LOCUS       V027112                10336 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027112
VERSION     V027112
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4362
                     /label="Acss1(NM_080575)"
                     /note="Acss1(NM_080575)"
                     /gene="Acss1"
     CDS             4369..4434
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4478..4689
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4702..4970
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4999..5008
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5029..5691
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5761..5814
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5815..6414
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6415..7003
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7077..7310
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7382..7503
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7543..7678
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7711..7727)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7735..7751
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7759..7789)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7804..7825
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8113..8701)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8872..9732)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9733..9837)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10311..10327
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcgcgcag cctcggcagc
     2341 ggtgtggggc gactgctgcg tggcctgcaa gggcgctctg gacaatcggg gtggtcactg
     2401 agcgtgtccc gctcgaccgc cacccgactc ccgggctgcg ttcctgcagc agcgcaacca
     2461 ggctcgtacc ccgcgttgag cgctcaggca gcccaggagc cggccgcctt ctgggggccg
     2521 ctggcccgtg acacactagt ttgggacact ccttaccata ctgtctggga ctgtgacttc
     2581 aggacgggca agatcggctg gttcctggga ggccagttga atgtgtctgt caactgcctg
     2641 gatcagcatg tgcagaagtc tcctgagacc atagctttga tctgggagag agatgaacct
     2701 gggacagaag tgagaatcac ttacagggag cttctggaga ccacatgccg cctggccaat
     2761 acactgaaga gacatggagt gcaccgaggg gaccgtgtgg ccatctacat gcctgtgtcc
     2821 ccactggctg tggcagcaat gctggcctgt gccaggattg gagctatcca cacagtggta
     2881 tttgctggct ttagtgcaga gtccttggct gggaggatca atgatgccaa atgcaaggct
     2941 gttatcacct tcaaccaagg actcagggga gggcgtgtgg tggagctgaa gaaaatagtg
     3001 gatgaagctg tgaagagctg cccaacagtc cagcatgtcc tggtggctca caggacggac
     3061 accaaggttc ccatggggag tctggatatc ccccttgaac aggagatggc caaggaggcc
     3121 cctgtttgca cccctgagag catgagcagt gaagacatgc tctttatgct ctacacctca
     3181 gggagcaccg ggacacccaa gggactcgtt catacacagg caggctatct actgtatgcc
     3241 gccatgacgc acaagctcgt gtttgactac cagccaggtg atgtctttgg ctgtgtggct
     3301 gacatcggtt ggatcacagg acacagctat gtggtgtatg gacccctctg caatggagct
     3361 accacagtcc tttttgagag caccccagtt taccctgatg ctggtcgtta ctgggagaca
     3421 gtgcagaggc taaagatcaa ccagttctat ggtgccccga cagctgtccg gctgctgctg
     3481 aagtatgggg atgcctgggt gaaaaagtat gaccgctctt ccctgcgcac actggggtca
     3541 gtgggagagc ctatcaacca cgaagcctgg gagtggctcc acaaagtcgt gggtgatggc
     3601 agatgtacac tggtggacac ttggtggcaa acggaaactg gaggcatctg cattgcacca
     3661 cggccctcgg aagatggggc agagatcctc ccgggcatgg ccatgaggcc gttttttggc
     3721 atcgttcctg tactcatgga tgagaagggc aatgttttgg agggtggaga tgtctctggg
     3781 gccttgtgta tttcccaagc ttggccaggc atggcaagga ccatctatgg tgaccaccag
     3841 aggttcgtag atgcctactt cagagcgtac ccaggttatt acttcactgg agacggagct
     3901 caccggacag agggtggcta ttaccagatc acggggcgca tggatgatgt catcaatatc
     3961 agtggtcatc gcctggggac tgcagagatt gaggatgcaa tggctgacca tcccgctgtt
     4021 ccagagactg ctgtcattgg gtaccctcat gatatcaaag gagaagctgc atttgccttc
     4081 attgtgctga aagataacat cagtgatgag aacatggtag tgaatgaact caaattgtcg
     4141 gtggccacca agatcgccaa gtatgctgtg cctgaccaga tcctggtggt gaagcgtctc
     4201 cccaaaacca gatctgggaa agtgatgagg agactactga ggaagatcat caccagcagg
     4261 ggacaggatc taggggacac cactaccctg gaggacccca gcgtcatcac agaaatcttg
     4321 agtgccttcc agaagtatga agagcagcgg gctgctacca acggatccga ctacaaagac
     4381 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4441 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4501 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4561 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4621 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4681 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     4741 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     4801 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     4861 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     4921 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     4981 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     5041 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5101 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5161 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5221 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5281 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5341 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5401 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5461 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5521 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5581 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     5641 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     5701 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     5761 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5821 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5881 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5941 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6001 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6061 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6121 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6181 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6241 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6301 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6361 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6421 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6481 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6541 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6601 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6661 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6781 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6841 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6901 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6961 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     7021 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7081 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7141 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7201 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7261 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7321 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7381 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7501 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7621 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7681 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     7741 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7801 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7861 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7921 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7981 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8041 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8281 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8341 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8401 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8461 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8521 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8581 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8641 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8701 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8761 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8821 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8881 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8941 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9001 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9061 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9841 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9901 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     9961 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10021 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10081 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10141 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10201 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10261 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10321 ggccagtgcc aagctg
//
