LOCUS       V042041                10121 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042041
VERSION     V042041
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4953
                     /label="Bcl11b(NM_001079883)"
                     /note="Bcl11b(NM_001079883)"
                     /gene="Bcl11b"
     CDS             4960..4986
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4990..5016
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5020..5046
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5091..5302
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5315..5583
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5610..6209
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6216..6804
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6878..7111
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7183..7304
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7323..7458
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7496..7512)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7520..7536
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7544..7574)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7589..7610
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7898..8486)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8657..9517)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9518..9622)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10096..10112
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtcccgc cgcaaacagg gcaacccgca gcacttgtcc
     2341 cagagggaac tcatcacgcc agaggctgac catgtggagg ctaccatcct cgaggaagac
     2401 gagggtctgg agatagagga gcctagcagc ctggggctga tggtgggagg ccccgaccct
     2461 gatctactca cctgtggcca gtgtcagatg aacttcccgc tgggggacat cctggttttt
     2521 atagagcaca agaagaaaca gtgtggaggc ctgggcccct gctacgacaa ggtcctggac
     2581 aagagcagtc cacctccctc ctctcgctct gagctcagga gagtatctga gccagtggag
     2641 atcgggatcc aggtcacccc tgatgaagat gaccacctac tgtcacccac gaaaggcatc
     2701 tgtcccaagc aggagaacat tgcagggccg tgcaggcctg cccagctgcc atcgatggcc
     2761 cccatagctg cctcctcttc ccaccctccc acctccgtga ttacttcacc tctgcgtgcc
     2821 ctgggcgttc tcccaccctg tttcccgctg ccttgctgtg gtgcacgccc catctcgggc
     2881 gacgggactc agggtgaggg tcagatggag gctccctttg gatgccagtg tgagttgtca
     2941 ggtaaagatg agccttccag ctacatttgc acaacatgca agcagccctt caacagcgcc
     3001 tggttcctgc tgcagcacgc acagaacaca catggcttcc gaatctacct ggagcctggg
     3061 ccggccagca cctcgctcac gcccaggctc accatcccgc caccgctcgg gccggagacc
     3121 gtggcgcagt ccccactcat gaatttcctg ggggacagca atcctttcaa cctgctgcgc
     3181 atgacgggcc ccatcctgcg ggaccaccct ggcttcggtg agggccgctt gccaggtacg
     3241 ccaccgctct tcagcccacc gccacgccat cacttggacc cacaccgcct cagtgcagag
     3301 gagatggggc tcgtggccca gcaccccagt gccttcgacc gagtcatgcg cctgaacccc
     3361 atggccatag actctcctgc catggacttc tcccggcggc tgcgagaact ggccggcaac
     3421 agctccacgc cgccgcccgt gtccccaggc cgtggcaacc ctatgcaccg gctgctgaac
     3481 cctttccagc ccagtcccaa gtccccgttc ctcagcacgc caccgctgcc acccatgcct
     3541 gcgggcacac cgccaccgca gccgcctgcc aagagcaagt cctgtgagtt ctgcggcaag
     3601 accttcaagt tccagagcaa tctcatcgtg caccggcgca gccacacggg cgagaagccc
     3661 tacaagtgcc agctgtgcga ccatgcgtgc tcgcaggcga gcaagctcaa gcgccacatg
     3721 aagacgcaca tgcacaaggc gggctctctg gctggccgct cagacgacgg gctctcagct
     3781 gccagctccc ctgagccggg caccagcgag ctgccaggtg acctgaaagc ggccgatggc
     3841 gacttccgcc accatgagag cgacccatct ctgggccccg agcctgagga cgacgaggac
     3901 gaggaggagg aagaagagga gctgctgctg gagaacgaga gccggcctga gtcgagcttc
     3961 agcatggact cggagctggg ccgtggccgc gagaacggag gtggcgtgcc accgggggtg
     4021 gcgggcgcag gggctgcagc tgcggctctg gcggatgaga aggctctggc cctgggcaag
     4081 gtgatggagg acgcagggct gggcgcactg ccgcagtatg gggagaagcg gggcgccttc
     4141 ctgaagcgtg caggcgacac gggtgatgcc ggagctgttg gctgtgggga cgcgggtgca
     4201 ccgggtgcag tgaacgggcg cggcggggcc ttcgcgccag gcgcagagcc ctttccagct
     4261 ctcttcccac gcaagccagc accgctgccc agccctgggc tcggtggtcc cgcgctgcac
     4321 gcggccaagc gcatcaaggt ggagaaagac ctggagctgc cacctgccgc cctcatccca
     4381 tctgagaacg tgtactcgca gtggctcgtg ggctacgcag catcgcgcca cttcatgaag
     4441 gacccattcc tgggcttcac ggatgcgcgc cagtcgcctt tcgccacatc gtcggaacat
     4501 tcctctgaga acggcagcct gcgcttctca acgccacccg gggacctgct ggacggcggg
     4561 ctgtccgggc gcagtggcac ggcgagcggg ggcagcacac ctcacctggg tggtccgggt
     4621 cctgggaggc cgagctccaa ggagggccgc cgcagcgaca catgtgagta ctgcggcaag
     4681 gtcttcaaga actgtagcaa cctgacggtg caccggagga gccacaccgg cgagcggcct
     4741 tacaagtgcg agctgtgcaa ctacgcgtgc gcgcagagca gcaagctcac gcgccacatg
     4801 aagacgcacg ggcagatcgg caaggaggtg taccgctgcg acatctgcca gatgcccttc
     4861 agcgtctaca gcaccctgga gaaacacatg aaaaagtggc acggtgaaca cttgctgact
     4921 aatgatgtca aaatcgagca ggctgagagg agcggatcct acccatacga cgtacctgat
     4981 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     5041 tacgcatagg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     5101 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     5161 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     5221 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     5281 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     5341 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     5401 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     5461 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     5521 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     5581 atccaagctg tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg
     5641 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5701 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     5761 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5821 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     5881 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     5941 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6001 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6061 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6121 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6181 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt
     6241 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6301 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6361 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6421 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6481 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6541 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6601 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6661 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6721 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6781 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     6841 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6901 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6961 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7021 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7081 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7141 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     7201 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7261 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     7321 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7381 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7441 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     7501 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7561 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7621 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7681 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8461 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8521 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8581 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8641 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8701 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8761 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8821 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8881 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8941 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9001 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9061 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9121 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9181 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9241 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9301 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9361 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9421 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9481 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9541 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9601 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9661 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     9721 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     9781 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     9841 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     9901 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     9961 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    10021 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    10081 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
