LOCUS       V044081                10258 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044081
VERSION     V044081
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3261
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID
                     engineered for improved catalytic efficiency (Branon et
                     al., 2018)"
                     /transl_table=1
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="TurboID"
     CDS             3268..3294
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3298..3324
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3328..3354
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3355..5112
                     /label="ATAD3A(NM_001170535)"
                     /note="ATAD3A(NM_001170535)"
                     /gene="ATAD3A"
     CDS             5119..5184
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5228..5439
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5452..5720
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5747..6346
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6353..6941
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7015..7248
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7320..7441
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7460..7595
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7633..7649)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7657..7673
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7681..7711)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7726..7747
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8035..8623)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8794..9654)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9655..9759)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10233..10249
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaaagac aatactgtgc ctctgaagct gatcgctctc
     2341 ctggctaatg gcgagttcca tagtggcgaa cagctgggag aaaccctggg catgtccagg
     2401 gccgctatca acaagcacat tcagactctg cgcgactggg gcgtggacgt gttcaccgtg
     2461 cccggaaagg gctactctct gcccgagcct atcccgctgc tgaacgctaa acagattctg
     2521 ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac
     2581 ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag
     2641 gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg
     2701 agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga
     2761 attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc
     2821 aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata
     2881 acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg
     2941 gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac
     3001 aggaatactc tggccgctac gctgatccga gagctgcggg cagccctgga actgttcgag
     3061 caggaaggcc tggctccata tctgccacgg tgggagaagc tggataactt catcaataga
     3121 cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa
     3181 cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc
     3241 agtctgcggt ctgccgaaaa gctcgagtac ccatacgacg tacctgatta tgcaacttat
     3301 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcaatgtcg
     3361 tggctcttcg gcattaacaa gggccccaag ggtgaaggcg cggggccgcc gccgcctttg
     3421 ccgcccgcgc agcccggggc cgagggcggc ggggaccgcg ggttgggaga ccggccggcg
     3481 cccaaggaca aatggagcaa cttcgacccc accggcctgg agcgcgccgc caaggcggcg
     3541 cgcgagctgg agcactcgcg ttatgccaag gacgccctga atctggcaca gatgcaggag
     3601 cagacgctgc agttggagca acagtccaag ctcaaagagt atgaggccgc cgtggagcag
     3661 ctcaagagcg agcagatccg ggcgcaggct gaggagagga ggaagaccct gagcgaggag
     3721 acccggcagc accaggccag ggcccagtat caagacaagc tggcccggca gcgctacgag
     3781 gaccaactga agcagcagca acttctcaat gaggagaatt tacggaagca ggaggagtcc
     3841 gtgcagaagc aggaagccat gcggcgagcc accgtggagc gggagatgga gctgcggcac
     3901 aagaatgaga tgctgcgagt ggaggccgag gcccgggcgc gcgccaaggc cgagcgggag
     3961 aatgcagaca tcatccgcga gcagatccgc ctgaaggcgg ccgagcaccg tcagaccgtc
     4021 ttggagtcca tcaggacggc tggcaccttg tttggggaag gattccgtgc ctttgtgaca
     4081 gactgggaca aagtgacagc cacggtggct gggctgacgc tgctggctgt tggggtctac
     4141 tcagccaaga atgccacgct tgtcgccggc cgcttcatcg aggctcggct ggggaagccg
     4201 tccctagtga gggagacgtc ccgcatcacg gtgcttgagg cgctgcggca ccccatccag
     4261 gtcagccggc ggctcctcag tcgaccccag gacgcgctgg agggtgttgt gctcagtccc
     4321 agcctggaag cacgggtgcg cgacatcgcc atagcaacaa ggaacaccaa gaagaaccgc
     4381 agcctgtaca ggaacatcct gatgtacggg ccaccaggca ccgggaagac gctgtttgcc
     4441 aagaaactcg ccctgcactc aggcatggac tacgccatca tgacaggcgg ggacgtggcc
     4501 cccatggggc gggaaggcgt gaccgccatg cacaagctct ttgactgggc caataccagc
     4561 cggcgcggcc tcctgctctt tgtggatgaa gcggacgcct tccttcggaa gcgagccacc
     4621 gagaagataa gcgaggacct cagggccaca ctgaacgcct tcctgtaccg cacgggccag
     4681 cacagcaaca agttcatgct ggtcctggcc agcaaccaac cagagcagtt cgactgggcc
     4741 atcaatgacc gcatcaatga gatggtccac ttcgacctgc cagggcagga ggaacgggag
     4801 cgcctggtga gaatgtattt tgacaagtat gttcttaagc cggccacaga aggaaagcag
     4861 cgcctgaagc tggcccagtt tgactacggg aggaagtgct cggaggtcgc tcggctgacg
     4921 gagggcatgt cgggccggga gatcgctcag ctggccgtgt cctggcaggc cacggcgtat
     4981 gcctccgagg acggggtcct gaccgaggcc atgatggaca cccgcgtgca agatgctgtc
     5041 cagcagcacc agcagaagat gtgctggctg aaggcggaag ggcctgggcg tggggacgag
     5101 ccctccccat ccggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     5161 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     5221 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5281 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5341 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5401 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     5461 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     5521 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     5581 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     5641 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     5701 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa
     5761 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     5821 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     5881 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5941 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6001 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6061 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6121 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6181 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6241 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6301 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc
     6361 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6421 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6481 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6541 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6601 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6661 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6721 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6781 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6841 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6901 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
     6961 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7021 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt
     7081 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7141 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7201 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7261 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     7321 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7381 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7441 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc
     7501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7561 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa
     7621 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7681 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7741 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7801 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7861 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7921 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7981 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8041 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8101 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8161 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8221 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8281 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8341 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8401 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8461 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8521 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9781 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     9841 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     9901 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     9961 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    10021 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    10081 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10141 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    10201 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
