LOCUS       V036493                15034 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036493
VERSION     V036493
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..9060
                     /label="ASXL3(NM_030632)"
                     /note="ASXL3(NM_030632)"
                     /gene="ASXL3"
     CDS             9067..9132
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9176..9387
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9400..9668
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      9697..9706
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             9727..10389
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             10459..10512
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             10513..11112
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11113..11701
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11775..12008
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12080..12201
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12241..12376
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12409..12425)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12433..12449
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12457..12487)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12502..12523
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12811..13399)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13570..14430)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14431..14535)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15009..15025
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga aagacaagag gaagaagaag
     2341 gaccgcacct gggccgaggc tgcccgcctg gcactagaaa aacaccccaa ctcaccaatg
     2401 acagcaaagc agatattgga agtcattcag aaagaagggt taaaagaaac aagtggaacc
     2461 tctccattag cctgtctgaa tgcaatgctt cacactaaca ctcgaatagg ggatggaaca
     2521 ttcttcaaaa tccctggaaa gtcaggcctc tatgctctca aaaaagagga gtcgtcatgc
     2581 ccagcagatg gcacgttgga tttagtctgt gaatctgaat tggatggtac agatatggcc
     2641 gaggcaaatg cccatggaga agaaaatgga gtttgttcga agcaggtaac tgatgaagca
     2701 tcttccactc gagattcaag ccttactaac acagcagtgc aaagcaagtt agtgtcttcc
     2761 ttccagcagc acaccaaaaa ggctcttaaa caggctttga ggcagcagca gaaaagaaga
     2821 aatggagtct caatgatggt aaacaagact gttcctcgtg ttgttttgac accattaaag
     2881 gtgtctgatg agcagtcgga ttcgccttca ggatctgaat ctaaaaatgg tgaagcagac
     2941 agttcagata aagaaatgaa acatgggcaa aaatctccca ctggaaaaca aacaagtcag
     3001 cacttaaaac gattaaaaaa gtctggttta gggcacttga aatggaccaa agctgaggac
     3061 attgacatag aaaccccagg atctattctt gtcaacacta acttgagggc attaataaat
     3121 aaacatacgt ttgcttcctt acctcagcat tttcaacaat acctcctgct tttgctccca
     3181 gaagtggata ggcagatggg aagtgatgga attttacgcc tcagtacttc agctctaaat
     3241 aatgaattct ttgcatatgc agcacaaggg tggaaacagc gactggcaga aggagagttt
     3301 accccagaaa tgcagttgcg gataaggcaa gaaattgaga aggaaaagaa aacagaacct
     3361 tggaaagaaa aattctttga gaggttttat ggagaaaagc tgggcatgtc aagagaggaa
     3421 tctgtgaagc tcactactgg accaaacaac gctggagctc aaagtagttc ttcatgtggg
     3481 acttctggcc ttccagtttc tgcacagaca gccttggcag aacaacagcc aaaaagcatg
     3541 aaaagcccag cttctccaga gcctggtttc tgtgctactc tttgccctat ggtagaaatt
     3601 ccacctaaag atataatggc agaattggag tcagaggata tcttgatccc tgaagaatct
     3661 gtaattcagg aggaaattgc agaagaggta gagactagta tctgtgaatg ccaggatgaa
     3721 aatcataaga caatacctga attttctgag gaggctgaaa gtctaaccaa ttctcatgaa
     3781 gaaccccaaa tagcacctcc tgaagataac ttggaatcct gtgttatgat gaatgatgtt
     3841 ttagaaactt tgcctcatat tgaagttaag atagaaggga agtcagaatc accccaggaa
     3901 gaaatgacag ttgttatcga tcagttagaa gtctgtgact ctcttattcc ttccacttca
     3961 tctatgactc atgtcagtga cacagaacat aaggagtcag aaactgcagt agagaccagt
     4021 acccccaaaa taaaaacagg gtcatcttct ctagaaggcc agtttccaaa tgaaggaatt
     4081 gctatagata tggagctaca gagtgaccct gaagaacagc tttcagaaaa tgcctgcatc
     4141 tctgaaacgt ccttttcttc tgagagccca gagggagcct gtaccagcct gccttctcca
     4201 ggaggggaaa cacagtccac atcagaagaa tcatgtactc cagcctccct tgagacaaca
     4261 ttttgttctg aggtatctag cactgaaaat acagacaaat acaaccagag aaattccact
     4321 gatgaaaact ttcatgcatc tttgatgtca gaaatatctc caatatccac ttcacctgaa
     4381 atatcagaag catctcttat gtccaactta ccattaacat ctgaagcatc accagtatcc
     4441 aacttacctt taacatcaga aacctcaccg atgtctgact tacctttaac atcagaaact
     4501 tcttcagtgt cttccatgct tctcacctct gagaccactt ttgtatccag tttgccactt
     4561 ccttcagaaa catctccaat ttccaactct tccataaatg agagaatggc acatcagcaa
     4621 agaaagtcac cttctgtatc tgaagagcca ctctccccgc agaaagatga gtcttccgcc
     4681 actgccaaac ctctgggaga gaaccttacc tcccagcaga agaatctgtc taatactccc
     4741 gaacccatca taatgagttc ttcttccatt gctcctgaag catttccgtc tgaagatttg
     4801 cacaataaga ccctgagtca gcaaacctgt aaatcacatg ttgacactga gaagccctac
     4861 cctgcttcaa ttccagaact tgcttctact gaaatgataa aagttaaaaa tcatagcgtc
     4921 ctgcaaagaa cagaaaaaaa agtgttacct tcaccattgg aattatctgt cttttctgaa
     4981 gggacagata ataagggaaa tgagcttcca tctgctaaat tacaggacaa gcaatatatc
     5041 tcatcagtgg ataaggctcc attttcagaa ggctctagaa ataaaacaca taagcaaggg
     5101 agtacacaga gtcggttaga aacctcacat acttccaagt catcagagcc ctccaagtca
     5161 cctgatggga taagaaatga aagtagagat tcagagatat caaagagaaa aactgcagag
     5221 caacacagct ttggaatctg taaggaaaag agagctagga tagaagatga tcagtcaacc
     5281 cggaacatat catctagcag cccacctgag aaagaacagc ctcccagaga ggaaccaagg
     5341 gttccccctc tcaagattca gctttccaaa attgggccac cttttataat caagagccaa
