LOCUS       V041875                11980 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041875
VERSION     V041875
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6006
                     /label="CAND1(NM_018448)"
                     /note="CAND1(NM_018448)"
                     /gene="CAND1"
     CDS             6013..6078
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6122..6333
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6346..6614
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6643..6652
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6673..7335
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7405..7458
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7459..8058
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8059..8647
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8721..8954
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9026..9147
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9187..9322
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9355..9371)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9379..9395
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9403..9433)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9448..9469
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9757..10345)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10516..11376)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11377..11481)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11955..11971
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgagcgcctc gtaccacatt
     2341 tccaatttgc tggaaaaaat gacatccagc gacaaggact ttaggtttat ggctacaaat
     2401 gatttgatga cggaactgca gaaagattcc atcaagttgg atgatgatag tgaaaggaaa
     2461 gtagtgaaaa tgattttgaa gttattggaa gataaaaatg gagaggtaca gaatttagct
     2521 gtcaaatgtc ttggtccttt agtgagtaaa gtgaaagaat accaagtaga gacaattgta
     2581 gataccctct gcactaacat gctttctgat aaagaacaac ttcgagacat ttcaagtatt
     2641 ggtcttaaaa cagtaattgg agaacttcct ccagcttcca gtggctctgc attagctgct
     2701 aatgtatgta aaaagattac tggacgtctt acaagtgcaa tagcaaaaca ggaagatgtc
     2761 tctgttcagc tagaagcctt ggatattatg gctgatatgt tgagcaggca aggaggactt
     2821 cttgttaatt tccatccttc aattctgacc tgtctacttc cccagttgac cagccctaga
     2881 cttgcagtga ggaaaagaac cattatcgct cttggccatc tggttatgag ctgtggaaat
     2941 atagtttttg tagatcttat tgaacatctg ttgtcagagt tgtccaaaaa tgattctatg
     3001 tcaacaacaa gaacctacat acaatgtatt gctgctatta gtaggcaagc tggtcataga
     3061 ataggtgaat accttgagaa gataattcct ttggtggtaa aattttgcaa tgtagatgat
     3121 gatgaattaa gagagtactg tattcaagcc tttgaatcat ttgtaagaag atgtcctaag
     3181 gaagtatatc ctcatgtttc taccattata aatatttgtc ttaaatatct tacctatgat
     3241 ccaaattata attacgatga tgaagatgaa gatgaaaatg caatggatgc tgatggtggt
     3301 gatgatgatg atcaagggag tgatgatgaa tacagtgatg atgatgacat gagttggaaa
     3361 gtgagacgtg cagctgcgaa gtgcttggat gctgtagtta gcacaaggca tgaaatgctt
     3421 ccagaattct acaagaccgt ctctcctgca ctaatatcca gatttaaaga gcgtgaagag
     3481 aatgtaaagg cagatgtttt tcacgcatac ctttctcttt tgaagcaaac tcgtcctgta
     3541 caaagttggc tatgtgaccc tgatgcaatg gagcagggag aaacaccttt aacaatgctt
     3601 cagagtcagg ttcccaacat tgttaaagct cttcacaaac agatgaaaga aaaaagtgtg
     3661 aagacccgac agtgttgttt taacatgtta actgagctgg taaatgtatt acctggggcc
     3721 ctaactcaac acattcctgt acttgtacca ggaatcattt tctcactgaa tgataaatca
     3781 agctcatcga atttgaagat cgatgctttg tcatgtctat acgtaatcct ctgtaaccat
     3841 tctcctcaag tcttccatcc tcacgttcag gctttggttc ctccagtggt ggcttgtgtt
     3901 ggagacccat tttacaaaat tacatctgaa gcacttcttg ttactcaaca gcttgtcaaa
     3961 gtaattcgtc ctttagatca gccttcctcg tttgatgcaa ctccttatat caaagatcta
     4021 tttacctgta ccattaagag attaaaagca gctgacattg atcaggaagt caaggaaagg
     4081 gctatttcct gtatgggaca aattatttgc aaccttggag acaatttggg ttctgacttg
     4141 cctaatacac ttcagatttt cttggagaga ctaaagaatg aaattaccag gttaactaca
     4201 gtaaaggcat tgacactgat tgctgggtca cctttgaaga tagatttgag gcctgttctg
     4261 ggagaagggg ttcctatcct tgcttcattt cttagaaaaa accagagagc tttgaaactg
     4321 ggtactcttt ctgcccttga tattctaata aaaaactata gtgacagctt gacagctgcc
     4381 atgattgatg cagttctaga tgagctccca cctcttatca gcgaaagtga tatgcatgtt
     4441 tcacaaatgg ccatcagttt tcttaccact ttggcaaaag tatatccctc ctccctttca
     4501 aagataagtg gatccattct caatgaactt attggacttg tgagatcacc cttattgcag
     4561 gggggagctc ttagtgccat gctagacttt ttccaagctc tggttgtcac tggaacaaat
     4621 aatttaggat acatggattt gttgcgcatg ctgactggtc cagtttactc tcagagcaca
     4681 gctcttactc ataagcagtc ttattattcc attgccaaat gtgtagctgc ccttactcga
     4741 gcatgcccta aagagggacc agctgtagta ggtcagttta ttcaagatgt caagaactca
     4801 aggtctacag attccattcg tctcttagct ctactttctc ttggagaagt tgggcatcat
     4861 attgacttaa gtggacagtt ggaactaaaa tctgtaatac tagaagcttt ctcatctcct
     4921 agtgaagaag tcaaatcagc tgcatcctat gcattaggca gcattagtgt gggcaacctt
     4981 cctgaatatc tgccgtttgt cctgcaagaa ataactagtc aacccaaaag gcagtatctt
     5041 ttacttcatt ccttgaagga aattattagc tctgcatcag tggtgggcct taaaccatat
