LOCUS       V025896                10114 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025896
VERSION     V025896
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4140
                     /label="Alb(NM_009654)"
                     /note="Alb(NM_009654)"
                     /gene="Alb"
     CDS             4147..4212
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4256..4467
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4480..4748
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4777..4786
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4807..5469
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5539..5592
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5593..6192
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6193..6781
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6855..7088
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7160..7281
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7321..7456
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7489..7505)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7513..7529
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7537..7567)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7582..7603
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7891..8479)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8650..9510)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9511..9615)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10089..10105
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agtgggtaac ctttctcctc
     2341 ctcctcttcg tctccggctc tgctttttcc aggggtgtgt ttcgccgaga agcacacaag
     2401 agtgagatcg cccatcggta taatgatttg ggagaacaac atttcaaagg cctagtcctg
     2461 attgcctttt cccagtatct ccagaaatgc tcatacgatg agcatgccaa attagtgcag
     2521 gaagtaacag actttgcaaa gacgtgtgtt gccgatgagt ctgccgccaa ctgtgacaaa
     2581 tcccttcaca ctctttttgg agataagttg tgtgccattc caaacctccg tgaaaactat
     2641 ggtgaactgg ctgactgctg tacaaaacaa gagcccgaaa gaaacgaatg tttcctgcaa
     2701 cacaaagatg acaaccccag cctgccacca tttgaaaggc cagaggctga ggccatgtgc
     2761 acctccttta aggaaaaccc aaccaccttt atgggacact atttgcatga agttgccaga
     2821 agacatcctt atttctatgc cccagaactt ctttactatg ctgagcagta caatgagatt
     2881 ctgacccagt gttgtgcaga ggctgacaag gaaagctgcc tgaccccgaa gcttgatggt
     2941 gtgaaggaga aagcattggt ctcatctgtc cgtcagagaa tgaagtgctc cagtatgcag
     3001 aagtttggag agagagcttt taaagcatgg gcagtagctc gtctgagcca gacattcccc
     3061 aatgctgact ttgcagaaat caccaaattg gcaacagacc tgaccaaagt caacaaggag
     3121 tgctgccatg gtgacctgct ggaatgcgca gatgacaggg cggaacttgc caagtacatg
     3181 tgtgaaaacc aggcgactat ctccagcaaa ctgcagactt gctgcgataa accactgttg
     3241 aagaaagccc actgtcttag tgaggtggag catgacacca tgcctgctga tctgcctgcc
     3301 attgctgctg attttgttga ggaccaggaa gtgtgcaaga actatgctga ggccaaggat
     3361 gtcttcctgg gcacgttctt gtatgaatat tcaagaagac accctgatta ctctgtatcc
     3421 ctgttgctga gacttgctaa gaaatatgaa gccactctgg aaaagtgctg cgctgaagcc
     3481 aatcctcccg catgctacgg cacagtgctt gctgaatttc agcctcttgt agaagagcct
     3541 aagaacttgg tcaaaaccaa ctgtgatctt tacgagaagc ttggagaata tggattccaa
     3601 aatgccattc tagttcgcta cacccagaaa gcacctcagg tgtcaacccc aactctcgtg
     3661 gaggctgcaa gaaacctagg aagagtgggc accaagtgtt gtacacttcc tgaagatcag
     3721 agactgcctt gtgtggaaga ctatctgtct gcaatcctga accgtgtgtg tctgctgcat
     3781 gagaagaccc cagtgagtga gcatgttacc aagtgctgta gtggatccct ggtggaaagg
     3841 cggccatgct tctctgctct gacagttgat gaaacatatg tccccaaaga gtttaaagct
     3901 gagaccttca ccttccactc tgatatctgc acacttccag agaaggagaa gcagattaag
     3961 aaacaaacgg ctcttgctga gctggtgaag cacaagccca aggctacagc ggagcaactg
     4021 aagactgtca tggatgactt tgcacagttc ctggatacat gttgcaaggc tgctgacaag
     4081 gacacctgct tctcgactga gggtccaaac cttgtcacta gatgcaaaga cgccttagcc
     4141 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4201 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4261 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4321 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4381 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4441 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4501 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4561 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     4621 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     4681 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     4741 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     4801 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     4861 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     4921 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     4981 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5041 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5101 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5161 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5221 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5281 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5341 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5401 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5461 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     5521 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     5581 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5641 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5701 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5761 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5821 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5881 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5941 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6001 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6061 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6121 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6181 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6241 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6301 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6361 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6421 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6481 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6541 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6601 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6661 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6721 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6781 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6841 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     6901 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6961 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7021 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7081 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7141 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7261 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7381 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7441 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     7501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9661 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     9721 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     9781 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     9841 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9901 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     9961 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10021 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10081 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
