LOCUS       V037761                10895 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037761
VERSION     V037761
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4980
                     /label="Axl(NM_009465)"
                     /note="Axl(NM_009465)"
                     /gene="Axl"
     CDS             4987..5010
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        5054..5265
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5278..5546
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5575..5584
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5605..6267
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6337..6390
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6391..6990
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6991..7578
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7652..7885
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7957..8078
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8097..8232
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8270..8286)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8294..8310
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8318..8348)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8363..8384
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8672..9260)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9431..10291)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10292..10396)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10870..10886
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gcagggtccc gctggcctgg
     2341 tggttggcgc tgtgctgctg ggggtgtgca gcccataagg acacacagac cgaggctggc
     2401 agcccgtttg tggggaaccc agggaatatc acaggtgcca gaggactcac ggggacactt
     2461 cggtgtgagc tccaggttca gggggaaccc cctgaggtgg tgtggcttcg agatggacag
     2521 atcctagaac tggctgataa cacccagacc caggtgcctc tgggcgaaga ctggcaagat
     2581 gaatggaaag ttgtcagtca gctcagaatc tcagccctgc aactttcaga tgcaggggag
     2641 taccagtgta tggtgcatct agaaggacgg acctttgtgt ctcagccggg ctttgtaggg
     2701 ctggaaggtc tcccgtactt cctggaggag cctgaggaca aagctgtgcc tgccaacacc
     2761 cctttcaacc taagctgcca ggcccaggga cccccggaac ccgtgaccct actctggctt
     2821 caagatgctg tccccctggc cccagtcaca ggacacagct cccagcacag tctgcaaact
     2881 ccaggcctga acaagacatc ttctttctca tgtgaagccc acaatgccaa gggagtcacc
     2941 acctcccgca cagccaccat cacagtgctc ccccagaggc ctcaccatct ccacgtggtt
     3001 tccagacaac ctacggagct agaggtagct tggacccctg gcctgagtgg catctacccg
     3061 ctcacccact gcaacctgca ggccgtgctg tcagacgatg gggtgggtat ctggctggga
     3121 aagtcagatc ctcctgaaga ccccctcacc ttgcaagtat cagtgccccc ccaccagctt
     3181 cggctggaaa agctccttcc tcacaccccg tatcacatcc ggatatcctg cagcagcagc
     3241 cagggcccct caccttggac ccactggctt cctgtggaga ccacagaggg agtgcccttg
     3301 ggtccccctg agaacgttag cgccatgcgg aatgggagcc aggtcctcgt gcgttggcag
     3361 gagccaaggg tgcccctgca aggcaccctg ttagggtacc ggctggcata tcgaggccag
     3421 gacacccccg aggtacttat ggatataggg ctaactcgag aggtgacctt ggaactgcgg
     3481 ggggacaggc ctgtggctaa cctgactgtg tctgtgacag cctatacctc ggctggggat
     3541 gggccctgga gccttcctgt gcccctagag ccctggcgcc cagggcaagg acagccactc
     3601 caccatctgg tgagtgaacc cccacctcgc gccttctcgt ggccttggtg gtatgtactg
     3661 ctgggagcac ttgtggctgc cgcctgcgtc ctcatcttgg ccctgttcct tgtccatcgg
     3721 aggaagaagg agactcgata tggggaggtg tttgagccaa ccgtggaaag aggtgaactg
     3781 gtagtcaggt accgtgtccg aaagtcctac agccggcgga ccactgaagc caccttgaac
     3841 agtctgggca tcagtgaaga gctgaaggag aaactacgag acgtcatggt agatcggcat
     3901 aaggtggcct tggggaagac cctgggagaa ggagaatttg gcgctgtgat ggaaggtcag
     3961 ctcaatcagg atgactccat cctcaaggtc gctgtgaaga ccatgaaaat tgccatctgc
     4021 acaagatcag agctggagga tttcctgagt gaagctgtct gcatgaagga atttgaccac
     4081 cccaacgtca tgaggctcat tggcgtctgt tttcagggct ctgacagaga gggtttccca
     4141 gaacctgtgg tcatcttgcc tttcatgaaa cacggagacc tacacagttt cctcctgtac
     4201 tcccggctcg gggaccagcc agtgttcctg cccactcaga tgctagtgaa gttcatggcc
     4261 gacattgcca gtggtatgga gtacctgagt accaagagat tcatacatcg ggacctggct
     4321 gccaggaact gcatgctgaa tgagaacatg tccgtgtgtg tggcagactt cgggctctcc
     4381 aagaagatct acaacgggga ttactaccgc caagggcgca ttgccaagat gccagtcaag
     4441 tggattgcta ttgagagtct ggcagatcgg gtctacacca gcaagagcga tgtgtggtcc
     4501 ttcggtgtga caatgtggga gatcgccacc cgaggccaaa ctccctatcc aggggtggag
     4561 aacagtgaga tttacgacta cctgcgtcaa ggaaatcggc tgaaacagcc tgtggactgt
     4621 ctggacggcc tgtatgccct gatgtctcgg tgctgggaac tgaaccctcg agaccggcca
     4681 agttttgcgg agctccggga agacttggag aacacactga aggctctgcc ccctgctcag
     4741 gagccagatg aaatcctcta tgtcaacatg gatgagggcg gaagccacct tgaaccccgt
     4801 ggggctgctg gaggagctga ccccccaacc caacctgatc ctaaggattc ctgtagctgt
     4861 ctcactgcag ctgacgtcca ctcagctgga cgctatgtcc tttgtccttc tacagcccca
     4921 ggacccactc tgtctgctga cagaggctgc ccagcacctc cagggcagga ggacggagcc
     4981 ggatcccacc atcaccatca ccatcaccat tagcggccgc gaaggatctg cgatcgctcc
     5041 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5101 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5161 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5221 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5281 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5341 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5401 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5461 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5521 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5581 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     5641 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     5701 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     5761 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     5821 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     5881 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     5941 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     6001 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     6061 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     6121 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6181 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     6241 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     6301 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     6361 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     6421 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6481 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6541 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6601 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6661 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6721 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6781 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6841 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6901 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6961 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     7021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7381 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7501 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc
     7561 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7621 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7681 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7741 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7801 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7861 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7921 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8101 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8161 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8221 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     8281 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8341 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8401 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8461 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8521 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8581 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8641 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8701 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8761 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8821 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8881 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8941 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9001 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9061 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9301 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9361 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9421 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9481 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9541 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9601 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9721 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9781 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9841 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9901 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9961 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10021 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10081 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10141 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10201 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10261 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10321 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10381 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10441 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10501 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10561 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10621 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10681 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10741 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10801 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10861 cacgacgttg taaaacgacg gccagtgcca agctg
//
