LOCUS       V038604                11308 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038604
VERSION     V038604
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5334
                     /label="Agbl3(NM_001289656)"
                     /note="Agbl3(NM_001289656)"
                     /gene="Agbl3"
     CDS             5341..5406
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5450..5661
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5674..5942
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5971..5980
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6001..6663
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6733..6786
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6787..7386
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7387..7975
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8049..8282
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8354..8475
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8515..8650
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8683..8699)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8707..8723
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8731..8761)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8776..8797
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9085..9673)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9844..10704)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10705..10809)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11283..11299
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cagaagattc agaggaggaa
     2341 gactactcag atagaagcat cagtgatgac gatgacttgg atgaggattc cttcatgaaa
     2401 tttgtaagtg atgatattca tccgtgcaca ctactggcag ctgattctat tggtgaccca
     2461 ttcttccctc ggactacaca aattttattg gagtatcagc taggaagatg ggtaccacgc
     2521 cttcgcggac cacgagattt atatggtgtc tcttcttctg gtccactgag cccaacacgg
     2581 tggccatacc actgtgaggt cattgatgaa aaagtccagc atattgaatg gactcccttt
     2641 gtccctgagc cagtgtatgt cccgacaggc ctggaaatag agcctgttta cccaaactcc
     2701 aaggaagata ctgtggttta tttagctgaa gatgatcacc tttgtaaagc ttacaaagag
     2761 ccctgttttg tgtattcccg agttgggggt aaccgaacat ccttgaaaca acctgtggat
     2821 aactgtgaca acacgctggt gtttgaagca aggtttgaga gtggtaatct acagaaggta
     2881 gtcaaagttg cagatcatga atacgagttg actgtgcgcc cagacctctt cacaaataag
     2941 cacactcagt ggtactattt ccaggtcact aatacccaag cagaaatagt ctacagattc
     3001 actattgtca actttaccaa gcctgctagt ctttacaatc ggggtatgaa gccattgttc
     3061 tattctgaaa aagaggccaa aacccataac attggctggc agagaatagg agatcaaatc
     3121 aagtattata aaaataacct tgggcaagat gggcgccatt ttttttctct tacgtggaca
     3181 tttcagtttc cacacagcca ggatacgtgc tactttgctc actgctatcc atacacttac
     3241 tccaaccttc aagaatacct gtctggcatc aacagtgacc cagtaagatc aaagttttgt
     3301 aaaatacgtg tcttgtgtca cacacttgcc aggaatatgg tgtatgtctt gacaataact
     3361 actcccttga agactagtga ctcaaagcgc aaggctgtga ttttgactgc aagggtccat
     3421 ccaggagaga ccaacagctc ttggatcatg aaaggcttcc tggattatat tttaggagac
     3481 tcaagtgatg cacgcttgct tcgggacact ttcatcttca aggtggtacc catgctgaat
     3541 ccagatggcg tgattgtggg aaattatcga tgttccttag ctggacggga tttaaaccgt
     3601 aattacacat cgctcctgaa ggagtctttc ccttctgttt ggtatacaag gaacatgatc
     3661 aataggttaa tggagaaacg agaggttatt ttatattgtg atcttcatgg ccatagtagg
     3721 aaacagaata ttttcatgta tggttgtgat ggtagcagca gatctaaaac aaaaggatta
     3781 tacttacagc aacggatctt tccactcatg ctgagcaaaa attgcccaaa tatattttcc
     3841 ttctcagctt gcaagtttaa cgttcagaaa agcaaagagg ggacaggaag ggttgtaatg
     3901 tggaagatgg gcatcaggaa cagcttcacg ctggaggcca ccttctgcgg atctactctg
     3961 ggcaataaac gaggcactca tttcggcaca aaagatttag agtcgatggg atatcatttt
     4021 tgtgattctc tgttggacta ctgtgatcct gacaggagca aatattatca gtgcttgaaa
     4081 gaattagaag aaatggagaa acatttgagc tccgaaagag tctctgataa cacagatact
     4141 tctctagtag aaatttcttt ggatgtagag tctagtagtc gaggctctga cagctcagag
     4201 tccaatgaca ctcagactta ccttctcaag gtgacctctc aggcaagaaa caagaaaaag
     4261 tatctgaaaa caaagaggga aagaaatgcc atcctagcaa actgccagaa taacatgcaa
     4321 gaggtttatg gcaaagaaca tttactccaa agacacgatg aatcaaattc tgatgggaat
     4381 gatccaagga tagatgcacc agatgtttat gtggctcact gtttcagaag accgttgcct
     4441 aaccaaggcg tggtgaaaat tcctggacaa aggttttatc ccggaaaaac atggtccagc
     4501 tcccagcgta tgattaagag tcttaacaaa gaccatagga catgtatttt agaaacttgt
     4561 aagaatccaa tccaggaagt tcagtccaga ggaatcaata tacatgaaag ttgtttcaaa
     4621 atggcgaaat gtcctatgaa caaaagacct tcacattgga ttgagaagac aagaatacca
     4681 acagagtcac atcaccagtt gaaaagcaaa gcaaaaagat gctcgtcttt ccaaagcaag
     4741 aggactggga cgaattggac agatgatgag aaacgaattt acagggataa aagaatagct
     4801 caaactcagg aaatattaaa gtatttactc cctatcgtgg agtcctctca aaacaggaaa
     4861 tctactcaaa tgaacaatct aatcaacccg atagccaact tgcaacagca tcaacttatt
     4921 ccaactgctt gcataaatag aagaagatac agcatacctt ggacaccaac cagaaatctc
     4981 ccttttaaag cccaaaggaa tcttatgaca gacacctccg aatggctcca gtctgtgccc
     5041 cttgggtcat ttgaaagttt gttacctctc tgcaatcttc agaagaagac aaaacatttt
     5101 gaactctggg gcaaaaaggc taaggatgtg cagcttgcca ccagccaatg ggaggctgtg
     5161 cccttgagtt ctaacatgga tgcaagtatc atcaggggta acagtgttct gcagcctaaa
     5221 gaattcacca tgaggagctc taagcagaga attccttacc taaccaaaac cagcaagaaa
     5281 cccagtgaga gtgatggcct gctcaccttc cagctgaaga ttcacaggaa cagtggatcc
     5341 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     5401 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     5461 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     5521 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5581 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5641 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5701 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5761 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5821 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5881 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5941 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     6001 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     6061 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     6121 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     6181 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     6241 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     6301 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     6361 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     6421 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     6481 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     6541 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     6601 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     6661 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     6721 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6781 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6841 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6901 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6961 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     7021 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     7081 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     7141 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     7201 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     7261 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     7321 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7381 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7441 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7501 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7561 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7621 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7681 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7741 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7801 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7861 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7921 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     7981 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     8041 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     8101 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8161 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8221 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8281 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     8341 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8461 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     8521 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8581 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8641 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
     8701 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8761 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8821 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8881 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8941 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9001 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10861 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10921 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10981 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    11041 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    11101 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    11161 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    11221 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    11281 ttgtaaaacg acggccagtg ccaagctg
//