     5401 ccagtctcca aacctgagtc tcgagcatcc actagcacat ctgtcagtgg cgggaggaac
     5461 acaggagcca ggaccctcgc agatatcaag gcccgggccc aacaagctcg ggcccagcga
     5521 gaggctgctg cagctgctgc tgtggctgct gcagcgagca ttgtctctgg agccatggga
     5581 agtccaggag agggtggaaa gacgagaact ctggcacaca tcaaagagca gacaaaggct
     5641 aagctctttg caaagcatca agctcgagcc catctcttcc agacctctaa agagacccgg
     5701 ttgcctcctc cgctcagctc aaaggaaggg cctccaaact tagaagtctc ttctacccct
     5761 gaaacaaaaa tggaaggttc gactggtgtc attattgtca atccaaactg tagatctcct
     5821 agcaacaagt ctgcccacct ccgggagacc accactgtac tacagcagtc tcttaaccca
     5881 agtaaacttc cagaaactgc cactgactta tctgtgcata gttctgatga aaacatacct
     5941 gtgtcacatt tatctgagaa aattgtttca tctacctctt ctgaaaatag cagtgtgccc
     6001 atgcttttta ataaaaattc tgtccctgta tctgtttgca gcactgctat atcgggagca
     6061 attaaagaac atccctttgt gagttctgtt gataaatcct ctgtcctaat gtctgttgac
     6121 agtgcaaaca ctacaatttc tgcttgtaat ataagcatgt taaaaaccat ccagggaact
     6181 gacactccat gcatagccat tataccaaaa tgtattgaaa gcactcccat ttcagccact
     6241 acagagggct ccagcatatc aagctccatg gatgataagc agttactaat atcaagcagc
     6301 agtgctagta acttagtctc cactcagtac acctctgtgc caactccctc catcggaaac
     6361 aatttgccaa acctctccac tagctctgtc ttgattcccc caatgggaat taacaacaga
     6421 tttccttctg agaagatagc catacctggg agtgaagaac aggccactgt atccatgggt
     6481 accactgtga gagcagccct cagctgcagt gattctgtag cggtcacaga ctctctggtt
     6541 gcacacccga ccgtcgcaat gtttactgga aacatgctga caataaactc ttatgatagt
     6601 cctcccaagt taagtgctga aagcttggac aaaaattcag ggcctcgaaa cagggcagat
     6661 aattctggaa aacctcagca accaccaggg ggctttgcac cagcagccat aaaccgatca
     6721 attccgtgta aagtcatcgt tgaccacagc accacgctga cctccagttt gtctctgact
     6781 gtctccgttg aaagctcaga agccagcttg gacctgcagg gcagaccagt gaggacagag
     6841 gcatccgtac agcccgtggc gtgtcctcag gtgtctgtga ttagcaggcc tgagccagtt
     6901 gccaacgaag gtatagatca cagttccact ttcattgctg cttcggcagc aaaacaagac
     6961 agtaaaacat tgccggccac ctgcacaagt ctccgagaat taccccttgt tccagataaa
     7021 ttaaatgagc cgactgctcc cagtcataac tttgctgagc aggcacgtgg cccagctcct
     7081 ttcaaaagtg aagcagacac aacctgtagc aatcagtata acccaagtaa ccggatttgc
     7141 tggaatgatg atgggatgag gagcacagga cagcctctgg ttactcactc gggttcaagt
     7201 aaacaaaaag aatatctaga gcaaagctgt ccaaaggcta tcaaaactga acatgccaac
     7261 tacttgaacg tgtcagaact tcatcccagg aatcttgtaa caaatgttgc tcttcctgtg
     7321 aaatctgaac ttcacgaagc agacaagggc tttagaatgg acactgaaga cttccctggc
     7381 cctgagctgc ctcctccggc tgcagaggga gcctctagtg tacaacaaac acagaacatg
     7441 aaagcttcca cctcaagtcc catggaagag gctatttcct tggctaccga tgccctgaag
     7501 agagtccctg gtgcagggag ctcaggctgt cgtctgtcct ctgtggaggc taacaatccg
     7561 ctggtgacgc agttactaca gggcaacctg cctttggaaa aagtgttgcc acagcccaga
     7621 ttgggagcca agcttgaaat caacaggctt ccattgcctc ttcaaactac ctcagtgggt
     7681 aaaacagcac cagagagaaa cgttgaaatt ccgcccagct ctccaaatcc agatggtaag
     7741 ggctacttgg cagggactct ggcaccactc caaatgagaa agcgagaaaa ccaccccaaa
     7801 aagagagtag ctaggactgt aggagaacac actcaagtta aatgtgaacc aggaaaattg
     7861 ttggtggagc cagatgttaa aggggtgcct tgtgtcatca gttccggcat cagtcagcta
     7921 ggacacagcc agccatttaa gcaagaatgg ctaaacaagc actccatgca gaacagaatt
     7981 gttcacagcc ctgaggtcaa acagcaaaag cggctgctcc cctcgtgtag cttccagcag
     8041 aacctatttc atgttgacaa gaatggcggc ttccacactg acgctggtac ctcacacaga
     8101 cagcagtttt accaaatgcc tgtggctgcc aggggcccca ttcctactgc agctctgtta
     8161 caggcctctt ccaagacccc agtggggtgt aatgcatttg ccttcaacag gcatcttgaa
     8221 cagaagggat tgggagaggt tagtctttcc tcagcacctc accagctaag gttagccaac
     8281 atgttatccc ccaatatgcc catgaaagaa ggtgatgagg tgggaggcac tgcacacaca
     8341 atgccaaaca aagcactagt acatccgccg ccgccaccgc ctccccctcc ccctccaccc
     8401 ttggctttgc ccccgcctcc ccccccacca cctccgctac ctccacctct ccctaatgca
     8461 gaagtcccat ctgatcaaaa acaacctcca gttaccatgg aaaccactaa gagacttagt
     8521 tggccacagt ccacgggcat atgtagcaat ataaaatcgg aacctctttc ttttgaggaa
     8581 ggtttaagca gcagctgtga actgggcatg aaacaagttt cctatgacca gaatgaaatg
     8641 aaagaacagt taaaagcatt cgcgctaaaa agtgcagatt tctcttccta tttgctttct
     8701 gagccacaaa agccttttac ccaattagct gctcagaaaa tgcaggtgca gcaacaacag
     8761 cagctctgtg gaaattatcc aacaatacac tttggtagca cgagtttcaa aagggcagca
     8821 tctgcaattg aaaagtccat tgggattttg ggaagtggct ccaatcctgc cacaggcttg
     8881 tctggtcaga acgctcagat gcccgttcag aactttgccg acagcagcaa tgcagatgaa
     8941 ttggaactga aatgctcttg ccggctgaaa gccatgattg tgtgcaaagg ctgtggggcc
     9001 ttctgccatg acgactgcat aggtccttca aaactttgtg tagcatgcct ggttgtacga
     9061 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     9121 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     9181 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     9241 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     9301 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     9361 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     9421 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     9481 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     9541 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     9601 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     9661 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     9721 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     9781 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     9841 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     9901 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     9961 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
    10021 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
    10081 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
    10141 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
    10201 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
    10261 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
    10321 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
    10381 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
    10441 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
    10501 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
    10561 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
    10621 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
    10681 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
    10741 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
    10801 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
    10861 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
    10921 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
    10981 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
    11041 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
    11101 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
    11161 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
    11221 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
    11281 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
    11341 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
    11401 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    11461 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    11521 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    11581 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    11641 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    11701 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
    11761 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
    11821 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    11881 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    11941 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    12001 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
    12061 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
    12121 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    12181 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
    12241 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    12301 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    12361 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
    12421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    12481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    12541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    12601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    12661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    12721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    12781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    12841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    12901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    12961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    13021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    13081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    13141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    13201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    13261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    13321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    13381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    13441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    13501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    13561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    13621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    13681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    13741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    13801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    13861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    13921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    13981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    14041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    14101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    14161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    14221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    14281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    14341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    14401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    14461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    14521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    14581 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    14641 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    14701 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    14761 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    14821 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    14881 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    14941 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    15001 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
