LOCUS       V045791                10534 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045791
VERSION     V045791
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4560
                     /label="Ankzf1(NM_026187)"
                     /note="Ankzf1(NM_026187)"
                     /gene="Ankzf1"
     CDS             4567..4632
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4676..4887
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4900..5168
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5197..5206
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5227..5889
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5959..6012
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6013..6612
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6613..7201
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7275..7508
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7580..7701
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7741..7876
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7909..7925)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7933..7949
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7957..7987)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8002..8023
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8311..8899)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9070..9930)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9931..10035)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10509..10525
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccaaaccggc tggcgatgcg
     2341 gcagtaggat ctcgttctgg agagttgttt ctgccctccg tttcttcttc agccacgtcg
     2401 ccagctccaa gtgcagcctc agcccctgcc tccgtttccc tgttgtccct caacggggaa
     2461 gctccactca ttcggggcct gagcctggtg agccaagctc ctggggaggc tctggcctgg
     2521 gcgccgcgta cttcctgtcc aggagagaac acaagctcgg ggggaaaagt atctccatat
     2581 tctctggaaa tttcagagaa attattttgt tcagcctgtg accagatctt ccagaaccac
     2641 caggaacaga gagaacatta taagcttgat tggcatcgct ttaacttaaa acaacgtctc
     2701 aagaacaagc ctctcctgtc tgcttcagac tttgaacagc aaagctccac aggggatctt
     2761 tccagcatct caggatcaga tgacacagac tccagcagtg aagaggactt gctgccactg
     2821 gatgagggga gggctgaatc cgagaaacct aaccggcccc ccggattcta cccacatcgg
     2881 gtccttttca aaaacgccca gggccagttt ctttatgcct atcgttgtgt cctaggccct
     2941 caccagattc ccccagaaaa ggcagaattg ctactacaaa acctacagaa tggaggtccc
     3001 agatactacg tggtgctcat ggctgcggct ggacattttg ctggtgccat ttttcaagga
     3061 agagaagtgg tggcacacaa aacctttcac cgttacactg tgcgggccaa gcggggcaca
     3121 gcccaggggc ttcaggatgc ccatggtagg gcatcccgct ctgctggagc caacttgagg
     3181 cgttacaatg aagccatgct ctataaggat gttcgcaacc tgctggcggg gccaatctgg
     3241 tccaaggcac taggggaggc agaaacagta ttgttccgag caccacgctc tggccggtcc
     3301 ttgttctttg gaggccaagg ggcaccccta caaagggatg atccccgact ttgggatatc
     3361 cctctcacca cccgcagacc cacttttgga gagctccagc gtgtactcca taagctgacc
     3421 accttgcagg tgtatgatga agaccctcga gaaatggtca gatttcactc tcctgagaca
     3481 cactggaaac cagtgagaga agagaggaaa aaggatactg agaaagaaaa aacaaaggtc
     3541 cccagtgatg caaataagcc ccttgggcag gatgaagaac cactcaaaca aggttcagag
     3601 tctcaggaag aagatggttc cgaagtagag ttggagctag tagaattgac cctggggacc
     3661 ctggatcttc gtgaatttga agtactgccc aagcggagga ggaggaggaa gaagaaggag
     3721 agaagtcaag agcagcagtg tggggcacat gggcctcttc ctcagcaacc tcaagatgag
     3781 ccgttctcac agccaaccca agaggttgaa accccattgg atactttggt ctatgaggcc
     3841 aaagcccctg gtcagccaga gctctgggac acgcttctag ctgcttgtcg tgctggggaa
     3901 gttgaggtgc taaagctgca gctagccact gggctggtag accctggagt taagtccctg
     3961 ctcaatgccc ctttgggctc cggtggcttt actctcctgc atgcagcagc cgcagctgga
     4021 agaggcttag tagttcgttt gctgttggaa gcaggtgctg atcctactgt gcaggactct
     4081 agggctcggc caccttatac tgtagcagct gacaaatcaa cgcgtaatga attccggagg
     4141 ttcatggaga agaatcttga tgcctatgat tacaacaagg ctcgggtgcc agggccattg
     4201 actcaagaaa tggaggcccg gcaggctaca aggaaaaaag agcagaaggc agctcgacgg
     4261 caacgggagc aacagcagcg gaagcagagg gagcaggaag aacaagagca agaggaacaa
     4321 cggcggtttg ctgccctcag tgaccgggag aagagagctc tggctgcaga gcgccgactt
     4381 gctgcccagt tgggagcccc tagccctcca gttcctgact ctgcagttgc tagtgctgga
     4441 cgctgctgga gttgtggagt gtccctccaa ggcctcattc cctttcacta cctcgacttc
     4501 tctttctgct ccacacgttg ccttcgagac catcgcagtc aggcggggag accttcatct
     4561 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4621 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4681 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4741 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4801 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4861 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4921 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4981 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     5041 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     5101 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5161 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5221 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5281 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5341 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5401 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5461 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5521 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5581 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5641 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5701 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5761 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5821 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5881 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     5941 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     6001 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6061 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6121 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6181 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6241 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6301 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6361 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6421 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6481 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6541 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6601 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6661 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6721 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6781 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6841 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6901 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6961 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7021 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7081 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7141 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7201 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7261 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     7321 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7381 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7441 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7501 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7561 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7621 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7681 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7801 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7861 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     7921 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7981 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8041 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8101 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8941 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9001 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10081 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10141 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10201 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10261 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10321 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10381 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10441 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10501 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
