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7921 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7981 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8041 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8101 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8161 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8221 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     8281 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8341 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     8401 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8461 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8521 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8581 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8641 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8761 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8881 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8941 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     9001 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9061 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9121 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9181 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9781 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10021 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10081 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10141 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10201 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10261 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10321 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10381 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10441 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10501 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10561 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10621 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10681 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10741 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10801 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10861 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10921 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10981 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11041 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11101 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    11161 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    11221 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    11281 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    11341 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    11401 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    11461 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    11521 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    11581 ggccagtgcc aagctg
//
