LOCUS       V036256                11320 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036256
VERSION     V036256
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5346
                     /label="CBLB(NM_001321786)"
                     /note="CBLB(NM_001321786)"
                     /gene="CBLB"
     CDS             5353..5418
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5462..5673
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5686..5954
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5983..5992
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6013..6675
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6745..6798
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6799..7398
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7399..7987
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8061..8294
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8366..8487
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8527..8662
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8695..8711)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8719..8735
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8743..8773)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8788..8809
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9097..9685)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9856..10716)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10717..10821)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11295..11311
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gctatttgtg tgttaatttc
     2341 atttggttct tgggaataac gactcaccgc gttgatttaa agaaagaact aaaattccag
     2401 atggcaaact caatgaatgg cagaaaccct ggtggtcgag gaggaaatcc ccgaaaaggt
     2461 cgaattttgg gtattattga tgctattcag gatgcagttg gaccccctaa gcaagctgcc
     2521 gcagatcgca ggaccgtgga gaagacttgg aagctcatgg acaaagtggt aagactgtgc
     2581 caaaatccca aacttcagtt gaaaaatagc ccaccatata tacttgatat tttgcctgat
     2641 acatatcagc atttacgact tatattgagt aaatatgatg acaaccagaa acttgcccaa
     2701 ctcagtgaga atgagtactt taaaatctac attgatagcc ttatgaaaaa gtcaaaacgg
     2761 gcaataagac tctttaaaga aggcaaggag agaatgtatg aagaacagtc acaggacaga
     2821 cgaaatctca caaaactgtc ccttatcttc agtcacatgc tggcagaaat caaagcaatc
     2881 tttcccaatg gtcaattcca gggagataac tttcgtatca caaaagcaga tgctgctgaa
     2941 ttctggagaa agttttttgg agacaaaact atcgtaccat ggaaagtatt cagacagtgc
     3001 cttcatgagg tccaccagat tagctctggc ctggaagcaa tggctctaaa atcaacaatt
     3061 gatttaactt gcaatgatta catttcagtt tttgaatttg atatttttac caggctgttt
     3121 cagccttggg gctctatttt gcggaattgg aatttcttag ctgtgacaca tccaggttac
     3181 atggcatttc tcacatatga tgaagttaaa gcacgactac agaaatatag caccaaaccc
     3241 ggaagctata ttttccggtt aagttgcact cgattgggac agtgggccat tggctatgtg
     3301 actggggatg ggaatatctt acagaccata cctcataaca agcccttatt tcaagccctg
     3361 attgatggca gcagggaagg attttatctt tatcctgatg ggaggagtta taatcctgat
     3421 ttaactggat tatgtgaacc tacacctcat gaccatataa aagttacaca ggaacaatat
     3481 gaattatatt gtgaaatggg ctccactttt cagctctgta agatttgtgc agagaatgac
     3541 aaagatgtca agattgagcc ttgtgggcat ttgatgtgca cctcttgcct tacggcatgg
     3601 caggagtcgg atggtcaggg ctgccctttc tgtcgttgtg aaataaaagg aactgagccc
     3661 ataatcgtgg acccctttga tccaagagat gaaggctcca ggtgttgcag catcattgac
     3721 ccctttggca tgccgatgct agacttggac gacgatgatg atcgtgagga gtccttgatg
     3781 atgaatcggt tggcaaacgt ccgaaagtgc actgacaggc agaactcacc agtcacatca
     3841 ccaggatcct ctccccttgc ccagagaaga aagccacagc ctgacccact ccagatccca
     3901 catctaagcc tgccacccgt gcctcctcgc ctggatctaa ttcagaaagg catagttaga
     3961 tctccctgtg gcagcccaac gggttcacca aagtcttctc cttgcatggt gagaaaacaa
     4021 gataaaccac tcccagcacc acctcctccc ttaagagatc ctcctccacc gccacctgaa
     4081 agacctccac caatcccacc agacaataga ctgagtagac acatccatca tgtggaaagc
     4141 gtgccttcca gagacccgcc aatgcctctt gaagcatggt gccctcggga tgtgtttggg
     4201 actaatcagc ttgtgggatg tcgactccta ggggagggct ctccaaaacc tggaatcaca
     4261 gcgagttcaa atgtcaatgg aaggcacagt agagtgggct ctgacccagt gcttatgcgg
     4321 aaacacagac gccatgattt gcctttagaa ggagctaagg tcttttccaa tggtcacctt
     4381 ggaagtgaag aatatgatgt tcctccccgg ctttctcctc ctcctccagt taccaccctc
     4441 ctccctagca taaagtgtac tggtccgtta gcaaattctc tttcagagaa aacaagagac
     4501 ccagtagagg aagatgatga tgaatacaag attccttcat cccaccctgt ttccctgaat
     4561 tcacaaccat ctcattgtca taatgtaaaa cctcctgttc ggtcttgtga taatggtcac
     4621 tgtatgctga atggaacaca tggtccatct tcagagaaga aatcaaacat ccctgactta
     4681 agcatatatt taaagggaga tgtttttgat tcagcctctg atcccgtgcc attaccacct
     4741 gccaggcctc caactcggga caatccaaag catggttctt cactcaacag gacgccctct
     4801 gattatgatc ttctcatccc tccattaggt gaagatgctt ttgatgccct ccctccatct
     4861 ctcccacctc ccccacctcc tgcaaggcat agtctcattg aacattcaaa acctcctggc
     4921 tccagtagcc ggccatcctc aggacaggat ctttttcttc ttccttcaga tccctttgtt
     4981 gatctagcaa gtggccaagt tcctttgcct cctgctagaa ggttaccagg tgaaaatgtc
     5041 aaaactaaca gaacatcaca ggactatgat cagcttcctt catgttcaga tggttcacag
     5101 gcaccagcca gaccccctaa accacgaccg cgcaggactg caccagaaat tcaccacaga
     5161 aaaccccatg ggcctgaggc ggcattggaa aatgtcgatg caaaaattgc aaaactcatg
     5221 ggagagggtt atgcctttga agaggtgaag agagccttag agatagccca gaataatgtc
     5281 gaagttgccc ggagcatcct ccgagaattt gccttccctc ctccagtatc cccacgtcta
     5341 aatctaggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     5401 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5461 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5521 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5581 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5641 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5701 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5761 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5821 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5881 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5941 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6001 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6061 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6121 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6181 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6241 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6301 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6361 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6421 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6481 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6541 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6601 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6661 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6721 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6781 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6841 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6901 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6961 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7021 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7081 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7141 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7201 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7261 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7321 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7381 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7441 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7501 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7561 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7621 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7681 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7741 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7801 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7861 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7921 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7981 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8041 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     8101 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8161 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8221 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8281 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8341 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8461 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8521 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8581 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8641 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8701 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8761 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8821 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8881 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8941 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9001 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9721 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9781 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9841 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9901 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9961 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10021 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10081 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10141 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10201 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10261 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10321 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10381 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10441 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10501 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10561 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10621 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10681 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10741 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10801 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10861 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10921 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10981 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11041 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11101 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    11161 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11221 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11281 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
