LOCUS V039014 8984 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039014 VERSION V039014 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3816 /label="CAMKK1(NM_032294)" /note="CAMKK1(NM_032294)" /gene="CAMKK1" CDS 3823..3849 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3853..3879 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3883..3909 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3954..4165 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4178..4446 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4473..5072 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5079..5667 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5741..5974 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6046..6167 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6186..6321 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6359..6375) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6383..6399 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6407..6437) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6452..6473 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6761..7349) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7520..8380) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8381..8485) /gene="bla" /label="AmpR promoter" primer_bind 8959..8975 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggagggg ggtccagctg tctgctgcca ggatcctcgg 2341 gcagagctgg tagaacgggt ggcagccatc gatgtgactc acttggagga ggcagatggt 2401 ggcccagagc ctactagaaa cggtgtggac cccccaccac gggccagagc tgcctctgtg 2461 atccctggca gtacttcaag actgctccca gcccggccta gcctctcagc caggaagctt 2521 tccctacagg agcggccagc aggaagctat ctggaggcgc aggctgggcc ttatgccacg 2581 gggcctgcca gccacatctc cccccgggcc tggcggaggc ccaccatcga gtcccaccac 2641 gtggccatct cagatgcaga ggactgcgtg cagctgaacc agtacaagct gcagagtgag 2701 attggcaagg gtgcctacgg tgtggtgagg ctggcctaca acgaaagtga agacagacac 2761 tatgcaatga aagtcctttc caaaaagaag ttactgaagc agtatggctt tccacgtcgc 2821 cctcccccga gagggtccca ggctgcccag ggaggaccag ccaagcagct gctgcccctg 2881 gagcgggtgt accaggagat tgccatcctg aagaagctgg accacgtgaa tgtggtcaaa 2941 ctgatcgagg tcctggatga cccagctgag gacaacctct atttggtgtt tgacctcctg 3001 agaaaggggc ccgtcatgga agtgccctgt gacaagccct tctcggagga gcaagctcgc 3061 ctctacctgc gggacgtcat cctgggcctc gagtacttgc actgccagaa gatcgtccac 3121 agggacatca agccatccaa cctgctcctg ggggatgatg ggcacgtgaa gatcgccgac 3181 tttggcgtca gcaaccagtt tgaggggaac gacgctcagc tgtccagcac ggcgggaacc 3241 ccagcattca tggcccccga ggccatttct gattccggcc agagcttcag tgggaaggcc 3301 ttggatgtat gggccactgg cgtcacgttg tactgctttg tctatgggaa gtgcccattc 3361 atcgacgatt tcatcctggc cctccacagg aagatcaaga atgagcccgt ggtgtttcct 3421 gaggagccag aaatcagcga ggagctcaag gacctgatcc tgaagatgtt agacaagaat 3481 cccgagacga gaattggggt gccagacatc aagttgcacc cttgggtgac caagaacggg 3541 gaggagcccc ttccttcgga ggaggagcac tgcagcgtgg tggaggtgac agaggaggag 3601 gttaagaact cagtcaggct catccccagc tggaccacgg tgatcctggt gaagtccatg 3661 ctgaggaagc gttcctttgg gaacccgttt gagccccaag cacggaggga agagcgatcc 3721 atgtctgctc caggaaacct actggtgaaa gaagggtttg gtgaaggggg caagagccca 3781 gagctccccg gcgtccagga agacgaggct gcatccggat cctacccata cgacgtacct 3841 gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta tgatgtccca 3901 gactacgcat aggcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga 3961 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc 4021 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 4081 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 4141 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg 4201 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc 4261 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag 4321 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct 4381 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta 4441 cagatccaag ctgtgaccgg cgcctacgct agatgaccga gtacaagccc acggtgcgcc 4501 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 4561 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 4621 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 4681 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 4741 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 4801 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 4861 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 4921 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 4981 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 5041 cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa 5101 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 5161 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 5221 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 5281 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 5341 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 5401 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 5461 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 5521 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 5581 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 5641 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca 5701 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 5761 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 5821 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 5881 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 5941 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6001 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 6061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 6121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 6181 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 6241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 6301 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt 6361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 6721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 6781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 6961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 7141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7201 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7321 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7441 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7501 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7561 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7621 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 7681 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 7741 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 7801 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 7861 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 7921 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 7981 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8041 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8101 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 8161 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 8221 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8281 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8341 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8401 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8461 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 8521 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 8581 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 8641 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 8701 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 8761 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 8821 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 8881 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 8941 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg //