LOCUS       V026499                10515 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026499
VERSION     V026499
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..5369
                     /label="CDCA2(NM_152562)"
                     /note="CDCA2(NM_152562)"
                     /gene="CDCA2"
     CDS             5376..5441
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5485..5696
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5709..5977
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6004..6603
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6610..7198
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7272..7505
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7577..7698
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7717..7852
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7890..7906)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7914..7930
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7938..7968)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7983..8004
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8292..8880)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9051..9911)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9912..10016)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10490..10506
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atggatgcca attcaaaaga caagccccct gaaaccaagg
     2341 agtctgcaat gaataatgct ggaaatgcct ctttcatttt gggaactggg aagattgtga
     2401 ctcctcagaa gcatgccgaa ttacctccta atccttgcac accagatact tttaaatcac
     2461 ctttgaactt ttccacagta accgtagagc aattgggaat tacacctgaa agctttgtta
     2521 ggaactctgc aggaaagtca tcatcctacc ttaaaaaatg tagacgacgt tctgcagtcg
     2581 gtgctcgggg ctctcctgaa acaaaccatc tgattcgttt cattgctcgg cagcaaaata
     2641 taaagaatgc taggaaatct cctttggcac aagattctcc ttcccagggc agccctgcac
     2701 tgtatcgaaa tgttaacact ttaagagaac gaatatcagc cttccagtca gcttttcact
     2761 ccataaagga aaacgagaaa atgaccggct gtctggaatt ctcagaggca ggaaaagagt
     2821 ccgagatgac agacttgacc agaaaggaag gtctcagcgc ttgccagcag tctgggttcc
     2881 ctgcagtgtt gtcctccaaa cgtcggagaa tatcctatca gagagactct gatgaaaatc
     2941 tgacggatgc tgaaggaaaa gtaattggtc tccagatatt caatattgat acagacagag
     3001 catgtgcagt tgaaacttct gtagatcttt ctgagatatc atctaaactt ggttcaacac
     3061 agtctggatt tttagttgaa gagtctcttc ccctttcaga gctcacagag acttcaaatg
     3121 cactaaaggt tgctgactgt gtagtgggca aaggatcaag tgatgccgtt tcgcctgaca
     3181 cgttcacagc agaagtgagc tcagacgcag tccctgatgt caggtcacca gctactccag
     3241 cctgcaggag ggaccttccc acccccaaga cctttgtact tcgttctgta ctgaagaaac
     3301 cctctgttaa gatgtgtcta gagagcttac aggaacactg taacaacctc tatgatgatg
     3361 atgggactca tccgagctta atctcaaatc tcccaaactg ttgcaaagag aaagaagcag
     3421 aagatgaaga aaattttgaa gcacctgcct ttctaaatat gaggaagagg aagagagtta
     3481 cttttggaga ggacttaagc ccggaagtgt ttgatgaatc tttgccagca aatactccat
     3541 tgcgtaaagg aggaacacct gtttgtaaaa aagacttcag tggtctcagt tccctgctgc
     3601 ttgagcagtc acctgttcct gagccattac ctcaaccaga ttttgatgac aagggggaga
     3661 atcttgaaaa catagaacca cttcaagtat catttgccgt tctcagttct cctaataaat
     3721 catcaatctc tgagaccctt tcaggcactg atacctttag ttcttcaaat aaccatgaga
     3781 aaatatcctc tcctaaagtt ggtagaataa caaggacttc taacagaaga aatcaattgg
     3841 tcagtgttgt agaagagagt gtttgcaact tattgaatac agaagttcag ccttgtaaag
     3901 aaaagaaaat taataggagg aagtctcaag aaacaaagtg tacaaagaga gcacttccta
     3961 agaagagtca ggttttaaaa agttgcagaa agaagaaagg aaagggaaag aaaagtgttc
     4021 agaaatcttt atatggggaa agagacattg cttctaagaa gcccctcctc agtcctattc
     4081 ccgagctgcc tgaagtccct gagatgacac cttccattcc gagcatccga agactgggtt
     4141 caggttattt cagttcaaat ggcaaactgg aagaagtgaa gactcctaaa aatccagtga
     4201 aaagaaagga tcttttgcgt catgacccag atttgcatat gcatcaaggc tatgataaat
     4261 atgatgtctc tgaattctgc tcttatataa aaagttcctc atcgcttggc aatgctactt
     4321 ctgatgaaga tccaaataca aatataatga acattaatga aaataaaaat attccaaaag
     4381 caaaaaataa gtcagaaagt gaaaatgaac caaaagctgg aactgacagt cctgtttctt
     4441 gtgcttctgt aactgaagaa cgtgtggcat cagatagtcc caaacctgct ctgaccctgc
     4501 agcagggtca agaattttct gctggtggtc aaaatgcaga aaacctttgt cagttcttta
     4561 aaatttcacc agatttaaac ataaagtgtg aaagaaagga tgacttctta ggagctgcag
     4621 aaggaaaact gcaatgcaat cgtttaatgc ctaattcaca aaaagactgt cattgtttag
     4681 gagatgtctt aattgaaaat acgaaagaat ctaaaagcca gagtgaggat ttgggaagaa
     4741 aacccatgga aagtagcagt gttgtgagtt gcagagacag gaaagataga agacgttcca
     4801 tgtgttattc tgatggtcga agtttacatt tggaaaaaaa tggaaatcac acaccatcct
     4861 ccagtgtggg cagctctgta gaaattagtt tagaaaattc tgaactgttt aaagatttgt
     4921 ctgatgccat tgagcaaacc tttcagagga gaaatagtga aaccaaagtg cgacgtagca
     4981 cgaggctaca gaaggattta gaaaacgaag gtcttgtatg gatttcactt ccacttcctt
     5041 ccacttccca aaaagccaaa agaagaacaa tatgtacatt tgacagcagt ggatttgaaa
     5101 gtatgtctcc cataaaagaa actgtgtcct ccagacaaaa accgcagatg gcacctcccg
     5161 tctcagatcc agaaaacagc cagggccctg ctgctggttc ttccgatgaa cctggtaaga
     5221 ggaggaagag cttttgtata tctacacttg caaatactaa agccacttcc cagttcaaag
     5281 gctaccggag aagatcctct cttaatggga agggagagag ctctctgact gccttggaaa
     5341 ggattgaaca taatggagaa agaaagcagg gatccgacta caaagaccat gacggtgatt
     5401 ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg cgaaggatct
     5461 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     5521 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
     5581 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     5641 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga
     5701 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     5761 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     5821 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     5881 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     5941 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc
     6001 tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
     6061 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
     6121 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6181 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6241 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6301 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6361 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6421 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6481 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6541 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6601 tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6661 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6721 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6781 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6841 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6901 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6961 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7021 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7081 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7141 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7201 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7261 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg
     7321 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7381 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7441 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7501 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7561 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7621 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7681 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt
     7741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7801 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     7861 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7921 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7981 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8041 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8101 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8161 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8221 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8281 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8341 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8401 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8461 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8521 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8581 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8641 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8701 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8761 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8821 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10021 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10081 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    10141 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10201 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    10261 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    10321 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
    10381 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10441 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    10501 gccagtgcca agctg
//
