LOCUS       V036802                10705 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036802
VERSION     V036802
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4731
                     /label="Ahr(NM_013464)"
                     /note="Ahr(NM_013464)"
                     /gene="Ahr"
     CDS             4738..4803
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4847..5058
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5071..5339
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5368..5377
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5398..6060
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6130..6183
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6184..6783
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6784..7372
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7446..7679
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7751..7872
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7912..8047
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8080..8096)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8104..8120
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8128..8158)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8173..8194
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8482..9070)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9241..10101)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10102..10206)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10680..10696
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gcagcggcgc caacatcacc
     2341 tatgccagcc gcaagcggcg caagccggtg cagaaaacag taaagcccat ccccgctgaa
     2401 ggaattaagt caaatccttc taagcgacac agagaccggc tgaacacaga gttagaccgc
     2461 ctggccagcc tgctgccctt cccgcaagat gttattaata agctggacaa actctctgtt
     2521 cttaggctca gcgtcagcta cctgagggcc aagagcttct ttgatgttgc attaaagtcc
     2581 acccctgctg acagaaatgg aggccaggac cagtgtagag cacaaatcag agactggcag
     2641 gatttgcaag aaggagagtt cttgttacag gcgctgaatg gctttgtgct ggttgtcaca
     2701 gcagatgcct tggtcttcta tgcttcctcc actatccaag attacctggg ctttcagcag
     2761 tctgatgtca tccatcagag cgtatatgag ctcatccata cagaagaccg ggcggaattc
     2821 cagcgccagc ttcactgggc tctaaaccca gactctgcac aaggagtgga cgaagcccat
     2881 ggccctccac aggcagcagt ctattatacc ccagaccagc ttcctccaga gaacgcttct
     2941 ttcatggaga ggtgcttcag gtgccggctg aggtgcctgc tggataattc atctggtttt
     3001 ctggcaatga atttccaagg gaggttaaag tatcttcatg gacagaacaa gaaagggaag
     3061 gacggagcgc tgcttcctcc acaactggct ttgtttgcaa tagctactcc acttcagcca
     3121 ccctccatcc tggaaattcg aaccaaaaac ttcatcttca ggaccaaaca caagctagac
     3181 ttcacaccta ttggttgtga tgccaaaggg cagcttattc tgggctatac agaagtagag
     3241 ctgtgcacaa gaggatcggg gtaccagttc atccatgctg cagacatact tcactgtgca
     3301 gaatcccaca tccgcatgat taagactgga gaaagtggca tgacagtttt ccggcttctt
     3361 gcaaaacaca gtcgctggag gtgggtccag tccaatgcac gcttgattta cagaaatgga
     3421 agaccagatt acatcatcgc cactcagaga ccactgacgg atgaagaagg acgagagcat
     3481 ttacagaagc gaagtacgtc gctgcccttc atgtttgcta ccggagaggc tgtgttgtac
     3541 gagatctcca gccctttctc tcccataatg gatcccctac caatacgcac caaaagcaac
     3601 actagcagga aagactgggc tccccagtca accccaagta aggattcttt ccaccccagt
     3661 tctcttatga gtgccctcat ccagcaggat gagtccatct atctgtgtcc tccttcaagc
     3721 cctgcgctgt tagacagcca ttttctcatg ggctccgtga gcaagtgcgg gagttggcaa
     3781 gacagctttg cggccgcagg aagtgaggct gcgctgaaac atgagcaaat tggccatgct
     3841 caggacgtga accttgcact ctctggcggc ccctcagagc tctttccgga taataaaaat
     3901 aatgacttgt acagcatcat gaggaacctt gggattgatt ttgaagatat cagaagcatg
     3961 cagaacgagg agttcttcag aactgactcc accgctgctg gtgaggttga cttcaaagac
     4021 atcgacataa cggacgaaat cctgacctac gtgcaggatt ccctgaacaa ttcaactttg
     4081 ctgaactcgg cttgccagca gcagcctgtg actcagcacc taagctgtat gctgcaggag
     4141 cgcctgcaac tagagcaaca gcaacagctt cagcagcccc cgccgcaggc tctggagccc
     4201 cagcagcagc tgtgtcagat ggtgtgcccc cagcaagatc tgggtccgaa gcacacgcaa
     4261 atcaacggca cgtttgcaag ttggaacccc acccctcccg tgtctttcaa ctgtccccag
     4321 caggaactaa agcactatca gctcttttcc agcttacagg ggactgctca ggaatttccc
     4381 tacaaaccag aggtggacag tgtgccttac acacagaact ttgctccctg taatcagcct
     4441 ctgcttccag aacattccaa gagtgtgcag ttggacttcc ctggaaggga ttttgaaccg
     4501 tccctgcatc ccactacttc taatttagat tttgtcagtt gtttacaagt tcctgaaaac
     4561 caaagtcatg ggataaactc acagtccgcc atggtcagtc ctcaggcata ctatgctggg
     4621 gccatgtcca tgtatcagtg ccagccaggg ccacagcgca cccctgtgga ccagacgcag
     4681 tacagctctg aaattccagg ttctcaggca ttcctaagca aggtgcagag tggatccgac
     4741 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     4801 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     4861 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     4921 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     4981 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5041 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     5101 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     5161 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     5221 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     5281 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     5341 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     5401 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     5461 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     5521 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     5581 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     5641 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     5701 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     5761 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     5821 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     5881 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     5941 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     6001 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     6061 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     6121 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     6181 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6241 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6301 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6361 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6421 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6481 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6541 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6601 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6661 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6721 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6781 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6961 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7021 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7081 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7141 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7201 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7261 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7321 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     7381 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7441 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     7501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7681 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7741 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7861 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     7921 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7981 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8041 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     8101 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8161 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8221 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8281 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8881 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10201 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10261 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10321 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10381 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10441 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10501 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10561 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10621 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10681 taaaacgacg gccagtgcca agctg
//
