LOCUS       V033165                11033 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033165
VERSION     V033165
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5034
                     /label="CDH2(NM_001792)"
                     /note="CDH2(NM_001792)"
                     /gene="CDH2"
     CDS             5041..5067
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5071..5097
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5101..5127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5192..5403
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5416..5684
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5713..5722
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5743..6405
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6475..6528
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6529..7128
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7129..7716
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7790..8023
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8095..8216
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8235..8370
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8408..8424)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8432..8448
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8456..8486)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8501..8522
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8810..9398)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9569..10429)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10430..10534)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11008..11024
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt gccggatagc gggagcgctg
     2341 cggaccctgc tgccgctgct ggcggccctg cttcaggcgt ctgtagaggc ttctggtgaa
     2401 atcgcattat gcaagactgg atttcctgaa gatgtttaca gtgcagtctt atcgaaggat
     2461 gtgcatgaag gacagcctct tctcaatgtg aagtttagca actgcaatgg aaaaagaaaa
     2521 gtacaatatg agagcagtga gcctgcagat tttaaggtgg atgaagatgg catggtgtat
     2581 gccgtgagaa gctttccact ctcttctgag catgccaagt tcctgatata tgcccaagac
     2641 aaagagaccc aggaaaagtg gcaagtggca gtaaaattga gcctgaagcc aaccttaact
     2701 gaggagtcag tgaaggagtc agcagaagtt gaagaaatag tgttcccaag acaattcagt
     2761 aagcacagtg gccacctaca aaggcagaag agagactggg tcatccctcc aatcaacttg
     2821 ccagaaaact ccaggggacc ttttcctcaa gagcttgtca ggatcaggtc tgatagagat
     2881 aaaaaccttt cactgcggta cagtgtaact gggccaggag ctgaccagcc tccaactggt
     2941 atcttcatta tcaaccccat ctcgggtcag ctgtcggtga caaagcccct ggatcgcgag
     3001 cagatagccc ggtttcattt gagggcacat gcagtagata ttaatggaaa tcaagtggag
     3061 aaccccattg acattgtcat caatgttatt gacatgaatg acaacagacc tgagttctta
     3121 caccaggttt ggaatgggac agttcctgag ggatcaaagc ctggaacata tgtgatgacc
     3181 gtaacagcaa ttgatgctga cgatcccaat gccctcaatg ggatgttgag gtacagaatc
     3241 gtgtctcagg ctccaagcac cccttcaccc aacatgttta caatcaacaa tgagactggt
     3301 gacatcatca cagtggcagc tggacttgat cgagaaaaag tgcaacagta tacgttaata
     3361 attcaagcta cagacatgga aggcaatccc acatatggcc tttcaaacac agccacggcc
     3421 gtcatcacag tgacagatgt caatgacaat cctccagagt ttactgccat gacgttttat
     3481 ggtgaagttc ctgagaacag ggtagacatc atagtagcta atctaactgt gaccgataag
     3541 gatcaacccc atacaccagc ctggaacgca gtgtacagaa tcagtggcgg agatcctact
     3601 ggacggttcg ccatccagac cgacccaaac agcaacgacg ggttagtcac cgtggtcaaa
     3661 ccaatcgact ttgaaacaaa taggatgttt gtccttactg ttgctgcaga aaatcaagtg
     3721 ccattagcca agggaattca gcacccccct cagtcaactg caaccgtgtc tgttacagtt
     3781 attgacgtaa atgaaaaccc ttattttgcc cccaatccta agatcattcg ccaagaagaa
     3841 gggcttcatg ccggtaccat gttgacaaca ttcactgctc aggacccaga tcgatatatg
     3901 cagcaaaata ttagatacac taaattatct gatcctgcca attggctaaa aatagatcct
     3961 gtgaatggac aaataactac aattgctgtt ttggaccgag aatcaccaaa tgtgaaaaac
     4021 aatatatata atgctacttt ccttgcttct gacaatggaa ttcctcctat gagtggaaca
     4081 ggaacgctgc agatctattt acttgatatt aatgacaatg cccctcaagt gttacctcaa
     4141 gaggcagaga cttgcgaaac tccagacccc aattcaatta atattacagc acttgattat
     4201 gacattgatc caaatgctgg accatttgct tttgatcttc ctttatctcc agtgactatt
     4261 aagagaaatt ggaccatcac tcggcttaat ggtgattttg ctcagcttaa tttaaagata
     4321 aaatttcttg aagctggtat ctatgaagtt cccatcataa tcacagattc gggtaatcct
     4381 cccaaatcaa atatttccat cctgcgtgtg aaggtttgcc agtgtgactc caacggggac
     4441 tgcacagatg tggacaggat tgtgggtgcg gggcttggca ccggtgccat cattgccatc
     4501 ctgctctgca tcatcatcct gcttatcctt gtgctgatgt ttgtggtatg gatgaaacgc
     4561 cgggataaag aacgccaggc caaacaactt ttaattgatc cagaagatga tgtaagagat
     4621 aatattttaa aatatgatga agaaggtgga ggagaagaag accaggacta tgacttgagc
     4681 cagctgcagc agcctgacac tgtggagcct gatgccatca agcctgtggg aatccgacga
     4741 atggatgaaa gacccatcca cgccgagccc cagtatccgg tccgatctgc agccccacac
     4801 cctggagaca ttggggactt cattaatgag ggccttaaag cggctgacaa tgaccccaca
     4861 gctccaccat atgactccct gttagtgttt gactatgaag gcagtggctc cactgctggg
     4921 tccttgagct cccttaattc ctcaagtagt ggtggtgagc aggactatga ttacctgaac
     4981 gactgggggc cacggttcaa gaaacttgct gacatgtatg gtggaggtga tgacctcgag
     5041 tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga ttatgctagc
     5101 tatccttatg atgtcccaga ctacgcatag ggatccctgc gatatccagc gcggccgcga
     5161 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5221 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     5281 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5341 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5401 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5461 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5521 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5581 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5641 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5701 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5761 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5821 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5881 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5941 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     6001 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     6061 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     6121 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     6181 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     6241 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     6301 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     6361 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     6421 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     6481 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6721 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     7021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     7081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     7141 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7201 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7261 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7321 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7381 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7441 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7501 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7561 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7621 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct
     7681 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact
     7741 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7801 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     8041 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     8101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8221 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8281 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8341 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     8401 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9241 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9481 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9541 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9601 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9661 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9721 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9781 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9841 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9901 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9961 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10021 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10081 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10141 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10201 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10261 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10321 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10381 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10441 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10501 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10561 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10621 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10681 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10741 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10801 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10861 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10921 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10981 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