     5101 gttgaaaaca tctgggcctt attactaaag cactgtgagt gtgcagagga aggaaccaga
     5161 aatgttgttg ctgaatgtct aggaaaactc actctaattg atccagaaac tctccttcca
     5221 cggcttaagg ggtacttgat atcaggctca tcatatgccc gaagctcagt ggttacggct
     5281 gtgaaattta caatttctga ccatccacaa cctattgatc cactgttaaa gaactgcata
     5341 ggtgatttcc taaaaacttt ggaagaccca gatttgaatg tgagaagagt agccttggtc
     5401 acatttaatt cagcagcaca taacaagcca tcattaataa gggatctatt ggatactgtt
     5461 cttccacatc tttacaatga aacaaaagtt agaaaggagc ttataagaga ggtagaaatg
     5521 ggtccattta aacatacggt tgatgatggt ctggatatta gaaaggcagc atttgagtgt
     5581 atgtacacac ttctagacag ttgtcttgat agacttgata tctttgaatt tctaaatcat
     5641 gttgaagatg gtttgaagga ccattatgat attaagatgc tgacattttt aatgttggtg
     5701 agactgtcta ccctttgtcc aagtgcagta ctgcagaggt tggaccgact tgttgagcca
     5761 ttacgtgcaa catgtacaac taaggtaaag gcaaactcag taaagcagga gtttgaaaaa
     5821 caagatgaat taaagcgatc tgccatgaga gcagtagcag cactgctaac cattccagaa
     5881 gcagagaaga gtccactgat gagtgaattc cagtcacaga tcagttctaa ccctgagctg
     5941 gcggctatct ttgaaagtat ccagaaagat tcatcatcta ctaacttgga atcaatggac
     6001 actagtggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6061 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6121 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6181 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6241 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6301 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     6361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     6421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6601 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6661 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6721 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6781 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6841 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6901 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6961 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     7021 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     7081 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     7141 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     7201 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     7261 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     7321 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     7381 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     7441 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7501 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7561 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7621 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7681 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7741 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7801 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7861 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7921 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7981 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     8041 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8101 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8161 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8221 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8281 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8341 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8401 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8461 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8521 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8581 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8641 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8701 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     8761 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8821 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8881 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8941 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     9001 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     9061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9121 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     9181 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9241 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9301 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     9361 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9421 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9481 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    10201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    11521 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    11581 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    11641 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11701 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11761 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    11821 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11881 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11941 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
