LOCUS       V038925                10921 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038925
VERSION     V038925
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             3703..3756
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             3757..5841
                     /label="APP(NM_201414)"
                     /note="APP(NM_201414)"
                     /gene="APP"
     promoter        5891..6102
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6115..6383
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6410..7009
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7016..7604
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7678..7911
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7983..8104
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8123..8258
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8296..8312)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8320..8336
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8344..8374)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8389..8410
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8698..9286)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9457..10317)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10318..10422)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10896..10912
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgacagag ttacctgcac cgttgtccta cttccagaat
     2341 gcacagatgt ctgaggacaa ccacctgagc aatactgtac gtagccagaa tgacaataga
     2401 gaacggcagg agcacaacga cagacggagc cttggccacc ctgagccatt atctaatgga
     2461 cgaccccagg gtaactcccg gcaggtggtg gagcaagatg aggaagaaga tgaggagctg
     2521 acattgaaat atggcgccaa gcatgtgatc atgctctttg tccctgtgac tctctgcatg
     2581 gtggtggtcg tggctaccat taagtcagtc agcttttata cccggaagga tgggcagcta
     2641 atctataccc cattcacaga agataccgag actgtgggcc agagagccct gcactcaatt
     2701 ctgaatgctg ccatcatgat cagtgtcatt gttgtcatga ctatcctcct ggtggttctg
     2761 tataaataca ggtgctataa ggtcatccat gcctggctta ttatatcatc tctattgttg
     2821 ctgttctttt tttcattcat ttacttgggg gaagtgttta aaacctataa cgttgctgtg
     2881 gactacatta ctgttgcact cctgatctgg aattttggtg tggtgggaat gatttccatt
     2941 cactggaaag gtccacttcg actccagcag gcatatctca ttatgattag tgccctcatg
     3001 gccctggtgt ttatcaagta cctccctgaa tggactgcgt ggctcatctt ggctgtgatt
     3061 tcagtatatg atttagtggc tgttttgtgt ccgaaaggtc cacttcgtat gctggttgaa
     3121 acagctcagg agagaaatga aacgcttttt ccagctctca tttactcctc aacaatggtg
     3181 tggttggtga atatggcaga aggagacccg gaagctcaaa ggagagtatc caaaaattcc
     3241 aagtataatg cagaaagcac agaaagggag tcacaagaca ctgttgcaga gaatgatgat
     3301 ggcgggttca gtgaggaatg ggaagcccag agggacagtc atctagggcc tcatcgctct
     3361 acacctgagt cacgagctgc tgtccaggaa ctttccagca gtatcctcgc tggtgaagac
     3421 ccagaggaaa ggggagtaaa acttggattg ggagatttca ttttctacag tgttctggtt
     3481 ggtaaagcct cagcaacagc cagtggagac tggaacacaa ccatagcctg tttcgtagcc
     3541 atattaattg gtttgtgcct tacattatta ctccttgcca ttttcaagaa agcattgcca
     3601 gctcttccaa tctccatcac ctttgggctt gttttctact ttgccacaga ttatcttgta
     3661 cagcctttta tggaccaatt agcattccat caattttata tcgagggcag aggaagtctt
     3721 ctaacatgcg gtgacgtgga ggagaatccc ggccctatgc tgcccggttt ggcactgctc
     3781 ctgctggccg cctggacggc tcgggcgctg gaggtaccca ctgatggtaa tgctggcctg
     3841 ctggctgaac cccagattgc catgttctgt ggcagactga acatgcacat gaatgtccag
     3901 aatgggaagt gggattcaga tccatcaggg accaaaacct gcattgatac caaggaaggc
     3961 atcctgcagt attgccaaga agtctaccct gaactgcaga tcaccaatgt ggtagaagcc
     4021 aaccaaccag tgaccatcca gaactggtgc aagcggggcc gcaagcagtg caagacccat
     4081 ccccactttg tgattcccta ccgctgctta gttggtgagt ttgtaagtga tgcccttctc
     4141 gttcctgaca agtgcaaatt cttacaccag gagaggatgg atgtttgcga aactcatctt
     4201 cactggcaca ccgtcgccaa agagacatgc agtgagaaga gtaccaactt gcatgactac
     4261 ggcatgttgc tgccctgcgg aattgacaag ttccgagggg tagagtttgt gtgttgccca
     4321 ctggctgaag aaagtgacaa tgtggattct gctgatgcgg aggaggatga ctcggatgtc
     4381 tggtggggcg gagcagacac agactatgca gatgggagtg aagacaaagt agtagaagta
     4441 gcagaggagg aagaagtggc tgaggtggaa gaagaagaag ccgatgatga cgaggacgat
     4501 gaggatggtg atgaggtaga ggaagaggct gaggaaccct acgaagaagc cacagagaga
     4561 accaccagca ttgccaccac caccaccacc accacagagt ctgtggaaga ggtggttcga
     4621 gttcctacaa cagcagccag tacccctgat gccgttgaca agtatctcga gacacctggg
     4681 gatgagaatg aacatgccca tttccagaaa gccaaagaga ggcttgaggc caagcaccga
     4741 gagagaatgt cccaggtcat gagagaatgg gaagaggcag aacgtcaagc aaagaacttg
     4801 cctaaagctg ataagaaggc agttatccag catttccagg agaaagtgga atctttggaa
     4861 caggaagcag ccaacgagag acagcagctg gtggagacac acatggccag agtggaagcc
     4921 atgctcaatg accgccgccg cctggccctg gagaactaca tcaccgctct gcaggctgtt
     4981 cctcctcggc ctcgtcacgt gttcaatatg ctaaagaagt atgtccgcgc agaacagaag
     5041 gacagacagc acaccctaaa gcatttcgag catgtgcgca tggtggatcc caagaaagcc
     5101 gctcagatcc ggtcccaggt tatgacacac ctccgtgtga tttatgagcg catgaatcag
     5161 tctctctccc tgctctacaa cgtgcctgca gtggccgagg agattcagga tgaagttgat
     5221 gagctgcttc agaaagagca aaactattca gatgacgtct tggccaacat gattagtgaa
     5281 ccaaggatca gttacggaaa cgatgctctc atgccatctt tgaccgaaac gaaaaccacc
     5341 gtggagctcc ttcccgtgaa tggagagttc agcctggacg atctccagcc gtggcattct
     5401 tttggggctg actctgtgcc agccaacaca gaaaacgaag ttgagcctgt tgatgcccgc
     5461 cctgctgccg accgaggact gaccactcga ccaggttctg ggttgacaaa tatcaagacg
     5521 gaggagatct ctgaagtgaa gatggatgca gaattccgac atgactcagg atatgaagtt
     5581 catcatcaaa aattggtgtt ctttgcagaa gatgtgggtt caaacaaagg tgcaatcatt
     5641 ggactcatgg tgggcggtgt tgtcatagcg acagtgatcg tcatcacctt ggtgatgctg
     5701 aagaagaaac agtacacatc cattcatcat ggtgtggtgg aggttgacgc cgctgtcacc
     5761 ccagaggagc gccacctgtc caagatgcag cagaacggct acgaaaatcc aacctacaag
     5821 ttctttgagc agatgcagaa ctaggatccg cggccgcgaa ggatctgcga tcgctccggt
     5881 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5941 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6001 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6061 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     6121 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     6181 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     6241 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6301 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6361 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta
     6421 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     6481 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     6541 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6601 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6661 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6721 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6781 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6841 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6901 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6961 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca
     7021 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7081 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7141 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7201 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7261 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7321 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7381 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7441 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7501 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7561 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     7621 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7681 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     7741 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7801 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7861 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7921 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7981 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8041 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8101 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     8161 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8221 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     8281 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8341 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8401 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8461 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9121 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10441 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10501 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10561 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10621 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10681 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10741 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10801 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10861 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    10921 g
//
